Purification and characterization of the 15-kDa protein from the sperm flagella of salmonid fishes

Biomedical Research

Volumn 24, Issue 4, 2003, Pages 153-164

  Itoh, Atsuko ^a,c   Fujinoki, Masakatsu ^b   Kawamura, Takeshi ^c   Inaba, Kazuo ^d   Ohtake, Hideki ^b   Shimizu, Nobuyoshi ^c   Morisawa, Masaaki ^a  

^a UNIVERSITY OF TOKYO   (Japan)

^b DOKKYO MEDICAL UNIVERSITY   (Japan)

^c KEIO UNIVERSITY SCHOOL OF MEDICINE   (Japan)

^d TOHOKU UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

FISH PROTEIN; PHOSPHOTYROSINE; POLYCLONAL ANTIBODY; TUBULIN;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ANIMAL EXPERIMENT; ARTICLE; FLAGELLUM; IMMUNOBLOTTING; JAPAN; MALE; MASS SPECTROMETRY; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN ANALYSIS; PROTEIN DETERMINATION; PROTEIN FUNCTION; PROTEIN PURIFICATION; PROTEIN STRUCTURE; RAINBOW TROUT; REVERSED PHASE LIQUID CHROMATOGRAPHY; SALMONID; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY; SPERMATOGONIUM; SPERMATOZOON MOTILITY; SUCROSE DENSITY GRADIENT CENTRIFUGATION;

ANIMALIA; ONCORHYNCHUS MYKISS; PISCES; SALMONIDAE;

EID: 18244365431     PISSN: 03886107     EISSN: 1880313X     Source Type: Journal    
DOI: 10.2220/biomedres.24.153     Document Type: Article

References (36)

