Subcellular localization of G-proteins in primary-cultured mouse preadipocytes and adipocytes

Journal of Cellular Biochemistry

Volumn 65, Issue 2, 1997, Pages 259-266

  Bégin Heick, Nicole ^a   Black, Marsha A ^a,b   Gaffield, Johanna ^a   Cadrin, Monique ^a,c  

^a UNIVERSITY OF OTTAWA   (Canada)

^b Office de la Properiete intellectuelle du Canada   (Canada)

^c UNIVERSITÉ DU QUÉBEC À TROIS RIVIÈRES   (Canada)

Author keywords

actin; adenylyl cyclase; antibodies; differentiation; fat accumulation; G(i) ; G(s) ; G ; immunofluorescence microscopy; lipid; obese; obesity; stress fibres; subcellular localization; vimentin

Indexed keywords

5 [2 [[2 (3 CHLOROPHENYL) 2 HYDROXYETHYL]AMINO]PROPYL] 1,3 BENZODIOXOLE 2,2 DICARBOXYLIC ACID; ADENYLATE CYCLASE; GUANINE NUCLEOTIDE BINDING PROTEIN; GUANOSINE TRIPHOSPHATE; ISOPRENALINE;

ADIPOCYTE; ANIMAL CELL; ARTICLE; CELL DIFFERENTIATION; CELL INTERACTION; CELLULAR DISTRIBUTION; CONTROLLED STUDY; MALE; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROADIPOCYTE; PROTEIN LOCALIZATION; PROTEIN PROCESSING;

ANIMALIA;

EID: 1842404125     PISSN: 07302312     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-4644(199705)65:2<259::AID-JCB11>3.0.CO;2-D     Document Type: Article

References (38)

