The Analysis of Synthetic Organic, Neutral Polymers Using Nonaqueous Capillary Gel Electrophoresis (NACGE)

Journal of Liquid Chromatography and Related Technologies

Volumn 27, Issue 6, 2004, Pages 939-964

  Li, Guodong ^a   Zhou, Xiaojuan ^a   Wang, Yonghui ^a   Krull, Ira S ^a   Mistry, Kavita ^b   Grinberg, Nelu ^b   Cortes, Hernan ^c  

^a NORTHEASTERN UNIVERSITY   (United States)

^b MERCK RESEARCH LABORATORIES   (United States)

^c DOW CHEMICAL COMPANY   (United States)

Author keywords

Capillary electrophoresis; Molecular weight determinations; Nonaqueous capillary gel electrophoresis; Synthetic organic polymers

Indexed keywords

ELECTRIC POTENTIAL; ELECTROPHORESIS; GELS; LIQUID CHROMATOGRAPHY; MOLECULAR WEIGHT; ORGANIC SOLVENTS;

BUFFER ADDITIVES; ORGANIC GELS;

ORGANIC POLYMERS;

MACROGOL; ORGANIC COMPOUND; POLY(METHYL METHACRYLATE); POLYMER; POLYSTYRENE;

ANALYTIC METHOD; ARTICLE; CAPILLARY ELECTROCHROMATOGRAPHY; CHEMICAL ANALYSIS; ELECTROOSMOSIS; GEL ELECTROPHORESIS; GEL PERMEATION CHROMATOGRAPHY; HYDROPHOBICITY; MOLECULAR WEIGHT; NONAQUEOUS CAPILLARY GEL ELECTROPHORESIS; PHOTOMETRY; PROTEIN DNA INTERACTION; SIGNAL NOISE RATIO; VISCOSITY;

EID: 1842427369     PISSN: 10826076     EISSN: None     Source Type: Journal    
DOI: 10.1081/JLC-120030171     Document Type: Article

References (46)

