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Volumn 276, Issue 5311, 1997, Pages 407-409

Endosomal targeting by the cytoplasmic tail of membrane immunoglobulin

Author keywords

[No Author keywords available]

Indexed keywords

B LYMPHOCYTE ANTIGEN; IMMUNOGLOBULIN A; IMMUNOGLOBULIN G; IMMUNOGLOBULIN M; LYMPHOCYTE ANTIGEN RECEPTOR;

EID: 1842377430     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.276.5311.407     Document Type: Article
Times cited : (33)

References (27)
  • 1
    • 0028118624 scopus 로고
    • M. R. Gold and A. L. DeFranco, Adv. Immunol. 55, 221 (1994); C. M. Pleiman, D. D'Ambrosio, J. C. Cambier, Immunol. Today 15, 393 (1994); M. S. Neuberger et al., Immunol. Rev. 132, 147 (1993); M. Reth, Annu. Rev. Immunol. 10, 97 (1992).
    • (1994) Adv. Immunol. , vol.55 , pp. 221
    • Gold, M.R.1    DeFranco, A.L.2
  • 2
    • 0027931209 scopus 로고
    • M. R. Gold and A. L. DeFranco, Adv. Immunol. 55, 221 (1994); C. M. Pleiman, D. D'Ambrosio, J. C. Cambier, Immunol. Today 15, 393 (1994); M. S. Neuberger et al., Immunol. Rev. 132, 147 (1993); M. Reth, Annu. Rev. Immunol. 10, 97 (1992).
    • (1994) Immunol. Today , vol.15 , pp. 393
    • Pleiman, C.M.1    D'Ambrosio, D.2    Cambier, J.C.3
  • 3
    • 0027336583 scopus 로고
    • M. R. Gold and A. L. DeFranco, Adv. Immunol. 55, 221 (1994); C. M. Pleiman, D. D'Ambrosio, J. C. Cambier, Immunol. Today 15, 393 (1994); M. S. Neuberger et al., Immunol. Rev. 132, 147 (1993); M. Reth, Annu. Rev. Immunol. 10, 97 (1992).
    • (1993) Immunol. Rev. , vol.132 , pp. 147
    • Neuberger, M.S.1
  • 4
    • 0026750092 scopus 로고
    • M. R. Gold and A. L. DeFranco, Adv. Immunol. 55, 221 (1994); C. M. Pleiman, D. D'Ambrosio, J. C. Cambier, Immunol. Today 15, 393 (1994); M. S. Neuberger et al., Immunol. Rev. 132, 147 (1993); M. Reth, Annu. Rev. Immunol. 10, 97 (1992).
    • (1992) Annu. Rev. Immunol. , vol.10 , pp. 97
    • Reth, M.1
  • 5
    • 0027296743 scopus 로고
    • K. J. Patel and M. S. Neuberger, Cell 74, 939 (1993); A. Lanzavecchia, Curr. Opin. Immunol. 8, 348 (1996).
    • (1993) Cell , vol.74 , pp. 939
    • Patel, K.J.1    Neuberger, M.S.2
  • 8
    • 26544452008 scopus 로고    scopus 로고
    • in preparation
    • L domains of the NIP-specific antibody B1-8, followed by a CD8α linker region and the CD3ζ transmembrane part, and carries a Bam HI cloning site after the CD3ζ sequence. Bam HI fragments of the wild-type or mutated cytoplasmic γ2am and mb-1 coding sequences were obtained by PCR and inserted into psc-cyt to yield the expression vectors pANPCD8γ2am, pANPCD8γ2amY20L, pANPCD8γ2amY20L,M23L, pANPCD8mb-1, and pANPCD8mb-1M1. The pANPCD8tl vector encodes a scAgR with a short cytoplasmic sequence of the five amino acids RRIDP. The vectors were linearized with Pvu I and introduced into K46 and K46λ 12 cells by electroporation.
    • Müller, R.1    Reth, M.2
  • 10
    • 1842322595 scopus 로고    scopus 로고
    • note
    • Single-letter abbreviations for the amino acid residues are as follows: D, Asp; G, Gly; I, Ile; K, Lys; L, Leu; M, Met; N, Asn; P, Pro; R, Arg; S, Ser; V, Val; X, any amino acid; and Y, Tyr.
