Evidence that GCN1 and GCN20, translational regulators of GCN4, function on elongating ribosomes in activation of eIF2α kinase GCN2

Molecular and Cellular Biology

Volumn 17, Issue 8, 1997, Pages 4474-4489

  Marton, Matthew J ^a,c   Vazquez De Aldana, Carlos R ^a,d   Qiu, Hongfang ^a   Chakraburtty, Kalpana ^b   Hinnebusch, Alan G ^a  

^a EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH AND HUMAN DEVELOPMENT   (United States)

^b MEDICAL COLLEGE OF WISCONSIN   (United States)

^c Fred Hutchinson Cancer Research Center   (United States)

^d UNIVERSITY OF SALAMANCA   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

ELONGATION FACTOR 3; INITIATION FACTOR 2; PROTEIN KINASE; REGULATOR PROTEIN; RIBOSOME INACTIVATING PROTEIN;

ARTICLE; ENZYME ACTIVATION; IMMUNOFLUORESCENCE; MATHEMATICAL MODEL; MEMBRANE BINDING; PRIORITY JOURNAL; REGULATOR GENE; SACCHAROMYCES CEREVISIAE; SEQUENCE HOMOLOGY; TRANSCRIPTION REGULATION; TRANSLATION REGULATION;

SACCHAROMYCES CEREVISIAE;

EID: 1842287951     PISSN: 02707306     EISSN: None     Source Type: Journal    
DOI: 10.1128/mcb.17.8.4474     Document Type: Article

References (54)

