Uncoupling of protein-3 induces an uncontrolled uncoupling of mitochondria after expression in muscle derived L6 cells

European Journal of Biochemistry

Volumn 269, Issue 5, 2002, Pages 1373-1381

  Guerini, Danilo ^a   Prati, Elisabetta ^a   Desai, Urvi ^a   Nick, Hans Peter ^a   Flammer, Rolf ^a   Grüninger, Stephan ^a   Cumin, Frederic ^a   Kaleko, Machael ^a   Connelly, Sheila ^a   Chiesi, Michele ^a  

^a NOVARTIS PHARMA AG   (Switzerland)

Author keywords

Adenovirus; Mitochondria; Respiration; Thermogenesis; Uncoupling protein

Indexed keywords

ADENOVIRUS VECTOR; FATTY ACID; PURINE NUCLEOTIDE; TRITON X 100; UNCOUPLING PROTEIN 3;

ANIMAL CELL; ARTICLE; CELL LINE; CONTROLLED STUDY; IMMUNOFLUORESCENCE MICROSCOPY; MAMMAL; MITOCHONDRION; MYOBLAST; NONHUMAN; OXYGEN CONSUMPTION; PRIORITY JOURNAL; PROTEIN EXPRESSION;

ANIMALS; CARRIER PROTEINS; CELLS, CULTURED; ION CHANNELS; MEMBRANE PROTEINS; MEMBRANE TRANSPORT PROTEINS; MITOCHONDRIA, MUSCLE; MITOCHONDRIAL PROTEINS; PROTEIN FOLDING; PROTEINS; RATS;

ADENOVIRIDAE; ANIMALIA; MAMMALIA;

EID: 18344387133     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1033.2002.02773.x     Document Type: Article

References (39)

1. Uncoupling protein-2: A novel gene linked to obesity and hyperinsulinemia
2. Cloning and characterization of an uncoupling protein homolog: A potential molecular mediator of human thermogenesis
3. Uncoupling protein-2 negatively regulates insulin secretion and is a major link between obesity, beta cell dysfunction, and type 2 diabetes
4. Increased uncoupling protein-2 levels in beta-cells are associated with impaired glucose-stimulated insulin secretion: Mechanism of action
5. Induction of UCP2 gene expression by LPS: A potential mechanism for increased thermogenesis during infection
6. A role for uncoupling protein-2 as a regulator of mitochondrial hydrogen peroxide generation
7. Uncoupling protein-3: A new member of the mitochondrial carrier family with tissue-specific expression
8. UCP3: An uncoupling protein homologue expressed preferentially and abundantly in skeletal muscle and brown adipose tissue
9. Cellular energy utilization and molecular origin of standard metabolic rate in mammals
10. Uncoupling proteins: Their roles in adaptive thermogenesis and substrate metabolism reconsidered
11. Recombinant human uncoupling protein-3 increases thermogenesis in yeast cells
12. Assessment of uncoupling activity of uncoupling protein 3 using a yeast heterologous expression system
13. Properties of the human long and short isoforms of the uncoupling protein-3 expressed in yeast cells
14. Uncoupling proteins 1 and 3 are regulated differently
15. Uncoupling protein-3 expression in rodent skeletal muscle is modulated by food intake but not by changes in environmental temperature
16. Uncoupling protein 3 (UCP3) stimulates glucose uptake in muscle cells through a phosphoinositide 3-kinase-dependent mechanism
17. Effects of adenoviral overexpression of uncoupling protein-2 and -3 on mitochondrial respiration in insulinoma cells
18. Transport function and regulation of mitochondrial uncoupling proteins 2 and 3
19. Uncoupling proteins 2 and 3 are highly active H(+) transporters and highly nucleotide sensitive when activated by coenzyme Q (ubiquinone)
20. Lack of obesity and normal response to fasting and thyroid hormone in mice lacking uncoupling protein-3
21. Energy metabolism in uncoupling protein 3 gene knockout mice
22. In vivo effects of uncoupling protein-3 gene disruption on mitochondrial energy metabolism
23. Mice overexpressing human uncoupling protein-3 in skeletal muscle are hyperphagic and lean
24. Physiological levels of mammalian uncoupling protein 2 do not uncouple yeast mitochondria
25. The bulk of UCP3 expressed in yeast cells is incompetent for a nucleotide regulated H+ transport
26. Phenotypic correction of diabetic mice by adenovirus-mediated glucokinase expression
27. Site-specific DNA recombination in mammalian cells by the Cre recombinase of bacteriophage P1
28. Elimination of both E1 and E2 from adenovirus vectors further improves prospects for in vivo human gene therapy
29. Evaluation of the concentration and bioactivity of adenovirus vectors for gene therapy
30. Retention of differentiation potentialities during prolonged cultivation of myogenic cells
31. UCP3 expressed in yeast is primarily localized in extramitochondrial particles
32. A water soluble luminescence oxygen sensor
33. Nature of the masking of nucleotide-binding sites in brown adipose tissue mitochondria. Involvement of endogenous adenosine triphosphate
34. Cysteine residues are not essential for uncoupling protein function
35. Assessment of uncoupling activity of the human uncoupling protein 3 short form and three mutants of the uncoupling protein gene using a yeast heterologous expression system
36. A mitochondrial uncoupling artifact can be caused by expression of uncoupling protein 1 in yeast
37. Uncoupling proteins 2 and 3 interact with members of the 14.3.3 family
38. Superoxide activates mitochondrial uncoupling proteins
39. The basal proton conductance of skeletal muscle mitochondria from transgenic mice overexpressing or lacking uncoupling protein-3