Improved method for the construction of cosmid sublibraries from yeast artificial chromosomes

BioTechniques

Volumn 22, Issue 2, 1997, Pages 264-268

  Müller, Hans Werner ^a   Meese, Eckart U ^a  

^a SAARLAND UNIVERSITY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

DNA FRAGMENT;

ARTICLE; CHROMOSOME ANALYSIS; COSMID; DNA DETERMINATION; ELECTROPHORESIS; HYBRIDIZATION; MOLECULAR CLONING; YEAST;

EID: 1842296990     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/97222bm14     Document Type: Article

References (7)

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2. Buckler, A.J., D.D. Chan, S.L. Graw, D. Brook, D.A. Haber, P.A. Sharp and D.E. Housman. 1991. Exon amplification: a strategy to isolate mammalian genes based on RNA splicing. Proc. Natl. Acad. Sci. USA 88:4005-4009.
3. Fischer U., H.-W. Mueller, H.P. Sattler, K. Feiden, K.D. Zang and E. Meese. 1995. Amplification of the MET gene in glioma. Brief communication. Genes Chromosom. Cancer 12:63-65.
4. Larsen, F., G. Gundersen, R. Lopez and H. Prydz. 1992. CpG islands as gene markers in the human genome. Genomics 13:1095-1107.
5. Lovett, M., J. Kere and L.M. Hinton. 1991. Direct selection: a method for the isolation of cDNAs encoded by large genomic regions. Proc. Natl. Acad. Sci. USA 88:9628-9632.
6. Stratagene. 1993. SuperCos1 Cosmid Vector Kit Instruction Manual. La Jolla, CA.
7. Trofatter J.A., M.M. MacCollin, J.L. Rutter, J.R. Murrell, M.P. Duyao, D.M. Parry, R. Eldrige, N. Kley, A.G. Menon, K. Pulaski et al. 1993. A novel moesin-ezrin- radixin-like gene is a candidate for neurofibromatosis 2 tumor suppressor. Cell 72:791-800.