  • 11
    • 1842350422 scopus 로고    scopus 로고
    • note
    • Transfected cells were surface-biotinylated and lysed with 1% digitonin buffer as described (5). After a preclearing step with Sepharose beads, NIP-specific molecules were immunoprecipitated from the lysates with the use of NIP-Sepharose or Sepharose as control. After being washed three times with the 1% digitonin buffer, the NIP-Sepharose beads were boiled in reducing SDS sample buffer. The released proteins were separated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) on 10% gels and electroblotted onto a nitrocellulose membrane. Biotinylated proteins were detected with Str-HRP and the ECL detection system (Amersham, Braunschweig, Germany).
  • 12
    • 1842283354 scopus 로고    scopus 로고
    • note
    • 450. Mean values and standard deviations from triplicates were then used to plot the dose-response curves. The statistical relevance of each mean value was verificated by use of Student's t test. Apart from transfectant K46λγ2amY20L, two or three independent clones of each transfectant were analyzed. B cells were propagated in RPMI 1640 medium containing 10% fetal calf serum (FCS) ( Vitromex, Vilshofen, Germany) and G418 (0.5 mg/ml); the T cells were cultured in DMEM containing 10% FCS and, in case of the CTLL cells, 5% of supernatant of the IL-2-producing transfectant X63-IL-2.
  • 14
  • 19
    • 0026315047 scopus 로고
    • J. L. Goldstein, M. S. Brown, R. G. W. Anderson, D. W. Russel, W. J. Schneider, Annu. Rev. Cell Biol. 1, 1 (1985); A. Bansal and L. M. Gierasch, Cell 67, 1195 (1991); I. V. Sandoval and O. Bakke, Trends Cell Biol. 4, 292 (1994).
    • (1991) Cell , vol.67 , pp. 1195
    • Bansal, A.1    Gierasch, L.M.2
  • 20
    • 0028236274 scopus 로고
    • J. L. Goldstein, M. S. Brown, R. G. W. Anderson, D. W. Russel, W. J. Schneider, Annu. Rev. Cell Biol. 1, 1 (1985); A. Bansal and L. M. Gierasch, Cell 67, 1195 (1991); I. V. Sandoval and O. Bakke, Trends Cell Biol. 4, 292 (1994).
    • (1994) Trends Cell Biol. , vol.4 , pp. 292
    • Sandoval, I.V.1    Bakke, O.2
  • 22
    • 0028964696 scopus 로고
    • R. N. Mitchell et al., J. Exp. Med. 181, 1705 (1995); T. L. Stevens, J. H. Blum, S. P. Foy, L. Matsuuchi, A. L. DeFranco, J. Immunol. 152, 4397 (1994).
    • (1995) J. Exp. Med. , vol.181 , pp. 1705
    • Mitchell, R.N.1
  • 26
    • 0030994044 scopus 로고    scopus 로고
    • T. Kaisho, F. Schwenk, W. Müller, K. Rajewsky, Science 276, 412 (1997); G. Achatz and M. C. Lamers, ibid., p. 409.
    • Science , pp. 409
    • Achatz, G.1    Lamers, M.C.2
  • 27
    • 1842357395 scopus 로고    scopus 로고
    • note
    • We thank L. Nitschke, M. C. Lamers and K. Rajewsky for communication of unpublished data; T. Brocker, K. Karjalainen, and T. Simon for single-chain vectors; and L. Leclercq and P. Nielsen for critical reading of this manuscript. Supported partly by grants from the Deutsche Forschungsgemeinschaft (Re 571-3-1 and SFB 364).


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