1. Alani, E., L. Cao, and N. Kleckner. 1987. A method for scne disruption that allows repeated use of URA3 selection in the construction of multiply disrupted yeast strains. Genetics 116:541-545.
2. Altschul, S. F., W. Gish, W. Miller, E. W. Myers, and D. J. Lipman. 1990. Basic local alignment search tool. J. Mol. Biol. 215:403-410.
3. Anderson, J. T., M. R. Paddy, and M. S. Swanson. 1993. PUB1 is a major nuclear and cytoplasmic polyadenylated RNA-binding protein in Saccharomyces cerevisiae. Mol. Cell. Biol. 13:6102-5113.
4. Belfield, G. P., N. J. Ross-Smith, and M. F. Tuite. 1995. Translation elongation factor-3 (EF-3): an evolvine eukaryotic ribosomal protein. J. Mol. Evol. 41:376-387.
5. Berkower, C., and S. Michaelis. 1991. Mutational analysis of the yeast a-factor transporter STE6, a member of the ATP binding cassette (ABC) protein superfamily. EMBO J. 10:3777-3785.
6. Boeke, J. D., F. LaCroute, and G. R. Fink. 1984. A positive selection for mutants lacking orotidinc-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance. Mol. Gen. Genet. 197:345-346.
7. Boguski, M. S., and F. McCormick. 1993. Proteins regulating Ras and its relatives. Nature 366:643-654.
8. Cashel, M., and K. E. Rudd. 1987. The stringent response, p. 1410-1438. In F. C. Neidhardt, J. L. Ingraham, K. B. Low, B. Magasanik, M. Schaechter, and H. E. Umbarger (ed.), Escherichia coli and Salmonella typhimurium: cellular and molecular biology. American Society for Microbiology, Washington. D.C.
9. Cutting, G. R., L. M. Kasch, B. J. Rosentein, J. Zielenski, L.-C. Tsui, S. E. Antonarakis, and H. Kazazian. 1990. A cluster of cystic fibrosis mutations in the first nucleotide-binding fold of the cystic fibrosis conductance regulator protein. Nature 346:366-369.
10. Dasmahapatra, B., and K. Chakraburttry. 1981. Protein synthesis in yeast. I. Purification and properties of elongation factor 3 from Saccharomyces cerevisiae. J. Biol. Chem. 256:9999-10004.
11. Dever, T. E., L. Feng, R. C. Wek, A. M. Cigan, T. D. Donahue, and A. G. Hinnebusch. 1992. Phosphorylation of initiation factor 2α by protein kinase GCN2 mediates gene-specific translational control of GCN4 in yeast. Cell 68:585-596.
12. Devereux, J., P. Haeberli, and O. Smithies. 1984. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 12:387-395.
13. Dunahue, T. F., R. S. Daves, G. Lucchini, and G. R. Fink. 1983. A short nucleotide sequence required for regulation of HIS4 by the general control system of yeast. Cell 32:89-98.
14. Durfee, T., K. Becherer, P. L. Chen, S. H. Yeh, Y. Yang, A. E. Kilburn, W. H. Lee, and S. J. Elledge. 1993. The retinoblastoma protein associates with the protein phosphatase type 1 catalytic subunit. Genes Dev. 7:555-569.
15. Fath, M. J., and R. Kolter. 1993. ABC transporters: bacterial exporters. Microbiol. Rev. 57:995-1017.
16. Feilotter, H. E., G. J. Hannon, C. J. Ruddell, and D. Beach. 1994. Construction of an improved host strain for two hybrid screening. Nucleic Acids Res. 22:15102-1503.
17. Feinberg, B., C. S. McLaughlin, and K. Moldave. 1982. Analysis of temperature-sensitive mutant ts187 of Succharomyces cerevisiae altered in a component required for the initiation of protein synthesis. J. Biol. Chem. 257: 1084-10851.
18. Ferro-Luzzi Ames, G. 1992. Bacterial periplasmic permeases as model systems for the superfamily of traffic ATPases, including the multidrug resistance protein and the cystic fibrosis transmembrane conductance regulator. Int. Rev. Cytol. 137A: 1-35.
19. Foiani, M., A. M. Cigan, C. J. Paddon, S. Harashima, and A. G. Hinnebusch. 1991. GCD2, a translational repressor of the GCN4 gene, has a general function in the initiation of protein synthesis in Saccharomyces cerevisiae. Mol. Cell. Biol. 11:3203-3216.
20. Gietz, R. D., and A. Sugino. 1988. New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites. Gene 74:527-34.
21. Hannig, E. H., N. P. Williams, R. C. Wek, and A. G. Hinnebusch. 1990. The translational activator GCN3 functions downstream from GCN1 and GCN2 in the regulatory pathway that couples GCN4 expression to amino acid availability in Saccharomyces-fs cerevisiae. Genetics 126:549-562.
22. Hershey, J. W. B. 1991. Translational control in mammalian cells. Annu. Rev. Biochem. 60:717-755.
23. Higgins, C. F. 1992. ABC transporters: from microorganisms to man. Annu. Rev. Cell Biol. 8:67-113.
24. Hinnebusch, A. G. 1996. Translational control of GCN4: gene-specific regulation by phosphorylation of eIF2, p. 199-244. In J. W. B. Hershey, M. B. Malhews, and N. Sonenberg (ed.), Translational control. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
25. Kamath, A., and K. Chakraburtty. 1989. Role of yeast elongation factor 3 in the elongation cycle. J. Biol. Chem. 264:15423-15428.
26. +-ATPase. J. Biol. Chem. 267:447-154.
27. Klionsky, D. J., P. Herman, and S. D. Emr. 1990. The fungal vacuole: composition, function, and biogenesis. Microbiol. Rev. 54:266-292.
28. Koerner, T. J., J. E. Hill, A. M. Myers, and A. Tzagoloff, 1991. Highexpression vectors with multiple cloning sites for construction of trpE fusion genes: pATH vectors. Methods Enzymol. 194:477-490.