1. Ailhaud G, Grimaldi P, Negrel R (1992): Cellular and molecular aspects of adipose tissue development Annu Rev Nutr 12:207-233.
2. 1α. Science 262:1569-1572.
3. s and Gi) of adenylate cyclase in adipocyte membranes from lean and obese (ob/ob) mice. Biochem J 268:83-89.
4. i in adipocyte plasma membranes of genetically diabetic mice. Am J Physiol 263 (Cell Physiol 32):C121-C129.
5. 3-adrenergic receptors in mouse adipose tissue. Modulation by GTP and effects of obesity. J. Cell. Biochem. 58:464-473.
6. Birnbaumer M, Birnbaumer L (1994): Signal transduction by G proteins: 1994 edition. J Recept Signal Transduct Res 15:213-252.
7. Black MA, Bégm-Heick N (1995): Growth and maturation of primary-cultured adipocytes from lean and ob/ob mice. J Cell Biochem 58:455-463.
8. Blanchette-Mackie EJ, Dwyer NK, Barber T, Coxey RA, Takeda T, Rondinone CM, Theodorakis JL, Greenberg AS, Londos C (1995): Perilipin is located on the surface layer of intracellular lipid droplets in adipocytes. J Lipid Res 36:1211-1226.
9. o in primary cultures of neuronal and glial cells. J Neurosci 8:701-708.
10. Cadrin M, McFarlane-Anderson N, Bégin-Heick N (1994): Identification and localization of Giα3 in the clonal adipocyte cell lines HGFu and Ob17. Biochem Cell Biol 71:518-521.
11. Cadrin M, McFarlane-Anderson N, Harper ME, Gaffield J, Bégin-Heick N (1996): Comparison of the subcellular distribution of G-proteins in hepatocytes in situ and in primary cultures. J Cell Biochem 62:334-341.
12. Donaldson JG, Khan RA, Lippincott-Schwartz J, Klausner RD (1991): Bmdmg of ARF and beta-COP to Golgi membranes: Possible regulation by a trimeric G protein. Science 254:1197-1199.
13. Egan JJ, Greenberg AS, Chang M-K, Wek SA, Moos MC Jr, Londos C (1992): Mechanism of hormone-stimulated lipolysis in adipocytes: Translocation of hormone-sensitive lipase to the lipid storage droplet. Proc Natl Acad Sci USA 89:8537-8541.
14. 1 cells. Proc Natl Acad Sci U S A 87:4635-4639.
15. o in neurons. Cell Signal 1:107-123.
16. Gettys TW, Ramkumar V, Uhing R, Seger L, Taylor IL (1991): Alterations in mRNA levels, expression, and function of GTP-binding regulatory proteins in adipocytes from obese mice (C57B1/6J-ob/ob). J Biol Chem 266: 15949-15955.
17. Gettys TW, Ramkumar V, Surwit RS, Taylor IL (1995): Tissue-specific alterations in G protein expression in genetic versus diet-induced models of non-insulin-dependent diabetes mellitus in the mouse. Metabolism 44:771-778.
18. Greenberg AS, Egan JJ, Wek SA, Garty NB, Blanchette-Makie EJ, Londos C (1991): Perilipin, a major hormonaly regulated adipocyte-specific phosphoprotein associated with the periphery of lipid storage droplets. J Biol Chem 266:11341-11346.
19. Hepler JR, Gilman AG (1992): G proteins. Trends Biochem Sci 17:383-387.
20. Hidalgo J, Muñiz M, Velasco A (1995): Trimeric G proteins regulate the cytosol-induced redistribution of Golgi enzymes into the endoplasmic reticulum. J Cell Sci 108: 1805-1815.
21. Kilgeour E, Anderson NG (1993): Changes in the expression of guanine nucleotide-binding proteins during differentiation of 3T3-F442A cells in a hormonally defined medium. FEBS Lett 328:271-274.
22. i is localized to the insulin secretory granules of beta-cells and is involved in insulin exocytosis. J Biol Chem 270:12869-12876.
23. Kowluru A, Rabaglia ME, Muse KE, Metz SA (1994): Subcellular localization and kinetic characterization of guanine nucleotide binding proteins in normal rat and human pancreatic islets and transformed beta cells. Biochim Biophys Acta 1222:348-359.
24. Kowluru A, Seavy SE, Rhodes CJ, Metz SA (1996): A novel regulatory mechanism for trimeric GTP-binding proteins in the membrane and secretory granule fraction of human and rodent beta cells. Biochem J 313:97-107.
25. i: Visualization by immunofluorescent and immunogold labelling. Cell Regul 2:1097-1113.
26. Leyte A, Barr FA, Kehlenbach RH, Huttner WB (1992): Multiple trimeric G-proteins on the trans-Golgi network exert stimulatory and inhibitory effects on secretory vesicle formation. EMBO J 11:4795-4804.
27. Lipsky NG, Pagano RE (1985): A vital stain for the Golgi apparatus. Science 228:745-747.
28. McFarlane-Anderson N, Cadrin M, Bégin-Heick N (1993): Identification and localization of G-proteins in the clonal adipocyte cell lines HGFu and Ob17. J Cell Biochem 52:463-475.
29. Mélançon P, Glock BS, Malhdra V, Weidman PJ, Serafini T, Gleason ML, Orci L, Rothman JE (1987): Involvement of GTP-binding "G" proteins in transport through the Golgi stack. Cell 51:1053-1062.
30. Muntz KH, Sternweis PC, Gilman AG, Mumby SM (1992): Influence of γ subunit prenylation on association of guanine nucleotide-binding regulatory proteins with membranes. Mol Biol Cell 3:49-61.
31. Saffitz JE, Nash JA, Green KG, Luke RA, Ransnäs LA, Insel PA (1994): Immunoelectron microscopic identification of cytoplasmic and nuclear Gs alpha in S49 lymphoma cells. FASEB J 8:252-258.
32. Shillabeer G, Forden JM, Russell JC, Lau DCW (1990): Paradoxically slow preadipocyte replication and differentiation in corpulent rats. Am J Physiol 258 (Endocrinol Metab 21):E368-E376.
33. Stow JL, de Almeida JB (1993): Distribution and role of heterotrimeric G proteins in the secretory pathway of polarized epithelial cells. J Cell Sci Suppl 17:33-39.
34. Themmen APN, Hinrichs V, Birnbaumer M (1993): In situ assay of hormone-stimulated adenylyl cyclase in 96-well microtitration plates - an aide to rapid identification of transformed cell clones. J Recept Res 13:69-78.
35. Wang H, Berrios M, Malbon CC (1989): Indirect immunofluorescence localization of β-adrenergic receptors and G-proteins in human A431 cells. Biochem J 263:519-532.
36. Wedegaertner PB, Wilson PT, Bourne HR (1995): Lipid modification of trimeric G proteins. J Biol Chem 270:503-506.
37. Wilson BS, Komuro M, Farquhar MG (1994): Cellular variations in heterotrimeric G protein localization and expression in rat pituitary. Endocrinology 134:233-244.
38. Zhang Y, Proenca R, Maffei M, Barone M, Leopold L, Friedman JM (1994): Positional cloning of the mouse obese gene and its human homologue. Nature 372:425-432.