Functional analysis of pneumolysin by use of monoclonal antibodies

Infection and Immunity

Volumn 64, Issue 2, 1996, Pages 480-484

  De Los Toyos, Juan R ^a   Méndez, Francisco J ^a   Aparicio, Jesús F ^a   Vázquez, Fernando ^a   Suárez, Maria Del Mar García ^a   Fleites, Ana ^a   Hardisson, Carlos ^a   Morgan, Peter J ^b   Andrew, Peter W ^b   Mitchell, Timothy J ^b  

^a UNIVERSIDAD DE OVIEDO   (Spain)

^b UNIVERSITY OF LEICESTER   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL TOXIN; EPITOPE; MONOCLONAL ANTIBODY; PNEUMOLYSIN;

ANIMAL CELL; ANIMAL EXPERIMENT; ANTIBODY PRODUCTION; ARTICLE; BACTEREMIA; BACTERIAL VIRULENCE; CONTROLLED STUDY; FEMALE; MALE; MEMBRANE DAMAGE; MOUSE; NONHUMAN; PRIORITY JOURNAL; RESPIRATORY BURST; STREPTOCOCCUS PNEUMONIAE; TOXIN ANALYSIS;

EID: 1842323092     PISSN: 00199567     EISSN: None     Source Type: Journal    
DOI: 10.1128/iai.64.2.480-484.1996     Document Type: Article

References (19)

1. Alexander, J. E., R. A. Lock, C. C. A. M. Peeters, J. T. Poolman, P. W. Andrew, T. J. Mitchell, D. Hansman, and J. C. Paton. 1994. Immunization of mice with pneumolysin toxoid confers a significant degree of protection against at least nine serotypes of Streptococcus pneumoniae Infect. Immun. 62:5683-5688.
2. Berry, A. M., J. Yother, D. E. Briles, D. Hansman, and J. C. Paton. 1989. Reduced virulence of a defined pneumolysm-negative mutant of Streptococcus prieumoniae. Infect. Immun. 57:2037-2042.
3. Boulnois, G. J., J. C. Paton, T. J. Mitchell, and P. W. Andrew. 1991. Structure and function of pneumolysin, the multifunctional, thiol-activated toxin of Streptococcus pneumoniae. Mol. Microbiol. 5:2611-2616.
4. Canvin, J. R., A. P. Marvin, M. Sivakumaran, J. C. Paton, G. J. Boulnois, P. W. Andrew, and T. J. Mitchell. 1995. The role of pneumolysin and autolysin in the pathology of pneumonia and septicemia in mice infected with a type 2 pneumococcus. J Infect. Dis. 172:119-123.
5. Dick, T. B., and C. G. Gemmell. 1971. The pathogenesis of pneumococcal infection in mice. J. Med. Microbiol. 4:153-163.
6. Hugo, F., J. Reichwein, M. Arvand, S. Krämer, and S. Bhakdi. 1986. Use of a monoclonal antibody to determine the mode of transmembrane pore formation by streptolysin O. Infect. Immun. 54:641-645.
7. Jameson, B. A., and H. Wolf. 1988. The antigenic index: a novel algorithm for predicting antigenic determinants. Comput. Appl. Biosci. 4:181-186.
8. Liddell, J. E., and A. Cryer. 1991. A practical guide to monoclonal antibodies. John Wiley, Chichester, United Kingdom.
9. Mitchell, T. J., J. A. Walker, F. K. Saunders, P. A. Andrew, and G. J. Boulnois. 1989. Expression of the pneumolysin gene in Escherichia coli rapid purification and biological properties. Biochim. Biophys. Acta 1007:67-72.
10. Morgan, P. J., S. C. Hyman, A. J. Rowe, T. J. Mitchell, P. W. Andrew, and H. R. Sabil. 1995. Subunit organisation and symmetry of pore-forming oligomeric pneumolysin. FEBS Lett. 371:77-80.
11. Nato, F., K. Reich, S. Lhopital, S. Ronyre, C. Geoffroy, J. C. Mazie, and P. Cossart. 1991. Production and characterization of neutralizing and nonneu-tralizing monoclonal antibodies against listeriolysin O. Infect. Immun. 59: 4641-4646.
12. Nutman, T. B. 1991. Measurement of polyclonal immunoglobulin synthesis using ELISA. p. 7.12.1-7 12 6. In J. E. Coligan et al. (ed.). Current protocols in immunology. John Wiley & Sons, New York
13. Owen, R. H. G. O., G. J. Boulnois, P. W. Andrew, and T. J. Mitchell. 1994 A role in cell-binding for the C-terminus of pneumolysin, the thiol-activated toxin of Streptococcus pneumoniae. FEMS Microbiol Lett. 121:217-222.
14. Paton, J. C., P. W. Andrew, G. J. Boulnois, and T. J. Mitchell. 1993. Molecular analysis of the pathogenicity of Streptococcus pneumoniae: the role of pneumococcal proteins. Annu. Rev. Microbiol 47:89-115.
15. Rath, S., C. M. Stanley, and M. W. Steward. 1988. An inhibition enzyme immunoassay for estimating relative antibody affinity and affinity heterogeneity J. Immunol Methods 106:245-249
16. Rubins, J. B., D. Charboneau, J. C. Paton, T. J. Mitchell, P. W. Andrew, and E. N. Janoff. 1995. Dual function of pneumolysin in the early pathogenesis of murine pneumococcal pneumonia. J. Clin. Invest. 95:142-150.
17. Salo, P., A. Närvänen, and M. Leinonen. 1993. Mapping of immunoreactive sites of pneumococcal pneumolysin by use of synthetic peptides Infect. Immun 61:2822-2826.
18. Smyth, C. J., and J. L. Duncan. 1978. Thiol-activated (oxygen-labile) cytolysins, p. 129-183. In 1. Jeljaszewicz and T. Wadstrom (ed.), Bacterial toxins and cell membranes. Academic Press. London.
19. Tuomanen, E. L., R. Austrian, and H. R. Masure. 1995 Pathogenesis of pneumococcal infection N. Engl. J Med. 332:1280-1284