Detection of sewage-derived Escherichia coli in a rural stream using multiplex PCR and automated DNA detection

Water Science and Technology

Volumn 35, Issue 11-12, 1997, Pages 337-342

  Turner, S J ^a   Lewis, G D ^a,b   Bellamy, A R ^a  

^a UNIVERSITY OF AUCKLAND   (New Zealand)

^b UNIVERSITY OF AUCKLAND   (New Zealand)

Author keywords

Animal; Escherichia coli; Faeces; Human; Molecular marker; RAPD; Water quality

Indexed keywords

BIOSYNTHESIS; COLIFORM BACTERIA; CONTAMINATION; DNA; EFFLUENTS; MANURES; SEWAGE; SURFACE WATERS; WATER POLLUTION CONTROL; WATER QUALITY; WATER TREATMENT PLANTS;

FAECES; POLYMERASE CHAIN REACTION; RANDOMLY AMPLIFIED POLYMORPHIC DNA MARKER; STREAM;

WATER BACTERIOLOGY;

CONFERENCE PAPER; ENVIRONMENTAL MONITORING; ESCHERICHIA COLI; POLYMERASE CHAIN REACTION; RANDOM AMPLIFIED POLYMORPHIC DNA; SEWAGE EFFLUENT; WATER CONTAMINATION; WATER QUALITY;

ANIMALIA; ESCHERICHIA COLI;

EID: 1842367310     PISSN: 02731223     EISSN: None     Source Type: Book Series    
DOI: 10.1016/S0273-1223(97)00282-5     Document Type: Conference Paper

References (4)

1. APHA (1992). Standard Methods for the Examination of Water and Wastewater, 17th edn., Washington DC, USA.
2. Fregeau, C. J. and Fourney, R. M. (1993). DNA typing with fluorescently tagged short tandem repeats: A sensitive and accurate approach to human identification. BioTechniques, 15, 100-119.
3. Turner, S. J., Bellamy, A. R. and Lewis G. D. (1996). Identification of markers for distinguishing E. coli from human and non-human sources using Randomly Amplified Polymorphic DNA analysis (in preparation).
4. Ziegle, J. S., Su, Y., Corcoran, K. P., Nie, L., Mayrand, P. E., Hoff, L. B., McBride, L. J., Kronick, M. L. and Diehl, S. R. (1992). Application of Automated DNA sizing technology for genotyping microsatellite loci. Genomics, 14, 1027-1031.