Purification and characterization of a recombinant listeriolysin O expressed in Escherichia coli and possible diagnostic applications

Journal of Biotechnology

Volumn 109, Issue 1-2, 2004, Pages 13-20

  Giammarini, Camilla ^a   Andreoni, Francesca ^a   Amagliani, Giulia ^a   Casiere, Annarita ^b   Barocci, Simone ^b   Magnani, Mauro ^a  

^a UNIVERSITY OF URBINO   (Italy)

^b ISTITUTO ZOOPROFILATTICO SPERIMENTALE DELL UMBRIA E DELLE MARCHE   (Italy)

Author keywords

ELISA; Listeriolysin O; Listeriosis; Recombinant protein

Indexed keywords

CELL CULTURE; ESCHERICHIA COLI; PROTEINS; PURIFICATION; SYNTHESIS (CHEMICAL); TOXIC MATERIALS;

TOXINS; VIRULENCE FACTORS;

BIOTECHNOLOGY;

BACTERIAL TOXIN; LISTERIOLYSIN; RECOMBINANT LISTERIOLYSIN O; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; ANIMAL MODEL; BACTERIUM CULTURE; CONFERENCE PAPER; CONTROLLED STUDY; CULTURE MEDIUM; DIAGNOSTIC TEST; DIAGNOSTIC VALUE; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; EXPERIMENTAL INFECTION; LISTERIA MONOCYTOGENES; LISTERIOSIS; METHODOLOGY; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN ISOLATION; PROTEIN PURIFICATION; PROTEIN SYNTHESIS; SCALE UP; SHEEP; TOXIN ANALYSIS;

ANIMALS; BACTERIAL TOXINS; CHOLESTEROL; ENZYME-LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; HEAT-SHOCK PROTEINS; HEMOLYSIN PROTEINS; HEMOLYSIS; HYDROGEN-ION CONCENTRATION; LISTERIA INFECTIONS; LISTERIA MONOCYTOGENES; MERCURIC CHLORIDE; P-CHLOROMERCURIBENZOIC ACID; RECOMBINANT PROTEINS; SHEEP; SHEEP DISEASES;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; LISTERIA; LISTERIA MONOCYTOGENES; POSIBACTERIA;

EID: 1842478330     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2003.10.023     Document Type: Conference Paper

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