1. Brokaw, C. J. (1987) Regulation of sperm flagellar motility by calcium and cAMP-dependent phosphorylation. J. Cell Biochem. 33, 175-184.
2. Dey, C. S. and Brokaw, C. J. (1991) Activation of Ciona sperm motility: phosphorylation of dynein polypeptides and effects of a tyrosine kinase inhibitor. J. Cell Sci. 100, 815-824.
3. Fujinoki, M., Ohtake, H. and Okuno, M. (2001) Serine phosphorylation of flagellar proteins associated with motility activation of hamster spermatozoa. Biomed. Res. 22, 45-58.
4. Fujinoki, M., Ohtake, H. and Okuno, M. (2001) Tyrosine phosphorylation and dephosphorylation associated with motility of hamster spermatozoa. Biomed. Res. 22, 147-155.
5. Fujinoki, M., Kawamura T., Toda, T., Ohtake H., Ishimoda-Takagi, T., Shimizu, N., Yamaoka, S. and Okuno, M. (2003) Identification of 36k-Da flagellar phosphoproteins associated with hamster sperm motility. J. Biochem. 133, 361-369.
6. Goldstein, S. F. (1979) Strong transients in sea urchin sperm flagella. J. Cell Biol. 80, 61-68.
7. Hayashi, H., Yamamoto, K., Yonekawa, H. and Morisawa, M. (1987) Involvement of tyrosine protein kinase in the initiation of flagellar movement in rainbow trout spermatozoa. J. Biol. Chem. 262, 16692-16698.
8. Horowitz, J. A., Wasco, W., Leiser, M. and Orr, G. A. (1988) Interaction of the regulatory subunit of a type II cAMP-dependent protein kinase with mammalian sperm flagellum. J. Biol. Chem. 263, 2098-2104.
9. Inaba, K., Morisawa, S. and Morisawa, M. (1998) Proteasomes regulate the motility of salmonid fish sperm through modulation of cAMP-dependent phosphorylation of an outer arm dynein light chain. J. Cell Sci. 111, 1105-11115.
10. Inaba, K., Kagami, O. and Ogawa, K. (1999) Tctex2-related outer arm dynein light chain is phosphorylated at activation of sperm motility. Biochem. Biophys. Res. Commun. 256, 177-183.
11. Inaba, K. (2002) Dephosphorylation of Tctex2-related dynein light chain by type 2A protein phosphatase. Biochem. Biophys. Res. Commun. 297, 800-805.
12. Ishiguro, K., Murofushi, H. and Sakai, H. (1982) Evidence that cAMP-dependent protein kinase and protein factor are involved in reactivation of Triton X-100 models of sea urchin and starfish spermatozoa. J. Cell Biol. 92, 777-782.
13. Itoh, A., Inaba, K., Fujinoki, M. and Morisawa, M. (2001) Motility-associated and cyclic AMP-dependent protein phosphorylation in the sperm of the chum salmon, Oncorhynchus keta. Biomed. Res. 22, 241-248.
14. Jin, Z. X., Nakajima, T., Morisawa, M. and Hayashi, H. (1994). Isolation and properties of a protein complex containing flagellar movement-initiating phosphoprotein from testes of a white salmon. J. Biochem. 115, 552-556.
15. Jin, Z. X., Inaba, K., Morisawa, M. and Hayashi, H. (1994) Monoclonal antibodies against the protein complex that contains the flagellar movement-initiating phosphoprotein of Oncorhynchus keta. J. Biochem. 115, 885-890.
16. Kho, K. H., Tanimoto, S., Inaba, K., Oka, Y. and Morisawa, M. (2001) Transmembrane cell signaling for the initiation of trout sperm motility: Roles of ion channels and membrane hyperpolarization for cyclic AMP synthesis. Zool. Sci. 18, 919-928.
17. Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
18. Morisawa, M. and Okuno, M. (1982) Cyclic AMP induces maturation of trout sperm axoneme to initiate motility. Nature 41, 703-704.
19. Morisawa, M. and Hayashi, H. (1985) Phosphorylation of a 15K axonemal protein is the trigger initiating trout sperm motility. Biomed. Res. 6, 181-184.
20. Morisawa, M. (1994) Cell signaling mechanisms for sperm motility. Zool. Sci., 11, 647-662.
21. Nishi, N. (1994) Protein solubilization. In Theory and Practice on Enzymes and Other Proteins (T. Horio, ed), pp. 62-64 Nankou-dou, Tokyo, Japan. [Japanese]
22. Nomura, M., Inaba, K. and Morisawa, M. (2000) Cyclic AMP- and calmodulin-dependent phosphorylation of 21 and 26 kDa proteins in axoneme is a prerequisite for SAAF-induced motile activation in ascidian spermatozoa. Dev. Growth Differ. 42, 129-138.
23. Okuno, M. and Hiramoto, Y. (1976) Mechanical stimulation of starfish sperm flagella. J. Exp. Biol. 65, 401-413.
24. Okuno, M. (1982) Mechanism of flagellar movement in spermatozoa. In Spermatology (H. Mohri, supervising ed., M. Morisawa, and M. Hoshi, eds.), pp. 80-103 the University of Tokyo press, Tokyo, Japan. [Japanese]
25. Okuno, M. and Morisawa, M. (1989) Effects of calcium on motility of rainbow trout sperm flagella demembranated with Triton X-100. Cell Motil. Cytoskel. 14, 194-200.
26. Opresko, L. K. and Brokaw, C. J. (1983) cAMP-dependent phosphorylation associated with activation of motility of Ciona sperm flagella. Gamete. Res. 8, 201-218.
27. Schagger, H. and Jagow, G. V. (1987) Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal. Biochem. 166, 368-379.
28. Si, Y. and Okuno, M. (1995) Activation of mammalian sperm motility by regulation of microtubule sliding via cyclic adenosine 5′-monophosphate-dependent phosphorylation. Biol. Reprod. 53, 1081-1087.
29. Si, Y. and Okuno, M. (1999) Regulation of microtubule sliding by a 36-kDa phosphoprotein in hamster sperm flagella. Mol. Biol. Dev. 52, 328-334.
30. Simpson, R. J., Moritz, R. L., Nice, E. E. and Gergo, B. (1987) A high-performance liquid chromatography procedure for recovering subnanomole amounts of protein from SDS-gel electroeluates for gas-phase sequence analysis. Eur. J. Biochem. 165, 21-29.
31. Stephens, R. E. and Prior, G. (1992) Dynein from serotonin-activated cilia and flagella: extraction characteristics and distinct sites for cAMP-dependent protein phosphorylation. J. Cell Sci. 103, 999-1012.
32. Tash, J. S., Kakar, S. S. and Means, A. R. (1984) Flagellar motility requires the cAMP-dependent phosphorylation of heat-stable NP-40-soluble 56 kd protein, Axokinin. Cell 38, 551-559.
33. Tash, J. S., Hidaka, H. and Means, A. R. (1986) Axokinin phosphorylation by cAMP-dependent protein kinase is sufficient for activation of sperm flagellar motility. J. Cell Biol. 103, 649-645.
34. Tash, J. S., Krinks, M., Patel, J., Means, R. L., Klee, C. B. and Means, A. R. (1988) Identification, characterization, and functional correlation of calmodulin-dependent protein phosphatase in sperm. J. Cell Biol. 106, 1625-1633.
35. Towbin, H., Staehelin, T. and Gordon, J. (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc. Natl. Acad. Sci. USA 76, 4350-4354.
36. Vesterberg, O. (1971) Isoelectric focusing of proteins. In Methods in Enzymology Vol. 22 (W. B. Jakoby, ed.) pp. 394-412 Academic Press, New York.