Probing ion channel conformational dynamics using simultaneous single-molecule ultrafast spectroscopy and patch-clamp electric recording

Applied Physics Letters

Volumn 84, Issue 10, 2004, Pages 1792-1794

  Harms, Greg ^a,b   Orr, Galya ^a   Lu, H Peter ^a  

^a PACIFIC NORTHWEST NATIONAL LABORATORY   (United States)

^b UNIVERSITY OF WÜRZBURG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ANISOTROPY; AVALANCHE DIODES; CHARGE COUPLED DEVICES; CONFORMATIONS; ELECTRIC CURRENTS; ENERGY TRANSFER; FLUORESCENCE; LIPIDS; OPTICAL FILTERS; PHOTONS; QUENCHING;

ELECTRIC RECORDINGS; PATCH-CLAMP CONFOCAL FLUORESCENCE MICROSCOPY (PCCFM);

MOLECULAR DYNAMICS;

EID: 1842424308     PISSN: 00036951     EISSN: None     Source Type: Journal    
DOI: 10.1063/1.1652228     Document Type: Article

References (13)

1. B. Hille, Ion Channels of Excitable Membranes, 3rd ed. (Sinauer, MA, 2001).
2. B. Sakmann and E. Neher, Single Channel Recordings, 2nd ed. (Kluwer, New York, 1995).
3. G. S. Harms, G. Orr, M. Montal, B. D. Thrall, S. Colson, and H. P. Lu, Biophys. J. 85, 1826 (2003).
4. V. T. Borisenko, J. Lougheed, J. Hesse, E. Fureder-Kitzmuller, N. Fertig, J. C. Behrends, G. A. Woolley, and G. J. Schutz, Biophys. J. 84, 612 (2003).
5. T. Ide, Y. Takeuchi, and T. Yanagida, Single Mol. 3, 33 (2002).
6. A. Sonnleitner, L. M. Mannuzzu, S. Terakawa, and E. Y. Isacoff, Proc. Natl. Acad. Sci. U.S.A. 99, 12759 (2002).
7. G. Orr, G. S. Harms, B. D. Thrall, M. Montal, S. Colson, and H. P. Lu, Biophys. J. 82, 255a (2002).
8. J. R. Fries, L. Brand, C. Eggeling, M. Kollner, and C. A. M. Seidel, J. Phys. Chem. A 102, 6601 (1998).
9. Y. Chen, D. Hu, E. Vorpagel, and H. P. Lu, J. Phys. Chem. B 107, 7947 (2003).
10. D. Hu and H. P. Lu, J. Phys. Chem. B 107, 618 (2003).
11. Measurements were made with the correction of a "g factor" for the differences in detection efficiencies between the parallel and perpendicular detections. The data could subsequently be binned again and analyzed into two periods corresponding to open and closed states of the channel.
12. A. J. Cross and G. R. Fleming, Biophys. J. 46, 45 (2003).
13. G. V. Milochevsky and P. C. Gordon, Biophys. J. 86, 92 (2004).