29. Lanker, S., J. L. Bushman, A. G. Hinnebusch, H. Trachsel, and P. P. Mueller. 1992. Autoregulation of the yeast lysyl-tRNA synthetase gene GCD5/KRS1 by translational and tianscriptional control mechanisms. Cell 70:647-657.
30. Lennon, G., C. Auffray, M. Pnlymeropoulns, and M. B. Soares. 1996. The I.M.A.G.E. consortium: an integrated molecular analysis of genomes and their expression. Genomics 33:151-152.
31. Marton, M. J., D. Crouch, and A. G. Hinnebusch. 1993. GCN1, a translational activator of GCN4 in Saccharomyces cerevisiae, required for phosphorylalion of eukaryotic translation initiation factor 2 by protein kinase GCN2. Mol. Cell. Biol. 13:3541-3556.
32. Marton, M. J., and A. G. Hinnebusch. Unpublished data.
33. Naranda, T., S. E. MacMillan, and J. W. B. Hershey. 1994. Purified yeast translational initiation factor eIF-3 is an RNA-binding protein complex that contains the PRT1 protein. J. Biol. Chem. 269:32286-32292.
34. Nichols, C. G., S.-L. Shyng, A. Nestorowicz, B. Glaser, J. P. Clement, G. Gonzalez, L. Aguilar-Bryan, M. A. Permutt, and J. Bryan. 1996. Adenosine diphosphate as an intracellular regulator ot insulin secretion. Science 272: 1785-1787.
35. Petitjean, A., N. Bonneuaud, and F. Lacroute. 1995. The duplicated Saccharomyces cerevisiae gene SSM1 encodes a eucaryotic homolog of the eubacterial and archaebacterial L1 ribosomal proteins. Mol. Cell. Biol. 15:5071-5081.
36. Ramirez, M., R. C. Wek, and A. G. Hinnebusch. 1991. Ribosome-association of GCN2 protein kinase, a translational activator of the GCN4 gene of Saccharomyces cerevisiae. Mol. Cell. Biol. 11:3027-3036.
37. Richter, D. 1976. Stringent factor from Escherichia culi directs ribosomal binding and release of uncharged tRNA. Proc. Natl. Acad. Sci. USA 73:707-711.
38. Roberts, C. J., C. K. Raymond, C. T. Yamashiro, and T. H. Stevens. 1991. Methods for studying the yeast vacuole. Methods Enzymol. 194:644-661.
39. Rolfes, R. J., F. Zhang, and A. G. Hinnebusch. 1997. The transcriptional activators BAS1, BAS2, and ABF1 bind positive regulatory sites as the critical elements for adenine regulation of ADE5,7. J. Biol. Chem. 272: 13343-13354.
40. Sandbaken, M., J. A. Lupisella, B. DiDomenico, and K. Chakraburtty. 1990. Isolation and characterization of the structural gene encoding elongation factor 3. Biochim. Biophys. Acta 1050:230-234.
41. Sato, T., Y. Ohsumi, and Y. Anraku. 1984. An arginine/histidine exchange transport system in vacuolar-membrane vesicles of Saccharomyces cerevisiae. J. Biol. Chem. 259:11509-11511.
42. Sato, T., Y. Ohsumi, and Y. Anraku. 1984. Substrate specificities of active transport systems for amino acids in vacuolar-membrane vesicles of Saccharomyces cerevisiae. J. Biol. Chem. 259:11505-11508.
43. Sikorski, R. S., and P. Mieter. 1989. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122:19-27.
44. Skogerson, L., and E. Wakatama. 1976. A ribosome-dependent GTPase from yeast distinct from elongation factor 2. Proc. Natl. Acad. Sci. USA 73:73-76.
45. Szczypka, M. S., J. A. Wemmie, W. S. Moye-Rowley, and D. J. Thiele. 1994. A yeast metal resistance protein similar to human cystic fibrosis transmembrane conductance regulator (CFTR) and multidrug resistance-associated protein. J. Biol. Chem. 269:22853-22857.
46. Triana-Alonso, F. J., K. Chakraburtty, and K. H. Nierhaus. 1995. The elongation factor unique in higher fungi and essential for protein biosynthesis is an E site factor. J. Biol. Chem. 270:20473-20478.
47. Uritani, M., and M. Miyazaki. 1988. Role of yeast elongation factor 3 (EF-3) at the AA-tRNA binding step. J. Biochem. 104:118-126.
48. Vazquez de Aldana, C. R., M. J. Marton, and A. G. Hinnebusch. 1995. GCN20, a novel ATP binding cassette protein, and GCN1 reside in a complex that mediates activation of the eIF-2u kinase GCN2 in amino acidstarved cells. EMBO J. 14:3184-3199.
49. Wek, R. C., B. M. Jackson, and A. G. Hinnebusch. 1989 Juxtaposition of domains homologous to protein kinases and histidyl-tRNA synthetases in GCN2 protein suggests a mechanism for coupling GCN4 expression to amino acid availability. Proc. Natl. Acad. Sci. USA 86:4579-1583.
50. Wek, S. A., S. Zhu, and R. C. Wek. 1995. The histidyl-tRNA synthetaserelated sequence in the eIF-2α protein kinase GCN2 interacts with tRNA and is required for activation in response to starvation for different amino acids. Mol. Cell. Biol. 15:4497-4506.
51. Wemmie, J. A., M. S. Szczypka, D. J. Thiele, and W. S. Moye-Rowley. 1994. Cadmium tolerance mediated by the yeast AP-1 protein requires the presence of an ATP-binding cassette transporter-encoding gene. YCF1. J. Biol. Chem. 269:32592-32597.
52. Wiemkin, A., M. Schellenberg, and K. Urech. 1979. Vacuoles: the sole compartments of digestive enzymes in yeast Saccharomyces cerevisiae? Arch. Microbiol. 123:23-35.
53. Yamashiro, C. T., P. M. Kane, D. F. Wolczyk, R. A. Preston, and T. H. Stevens. 1990. Role of vacuolar acidification in protein sorting and zymogen activation: a genetic analysis of the yeast vacuolar proton-translocating ATPase. Mol. Cell. Biol. 10:3737-3749.
54. Yon, J., and M. Fried. 1988. Precise gene fusion by PCR. Nucleic Acids Res. 17:4895.