Use of flow field-flow fractionation for the rapid quantitation of ribosome and ribosomal subunits in Escherichia coli at different protein production conditions

Biotechnology and Bioengineering

Volumn 54, Issue 5, 1997, Pages 461-467

  Nilsson, Mikael ^a   Bülow, Leif ^a   Wahlund, Karl Gustav ^a  

^a LUND UNIVERSITY   (Sweden)

Author keywords

flow field flow fractionation; quantitation; ribosomes; subunits

Indexed keywords

BACTERIA; BIOSYNTHESIS; CELLS; CENTRIFUGATION; COMPOSITION; FRACTIONATION; PROTEINS;

ESCHERICHIA COLI; FLOW FIELD FLOW FRACTIONATION; QUANTITATION; RIBOSOMES; SUBUNITS;

BIOTECHNOLOGY;

CELL PROTEIN; CHLORAMPHENICOL;

ANALYTIC METHOD; ARTICLE; BACTERIAL GROWTH; CONTROLLED STUDY; ESCHERICHIA COLI; FRACTIONATION; NONHUMAN; PLASMID; PROTEIN SYNTHESIS; RIBOSOME; RIBOSOME SUBUNIT;

ESCHERICHIA COLI;

EID: 18344417541     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(19970605)54:5<461::AID-BIT6>3.0.CO;2-C     Document Type: Article

References (16)

1. Birnbaum, S., Bailey, J. E. 1991. Plasmid presence changes the relative levels of many host cell proteins and ribosome components in recombinant Escherichia coli. Biotechnol. Bioeng. 37: 736-745.
2. Bremer, H., Dennis, P. P. 1987. Modulation of chemical composition and other parameters of the cell by growth rate, pp. 1527-1542. In: F. C. Neidhardt, J. L. Ingraham, K. B. Low, B. Magasanik, M. Schaechter, and H. E. Umbarger (eds.), Escherichia coli and Salmonella typhimurium. ASM, Washington, DC.
3. 4/lactate dehydrogenase facilitate decarboxylation of oxaloacetate. Prot. Eng. 6: 907-911.
4. Giddings, J. C. 1993. Field-flow fractionation: Analysis of macromolecular, colloidal, and particulate materials. Science 260: 1456-1465.
5. Hauge, J. G. 1971. Pressure-induced dissociation of ribosomes during ultracentrifugation. FEBS Lett. 17: 168-172.
6. Jinks-Robertson, S., Nomura, M. 1987. Ribosomes and tRNA, pp. 1358-1385. In: F. C. Neidhardt, J. L. Ingraham, K. B. Low, B. Magasanik, M. Schaechter, and H. E. Umbarger (eds.), Escherichia coli and Salmonella typhimurium. ASM, Washington, DC.
7. Litzén, A. 1993. Separation speed, retention, and dispersion in asymmetrical flow field-flow fractionation as functions of channel dimensions and flow rates. Anal. Chem. 65: 461-470.
8. Liveris, D., Klotsky, R.-A., Schwartz, I. 1991. Growth rate regulation of translation initiation factor IF3 biosynthesis in Escherichia coli. J. Bact. 173: 3888-3893.
9. Maaløe, O. 1979. Regulation of the protein-synthesizing machinery -ribosomes, tRNA, factors, and so on, pp. 487-542. In: R. F. Golberger (ed.), Biological regulation and development, vol. 1. Plenum, New York.
10. Mendelsohn, J., Tissières, A. 1959. Variations in the amount of ribonucleoprotein particles in Escherichia coli. Biochim. Biophys. Acta 35: 248-250.
11. Nilsson, M., Birnbaum, S., Wahlund, K.-G. 1996. Determination of relative amounts of ribosome and subunits in Escherichia coli using asymmetrical flow field-flow fractionation. J. Biochem. Biophys. Methods 33: 9-23.
12. Pettersson, I., Hardy, S. J. S., Liljas, A. 1976. The ribosomal protein L8 is a complex of L7/L12 and L10. FEBS Lett. 64: 135-138.
13. Spirin, A. S. 1971. On the equilibrium of the association-dissociation reaction of ribosomal subparticles and on the existence of the socalled "60 S intermediate" ("swollen 70 S") during centrifugation of the equilibrium mixture. FEBS Lett. 14: 349-353.
14. Spirin, A. S., Sofronova, M. Yu., Sabo, B. 1970. Dissociation of 70 S monoribosomes of Escherichia coli in the presence of a reduced medium magnesium ion concentration. Mol. Biol. 4: 501-508.
15. Vasquez, D. 1964. The binding of chloramphenicol by ribosomes from Bacillus megaterium. Biochem. Biophys. Res. Commun. 15: 464-468.
16. Wittgren, B., Wahlund, K.-G., Dérand, H., Wesslén. B. 1996. Aggregation behavior of an amphiphilic graft copolymer in aqueous medium studied by asymmetrical flow field-flow fractionation. Macromolecules 29: 268-276.