Protein engineering aspartic proteinases: Site-directed mutagenesis, biochemical characterisation, and X-ray analysis of chymosins with substituted single amino acid substitutions and loop replacements

Advances in Experimental Medicine and Biology

Volumn 436, Issue , 1998, Pages 169-177

  Albert, Armando ^a,b   Blundell, Tom L ^a,b   Dhanaraj, V ^a,b   Donate, Luis Enrique ^a,b   Groves, Matthew ^a   Guruprasad, Kunchur ^a,b   Nugent, Phil G ^a   Orprayoon, P ^a   Pitts, Jim E ^a   Rufino, Stephen ^a   Srinivasan, N ^a,b   Williams, Mark ^a,b   Wilsher, Julie ^a  

^a UNIVERSITY OF LONDON   (United Kingdom)

^b UNIVERSITY OF CAMBRIDGE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ASPARTIC PROTEINASE; CHYMOSIN;

AMINO ACID SUBSTITUTION; CATALYSIS; CONFERENCE PAPER; ENZYME ACTIVITY; GENETIC ENGINEERING; PH; PRIORITY JOURNAL; PROTEIN STRUCTURE; SITE DIRECTED MUTAGENESIS; THERMOSTABILITY; X RAY ANALYSIS;

AMINO ACIDS; ANIMALS; CHYMOSIN; CRYSTALLOGRAPHY, X-RAY; GENE EXPRESSION; MUTAGENESIS, SITE-DIRECTED; PROTEIN ENGINEERING; PROTEIN STRUCTURE, SECONDARY; RECOMBINANT FUSION PROTEINS;

EID: 18244411360     PISSN: 00652598     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4615-5373-1_23     Document Type: Article

References (19)

1. Kostka, V. (1985) Aspartic Proteinases and Their Inhibitors. P 613, Walter de Gruyer, Berlin.
2. Mantafounis, D. and Pitts, J.E. (1989). Biochem.Soc.Trans., 17, 763-764.
3. Strop, P., Sedlacek, J., Stys, J., Kaderabkova, Z., Blaha, I., Pavlickova, L., Pohl, J., Fabry, M., Kostka, V., Newman, M., Frazao, C., Shearer, A., Tickle, I.J. and Blundell, T.L. (1990) Biochemistry 29, 9863-9871.
4. Gilliland, G.L., Winbourne, E.L., Nachman, J. and Wlodawer, A. (1990). Proteins, 8, 82-101.
5. Newman, M., Safro, M., Frazao, C., Khan, G., Zdanov, A., Tickle, I.J., Blundell, T.L. and Andreeva, N. (1991). J.Mol.Biol., 221, 1295-1309.
6. Groves, M., Dhanaraj, V., Badasso, M., Pitts, J.E., Hoover, D.J. and Blundell, T.L. (1997). Prot.Engg., Submitted.
7. Topham, C.M., Srinivasan, N. and Blundell, T.L. (1997) Prot.Engg. 10, 7-21.
8. Park, Y.-N., Aikawa, J.-i., Nishiyama, M., Horinouchi, S. and Beppu, T. (1996). Prot.Engg. 9, 869-875.
9. Sutcliffe, M.J., Hannef, I. and Carney, D. and Blundell, T.L. (1987) Prot.Engg. 1, 377-384.
10. Sali, A. and Blundell, T.L. (1990) J.Mol.Biol. 212, 403-428.
11. Carney, D., Gardner, S., Hayes, F., Howlin, B., Hubbard, T., Overington, J., Singh, D., Sibanda, B.L. and Sutcliffe, M. (1988) Eur.J Biochem. 173, 513-520.
12. Topham, C.M., McLeod, A., Eisenmenger, F., Overington, J.P., Johnson, M.S. and Blundell, T.L. (1993) J.Mol.Biol. 229, 194-220.
13. Donate, L.E., Rufino, S., Canard, L. and Blundell, T.L. (1996) Prot.Sci. 5, 2600-2616.
14. Rufino, S., Donate, L.E. and Blundell, T.L. (1997) J.Mol.Biol. 267. In press.
15. Emtage, J.S., Angal, S., Doel, M.T., Harris, T.J.R., Jenkins, B., Lilley, G. and Lowe, P.A. (1983). Proc.Nat.Acad.Sci., 80, 3671-3675.
16. Pitts, J.E., Dhanaraj, V., Dealwis, C., Mantafounis, D., Nugent, P.G., Orprayoon, P., Cooper, J.B. Newman, M. and Blundell, T.L. (1992) Scand.J.Clin.Lab.Invest. 52, 39-50.
17. Pitts, J.E., Uusitalo, J.M., Mantafounis, D., Nugent, P.G., Quinn, D.D., Orprayoon, P. and Pentilla, M. (1993) J.Biotechnology. 28, 69-83.
18. Harkki, A., Uusitalo, J., Bailey, M., Penttilla, M. and Knowles, J.K.C. (1989) Bio-Technology, 7, 596-602.
19. Nugent, P.G., Albert, A., Orprayoon, P., Wilsher, J., Pitts, J.E., Blundell, T.L. and Dhanaraj, V. (1996). Prot.Engg. 9, 885-893.