Site-directed mutagenesis for large insertions by oligonucleotide primers in optimized molar ratios

BioTechniques

Volumn 22, Issue 1, 1997, Pages 70-72

  Wang, Dong ^a   Sul, Hei Sook ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

PLASMID DNA;

ARTICLE; DNA SYNTHESIS; ESCHERICHIA COLI; GENE EXPRESSION; GENE INSERTION; GENETIC TRANSFORMATION; NONHUMAN; PLASMID; SITE DIRECTED MUTAGENESIS;

EID: 1842372148     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/97221bm14     Document Type: Article

References (9)

1. Carter, P. 1987. Improved oligonucleotide-directed mutagenesis using M13 vectors. Methods Enzymol. 154:382-403.
2. Deng, W.P. and J.A. Nickoloff. 1992. Site-directed mutagenesis of virtually any plasmid by eliminating a unique site. Anal. Biochem. 200:81-88.
3. Hemsley, A., N. Arnheim, M.D. Toney, G. Cortopassi and D.J. Galas. 1989. A simple method for site-directed mutagenesis using the polymerase chain reaction. Nucleic Acids Res. 17:6545-6551.
4. Hughes, M.J.G. and D.W. Andrews. 1996. Creation of deletion, insertion and substitution mutations using a single pair of primers and PCR. BioTechniques 20:188-196.
5. Kunkel, T.A. 1985. Rapid and efficient site-specific mutagenesis without phenotypic selection. Proc. Natl. Acad. Sci. USA 82:488-492.
6. Lewis, M.K. and D.V. Thompson. 1990. Efficient site-directed in vitro mutagenesis using ampicillin selection. Nucleic Acids Res. 18:3439-3443.
7. Sambrook, J., E.F. Fritsch and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
8. Stemmer, W.P.C. and S.K. Morris. 1992. Enzymatic inverse PCR: a restriction site independent, single-fragment method for high-efficiency, site-directed mutagenesis. BioTechniques 13:214-220.
9. Tomic, M., I. Sunjevaric, E.S. Savtchenko and M. Blumenberg. 1990. A rapid and simple method for introducing specific mutations into any position of DNA leaving all other positions unaltered. Nucleic Acids Res. 18:1656.