Hemocyte-Mediated Shell Mineralization in the Eastern Oyster

Science

Volumn 304, Issue 5668, 2004, Pages 297-300

  Mount, Andrew S ^a   Wheeler, A P ^a   Paradkar, Rajesh P ^b,d   Snider, D ^c  

^a CLEMSON UNIVERSITY   (United States)

^b AMRL   (United States)

^c NONE   (United States)

^d DOW CHEMICAL COMPANY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CALCIUM COMPOUNDS; CARBONATES; CELLS; SCANNING ELECTRON MICROSCOPY; X RAY ANALYSIS;

SHELL CRYSTAL PRODUCTION; X RAY MICROANALYSIS;

BIODIVERSITY;

CALCIUM;

MOLLUSC; SHELL;

ARTICLE; BLOOD CELL; CELL REGENERATION; CRYSTALLIZATION; EXTRACELLULAR MATRIX; FLUORESCENCE; GRANULOCYTE; GROWTH REGULATION; MINERALIZATION; MOLLUSCAN SHELL; NONHUMAN; OYSTER; PRIORITY JOURNAL; PROCESS MODEL; SCANNING ELECTRON MICROSCOPY; VITAL STAIN; X RAY MICROANALYSIS;

ANIMALS; CALCIFICATION, PHYSIOLOGIC; CALCIUM CARBONATE; CRYSTALLIZATION; ELECTRON PROBE MICROANALYSIS; FLUORESCEINS; GRANULOCYTES; HEMOCYTES; MICROSCOPY, ELECTRON, SCANNING; MICROSCOPY, FLUORESCENCE; OSTREIDAE;

CRASSOSTREA VIRGINICA; MOLLUSCA; OSTREIDAE;

EID: 1842482361     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1090506     Document Type: Article

References (34)

1. K. Simklss, K. M. Wilbur, Biomineralization, Cell Biology and Mineral Deposition (Academic Press, New York, 1989).
2. A. P. Wheeler, in Calcification in Biological Systems, E. Bonucci, Ed. (CRC Press, Boca Raton, FL, 1992), pp. 179-216.
3. H. A. Lowenstam, S. Weiner, On Biomineralization (Oxford Univ. Press, Oxford, 1989), pp. 74-175.
4. J. Alzenberg, D. A. Muller, J. L. Grazul, D. R. Hamann, Science 299, 1205 (2003).
5. A. M. Belcher et al., Nature 381, 56 (1996).
6. S. Mann, B. R. Heywood, S. Rajam, J. D. Birchall. Nature 334, 692 (1988).
7. G. S. Falini, S. Albeck, S. Wiener, L. Addadi, Science 271, 67 (1996).
8. L. A. Addadi, J. Moradian, E. Shay, N. G. Maroudes, S. Weiner, Proc. Natl. Acad. Sci. U.S.A. 84, 2732 (1987).
9. E. M. Greenfield, D. C. Wilson, M. A. Crenshaw, Am. Zool. 24, 925 (1984).
10. M. A. Crenshaw, Biol. Bull. 143, 506 (1972).
11. A. S. Mount, thesis, Clemson University (1999).
12. C. S. Sikes, A. P. Wheeler, A. Wierzbicki, A. S. Mount, R. A. Dillaman, Biol. Bull. 198, 50 (2000).
13. L. H. Jodrey, Biol. Bull. 104, 398 (1953).
14. K. M. Wilbur, L. H. Jodrey, Biol. Bull. 103, 269 (1952).
15. K. M. Wilbur, in Physiology of Mollusca, K. M. Wilbur, C. M. Yonge, Eds. (Academic Press, New York, 1964), vol. 1, pp. 242-282.
16. A. F. Eble, in The Eastern Oyster, Crassostrea virginica, V. S. Kennedy, R. I. E. Newell, A. F. Eble, Eds. (Maryland Sea Grant College, College Park, MD, 1996), pp. 271-298.
17. T. C. Cheng, in Invertebrate Blood Cells, N. A. Ratcliffe, A. F. Rowley, Eds. (Academic Press, London, 1981), pp. 233-300.
18. E. Gosling, Bivalve Molluscs, Biology, Ecology and Culture (Blackwell Publishing, Oxford, 2003).
19. T. C. Cheng, in The Eastern Oyster, Crassostrea virginica, V. S. Kennedy, R. I. E. Newell, A. F. Eble, Eds. (Maryland Sea Grant College, College Park, MD, 1996), pp. 299-333.
20. M. Justus, S. Patel, A. S. Mount, poster presented at the 6th International Conference on Shellfish Restoration, Charleston, SC, 20 to 24 November 2002.
21. M. G. McCormick-Ray, T. Howard, J. Invertebr. Pathol. 58, 219 (1991).
22. P. M. Hine, Fish Shellfish Immunol. 9, 367 (1999).
23. Materials and methods are available as supporting material on Science Online.
24. M. R. Carriker, R. E. Palmer, R. S. Prezant, Proc. Natl. Shellfish Assoc. 70, 139 (1980).
25. M. R. Carriker, in The Eastern Oyster, Crassostrea virginica, V. S. Kennedy, R. I. E. Newell, A. F. Eble, Eds. (Maryland Sea Grant College, College Park, MD, 1996), pp. 75-167.
26. P. S. Galtsoff, Fish Bull. 64, 1 (1964).
27. K. M. Wilbur, A. S. M. Saleuddin, in The Mollusca, K. M. Wilbur, Ed. (Academic Press, New York, 1983), vol. 4, part 1, pp. 236-288.
28. S. E. Ford, S. A. Kanaley, D. T. Littlewood, J. Invertebr. Pathol. 61, 49 (1993).
29. A. P. Wheeler, P. L. Blackwelder, K. M. Wilbur, Biol. Bull. 148, 472 (1975).
30. N. Watabe, In The Mollusca, K. M. Wilbur, Ed. (Academic Press, New York, 1983), vol. 4, part 1, pp. 289-317.
31. R. J. Kingsley, Am. Zool. 24, 833 (1984).
32. A. H. Scheltema, Biol. Bull. 184, 57 (1993).
33. A. H. Scheltema, C. Schander, Biol. Bull. 198, 121 (2000).
34. We thank E. Ruppert, R. Montanucci, and D. Bushek for careful reading and suggestions that have improved the quality of this manuscript. We also thank J. Hatchell for his efforts in cell enumeration and construction of the oyster holding facility; B. Kay for technical help with EM; J. Mount for assistance in revised figure preparation: and T. McNutt Scott, M. Johnstone, S. Patel, and M. Justus for invaluable discussions. We gratefully acknowledge support from NSF under award no. 20020445.