1. Landers, J.P., Ed. CRC Handbook of Capillary Electrophoresis: Principles, Methods, and Applications; CRC Press: Boca Raton, FL, 1997.
2. Baker, D.R. Capillary electrophoresis. In Techniques in Analytical Chemistry Series; J. Wiley & Sons, Inc.: New York, 1995.
3. Camilleri, P. (Ed.) Capillary Electrophoresis, Theory and Practice; CRC Press: Boca Raton, 1993.
4. Mosher, R.A.; Thormann, W. The Dynamics of Electrophoresis; VCH Publishers: Weinheim, FRG, 1992; Chapter 7.
5. Altria, K.D., Ed. Capillary Electrophoresis Guidebook: Principles, Operation, and Applications; Human Press: Totowa, NJ, 1996.
6. Weinberger, R.; Lombardi, R. Method Development, Optimization and Troubleshooting for High Performance Capillary Electrophoresis; Simon & Schuster Custom Publishing: Needham Heights, MA, 1997.
7. Weinberger, R. Practical Capillary Electrophoresis, 2nd Ed.; Academic Press: San Diego, CA, 2000.
8. Snyder, L.R.; Kirkland, J.J.; Glajch, J.L. Practical HPLC Method Development, 2nd Ed.; John Wiley & Sons: New York, 1997; Chapter 11.
9. (a) Barth, H.G., Mays, J.W., Eds. Modern Methods of Polymer Characterization; John Wiley & Sons: New York, 1991;
10. (b) Mori, S.; Barth, H.G. Size Exclusion Chromatography; Springer-Verlag: Berlin, 1999.
11. Dubin, P.D., Ed. Aqueous Size-Exclusion Chromatography; Elsevier Science Publishers: Amsterdam, 1988.
12. Brown, P.R., Hartwick, R.A., Eds. High Performance Liquid Chromatography; John Wiley & Sons: New York, 1989; Chapter 6.
13. Cazes, J., Ed. Encyclopedia of Chromatography; Marcel Dekker: New York, 2001.
14. Cazes, J., Ed. Liquid Chromatography of Polymers and Related Materials; Marcel Dekker: New York, 1977.
15. Cazes, J., Delamare, X., Eds. Liquid Chromatography of Polymers and Related Materials, II; Marcel Dekker: New York, 1980.
16. Wu, C.-S., Ed. Column Handbook for Size Exclusion Chromatography; Academic Press: San Diego, CA, 1999.
17. Sahota, R.S.; Khaledi, M. Nonaqueous capillary electrophoresis. Anal. Chem. 1994, 66, 1141.
18. Wright, P.B.; Lister, A.S.; Dorsey, J.G. Behavior and use of nonaqueous media without supporting electrolyte in capillary electrophoresis and capillary electrochromatography. Anal. Chem. 1997, 69, 3251.
19. Steiner, F.; Hassel, M. Nonaqueous capillary electrophoresis: a versatile completion of electrophoretic separation techniques. Electrophoresis 2000, 21, 3994.
20. Whitaker, K.W.; Sepaniak, M.J. Nonaqueous packed capillary electrokinetic chromatographic separations of large polycyclic aromatic hydrocarbons and fullerenes. Electrophoresis 1994, 15, 1341.
21. Roed, L.; Lundanes, E.; Greibrokk, T. Nonaqueous electrochromatography on continuous, bed columns of sol-gel bound large-pore C30 material. J. Microcol. Sepns. 2000, 12, 561.
22. Maruska, A.; Pyell, U. Capillary electrochromatography: normal-phase mode using silica gel and cellulose-based packing materials. J. Chromatogr., A 1997, 782, 167.
23. Venema, E.; Kraak, J.C.; Poppe, H.; Tijssen, R. Electrically driven capillary size exclusion chromatography. Chromatographia 1998, 48, 347.
24. Lindberg, P.; Roeraade, J. Gel matrixes in N-methylformamide for separation of DNA fragments. J. Liq. Chrom. & Rel. Technol. 1999, 22 (2), 307.
25. (a) Guttman, A.; Horvath, J.; Cooke, N. Influence of temperature on the sieving effect of different polymer matrixes in capillary SDS gel electrophoresis of proteins. Anal. Chem. 1993, 65, 199;
26. (b) Guttman, A. On the seperation mechanism of capillary sodium dodecyl sulfate-gel electrophoresis of proteins. Electrophoresis 1995, 16, 611;
27. (c) Benedek, K.; Guttman, A. Ultra-fast high-performance capillary sodium dodecyl suflate gel electrophoresis of proteins. J. Chromatogr., A 1994, 680, 375.
28. Ganzler, K.; Greve, K.S.; Cohen, A.S.; Karger, B.L.; Guttman, A.; Cooke, N.C. High-performance capillary electrophoresis of SDS-protein complexes using UV-transparent polymer networks. Anal. Chem. 1992, 64, 2665.
29. Grosche, O.; Bohrisch, J.; Wendler, U.; Jaeger, W.; Engelhardt, H. Characterization of synthetic polyelectrolytes by capillary electrophoresis. J. Chromatogr., A 2000, 894, 105.
30. Engelhardt, H.; Grosche, O. Advances in Polymer Science; Schmidt, M., Ed.; Springer-Verlag: Weinheim, Germany, 2000; Vol. 150; Chapter 3.
31. Li, W.; Zhong, Y.; Lin, B.; Su, Z. Characterization of polyethylene glycol-modified proteins by semi-aqueous capillary electrophoresis. J. Chromatogr., A 2001, 905, 299.
32. Esaka, Y.; Inagaki, S.; Uchida, D.; Goto, M.; Kano, K. Polyacrylamides as hydrophilic selectors in non-aqueous capillary electrophoresis. J. Chromatogr., A 2001, 905, 291.
33. Poli, J.B.; Schure, M.R. Separation of poly(styrenesulfonates) by capillary electrophoresis with polymeric additives. Anal. Chem. 1992, 64, 896.
34. Schure, M.R.; Murphy, R.E.; Klotz, W.L.; Lau, W. High-performance capillary gel electrochromatography with replaceable media. Anal. Chem. 1998, 70, 4985.
35. Clos, H.N.; Engelhardt, H. Separations of anionic and cationic synthetic polyelectrolytes by capillary gel electrophoresis. J. Chromatogr., A 1998, 802, 149.
36. Hoagland, D.A.; Muthukumar, M. Evidence for entropic barrier transport of linear, star, and ring macromolecules in electrophoresis gels. Macromolecules 1992, 25, 6696.
37. Smisek, D.L.; Hoaglund, D.A. Electrophoresis of flexible macromolecules: evidence for a new mode of transport in gels. Science 1990, 248, 1221.
38. Gao, J.Y.; Dubin, P.L.; Sato, T.; Morishima, Y. Separation of polyelectrolytes of variable compositions by free-zone capillary electrophoresis. J. Chromatogr. 1997, 766, 233.
39. Hoagland, D.A.; Smisek, D.L.; Chen, D.Y. Gel and free solution electrophoresis of variably charged polymers. Electrophoresis 1996, 17, 1151.
40. Cottet, H.; Gareil, P. On the use of the activation energy concept to investigate analyte and network deformations in entangled polymer solution capillary electrophoresis of synthetic polyelectrolytes. Electrophoresis 2001, 22, 684.
41. Dolnik, V.; Gurske, W.A.; Padua, A. Galactomannans as a sieving matrix in capillary electrophoresis. Electrophoresis 2001, 22, 707.
42. Tseng, W.L.; Lin, W.; Chang, H.T. Improved separation of microheterogeneities and isoforms of proteins by capillary electrophoresis using segmental filling with SDS and PEO in the background electrolyte. Anal. Chem. 2002, 74, 4828.
43. Mistry, K.; Krull, I.S.; Grinberg, N. Size-exclusion capillary electrochromatography separation of polysaccharides using polymeric stationary phases. Electrophoresis 2003, 24, 1753.
44. (a) Krull, I.S.; Stevenson, R.; Mistry, K.; Swartz, M.E. Capillary Electrochromatography (CEC) and Pressurized Flow CEC (PEC) or Electro-HPLC, An Introduction; HNB Publishers: New York, May 2000;
45. (b) Mistry, K.; Grinberg, N.; Krull, I.S. Capillary electrochromatography: an alternative to HPLC and CE. J. Sepn. Sci. 2002, 25 (15-17), 935.
46. Mistry, K.; Cortes, H.; Meunier, D.; Schmidt, C.; Grinberg, N.; Krull, I.S. Nonaqeous capillary electrochromatographic separation of synthetic neutral polymers by size exclusion chromatography using polymeric stationary phases. Anal. Chem. 2002, 74, 617.