Use of a single sequencing termination reaction to distinguish between cytosine and 5-methylcytosine in bisulfite-modified DNA

BioTechniques

Volumn 22, Issue 5, 1997, Pages 850-854

  Zhou, Yuanxiang ^a   Magill, Jane M ^a   Newton, R J ^a   Magill, Clint ^a  

^a TEXAS A AND M UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

5 METHYLCYTOSINE; BISULFITE; CYTOSINE;

ARTICLE; DNA DEGRADATION; DNA DETERMINATION; DNA METHYLATION; NUCLEOTIDE SEQUENCE;

EID: 1842292179     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/97225bm14     Document Type: Article

References (5)

1. Frommer, M., L.E. McDonald, D.S. Miller, C.M. Collis, F. Watt, G.W. Grigg, P.L. Molloy and C.L. Paul. 1992. A genomic sequencing protocol that yields a positive display of 5-methylcytosine residues in individual DNA strands. Proc. Natl. Acad. Sci. USA 89:1827-1831.
2. Meyer, P., I. Niedenhof and M. Ten Lohuis. 1994. Evidence for cytosine methylation of non-symmetrical sequences in transgenic Petunia hybrida. EMBO J. 13:2084-2088.
3. Selker, E.U., D.Y. Fritz and M.J. Singer. 1993. Dense nonsymmetrical DNA methylation resulting from repeat-induced point mutation in Neurospora. Science 262:1724-1728.
4. Shapiro, R., B. Braverman, J.B. Louis and R.E. Servis. 1973. Nucleic acid reactivity and conformation. II. Reaction of cytosine and uracil with sodium bisulfite. J. Biol. Chem. 248:4060-4064.
5. Wang, R.Y.-H., C.W. Gehrke and M. Ehrlich. 1980. Comparison of bisulfite modification of 5-methyldeoxycytidine and deoxycytidine residues. Nucleic Acids Res. 8:4777-4790.