Group 13 allergens as environmental and immunological markers for grass pollen allergy: Studies by immunogold field emission scanning and transmission electron microscopy

International Archives of Allergy and Immunology

Volumn 136, Issue 4, 2005, Pages 303-310

  Grote, Monika ^a   Swoboda, Ines ^b   Valenta, Rudolf ^b   Reichelt, Rudolf ^a  

^a UNIVERSITY OF MÜNSTER   (Germany)

^b UNIVERSITY OF VIENNA   (Austria)

Author keywords

Allergen; Grass pollen; Immunoblotting; Immunogold electron microscopy; Phl p 13; Poaceae; Release kinetics

Indexed keywords

ALLERGEN; BIOLOGICAL MARKER; IMMUNOGLOBULIN E ANTIBODY; POLYSACCHARIDE; PYROXYLIN; RABBIT ANTISERUM; RAIN; PHL P 13 PROTEIN, PHLEUM PRATENSE; VEGETABLE PROTEIN;

ANALYTIC METHOD; ARTICLE; CELL RESPIRATION; CELL WALL; CYTOPLASM; GRASS; GRASS POLLEN; HYDRATION; IMMUNOBLOTTING; IMMUNOGOLD ELECTRON MICROSCOPY; KINETICS; MICROTUBULE; MOLECULAR PROBE; NONHUMAN; PLANT CELL; POACEAE; POLLEN ALLERGY; PRIORITY JOURNAL; PURIFICATION; SENSITIZATION; SUPERNATANT; TRANSMISSION ELECTRON MICROSCOPY; ULTRASTRUCTURE ANALYSIS AND ELECTRON MICROSCOPY; CLASSIFICATION; ELECTRON MICROSCOPY; IMMUNOHISTOCHEMISTRY; IMMUNOLOGY; OSMOTIC PRESSURE; POLLEN; ULTRASTRUCTURE;

ALLERGENS; IMMUNOHISTOCHEMISTRY; MICROSCOPY, ELECTRON; OSMOTIC PRESSURE; PLANT PROTEINS; POACEAE; POLLEN;

EID: 18244397732     PISSN: 10182438     EISSN: None     Source Type: Journal    
DOI: 10.1159/000083975     Document Type: Article

References (22)

1. Lockey RF, Bukantz SC: Allergens and Allergen Immunotherapy. New York, Dekker, 1998.
2. Grote M: In situ localization of pollen allergens by immunogold electron microscopy: Allergens at unexpected sites. Int Arch Allergy Immunol 1999;118:1-6.
3. Grote M, Vrtala S, Valenta R: Monitoring of two allergens, Bet v I and profilin, in dry and rehydrated birch pollen by immunogold electron microscopy and immunoblotting. J Histochem Cytochem 1993;41:745-750.
4. Vrtala S, Grote M, Duchêne M, van Ree R, Kraft D, Scheiner O, Valenta R: Properties of tree and grass pollen allergens: Reinvestigation of the linkage between solubility and allergenicity. Int Arch Allergy Immunol 1993;102:160-169.
5. Suphioglu C, Singh MB, Taylor P, Bellomo R, Holmes P, Puy R, Knox RB: Mechanism of grass-pollen-induced asthma. Lancet 1992;339:569-572.
6. Grote M, Vrtala S, Niederberger V, Valenta R, Reichelt R: Expulsion of allergen-containing materials from hydrated rye grass (Lolium perenne) pollen revealed by immungold field emission scanning and transmission electron microscopy. J Allergy Clin Immunol 2000;105:1140-1145.
7. Grote M, Vrtala S, Niederberge V, Wiermann R, Valenta R, Reichelt R: Release of allergen-bearing cytoplasm from hydrated pollen: A mechanism common to a variety of grass (Poaceae) species revealed by electron microscopy. J Allergy Clin Immunol 2001;108:109-115.
8. Grote M, Valenta R, Reichelt R: Abortive pollen germination: A mechanism of allergen release in birch, alder, and hazel revealed by immunogold electron microscopy. J Allergy Clin Immunol 2003;111:1017-1023.
9. Freidhoff LR, Ehrlich-Kautzky E, Grant JH, Meyers DA, Marsh DG: A study of the human immune response to Lolium perenne (rye) pollen and its components, Lol p I and Lol p II (rye I and rye II). J Allergy Clin Immunol 1986;78:190-201.
10. Andersson K, Lidholm J: Characteristics and immunobiology of grass pollen allergens. Int Arch Allergy Immunol 2003;130:87-107.
11. Johnson P, Marsh DG: 'Isoallergens' from rye grass pollen. Nature 1965;206:935.
12. Suck R, Petersen A, Hagen S, Cromwell O, Becker WM, Fiebig H: Complementary DNA cloning and expression of a novel high molecular weight allergen Phl p 13 from timothy grass (Phleum pratense). Clin Exp Allergy 2000;30:324-332.
13. Petersen A, Suck R, Hagen S, Cromwell O, Fiebig H, Becker WM: Group 13 grass pollen allergens: Structural variability between different grass species and analysis of proteolytic stability. J Allergy Clin Immunol 2001;107:856-862.
14. Swoboda I, Grote M, Verdino P, Keller W, Singh MB, De Weerd N, Sperr WR, Valent P, Reichelt R, Fiebig F, Valenta R, Spitzauer S: Molecular characterization of polygalacturonases as grass pollen-specific marker allergens: Expulsion from hydrated pollen via submicronic particles. J Immunol 2004;172:6490-6500.
15. Laemmli UK: Cleavage of structural proteins during the assembly of the head of the bacteriophage T4. Nature 1970;227:680-685.
16. Bradford MM: A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye-binding. Anal Biochem 1976;72:248-254.
17. Valenta R, Duchêne M, Ebner C, Valent P, Sillaber C, Deviller P, Ferreira F, Tejkl M, Edelmann H, Kraft D, Scheiner O: Profilins constitute a novel family of functional plant pan-allergens. J Exp Med 1992;175:377-385.
18. Grote M, Dolecek C, van Ree R, Valenta R: Immunogold electron microscopic localization of timothy grass (Phleum pratense) pollen major allergens, Phl p I and Phl p V, after anhydrous fixation in acrolein vapor. J Histochem Cytochem 1994;42:427-431.
19. Heslop-Harrison J, Heslop-Harrison Y: The growth of the grass pollen tube. 1. Characteristics of the polysaccharide particles (P-particles) associated with apical growth. Protoplasma 1982;112:71-80.
20. Heslop-Harrison J, Heslop-Harrison Y: The pollen-stigma interaction in the grasses. 1. Fine-structure and cytochemistry of the stigmas of Hordeum and Secale. Acta Bot Neerl 1980;29:261-276.
21. Heslop-Harrison J, Heslop-Harrison Y: The pollen-stigma interactions in the grasses. 2. Pollen tube penetration and the stigma response in Secale. Acta Bot Neerl 1981;30:289-307.
22. Suphioglu C: Thunderstorm asthma due to grass pollen. Int Arch Allergy Immunol 1998;116:253-260.