Efficient generation of human T cells from a tissue-engineered thymic organoid

Nature Biotechnology

Volumn 18, Issue 7, 2000, Pages 729-734

  Poznansky, Mark C ^a   Evans, Richard H ^a   Foxall, Russell B ^a   Olszak, Ivona T ^a   Piascik, Anita H ^a   Hartman, Kelly E ^a   Brander, Christian ^a   Meyer, Thomas H ^b   Pykett, Mark J ^b   Chabner, Karissa T ^a   Kalams, Spyros A ^a   Rosenzweig, Michael ^b   Scadden, David T ^a  

^a MASSACHUSETTS GENERAL HOSPITAL   (United States)

^b Cytomatrix   (United States)

Author keywords

Hematopoiesis; Stem cells; T lymphocytes; Thymus

Indexed keywords

CD3 ANTIGEN; CD34 ANTIGEN; T LYMPHOCYTE RECEPTOR;

ANIMAL TISSUE; ARTICLE; BIOCOMPATIBILITY; BONE REMODELING; COCULTURE; CONTROLLED STUDY; GENETIC ENGINEERING; HEMATOPOIETIC STEM CELL; HUMAN; HUMAN CELL; LYMPHOCYTE DIFFERENTIATION; LYMPHOPOIESIS; MOUSE; NONHUMAN; PRIORITY JOURNAL; T LYMPHOCYTE SUBPOPULATION; THYMUS;

ANIMALS; ANTIGENS, CD; ANTIGENS, CD34; ARTIFICIAL ORGANS; BONE MARROW CELLS; CARBON; COATED MATERIALS, BIOCOMPATIBLE; COCULTURE TECHNIQUES; CULTURE TECHNIQUES; FLOW CYTOMETRY; GLYCOPROTEINS; HEMATOPOIETIC STEM CELLS; HIV-1; HUMANS; IMMUNOPHENOTYPING; MICE; MICE, INBRED C57BL; MICROSCOPY, ELECTRON, SCANNING; ORGANOIDS; PEPTIDES; POLYMERASE CHAIN REACTION; T-LYMPHOCYTES; THYMUS GLAND; TIME FACTORS;

ANIMALIA; MURINAE;

EID: 18144448857     PISSN: 10870156     EISSN: None     Source Type: Journal    
DOI: 10.1038/77288     Document Type: Article

References (27)

1. Bobyn, J.D., Stackpool, G.J., Hacking, S.A., Tanzer, M. & Krygier, J.J. Characteristics of bone ingrowth and interface mechanics of a new porous tantalum biomaterial. J. Bone Joint Surg. Br. 81, 907-914 (1999).
2. Black, J. Biological performance of tantalum. Clin. Mater. 16, 167-173 (1994).
3. Boyd, R.L. et al. The thymic microenvironment. Immunol. Today 14, 445-459 (1993).
4. Van Vliet, E., Melis, M. & Van Ewijk, W. Monoclonal antibodies to stromal cell types of the mouse thymus. Eur. J. Immunol. 14, 524-529 (1984).
5. Van Vilet, E., Jenkinson, E.J., Kingston, R., Owen, J.J. & Van Ewijk, W. Stromal cell types in the developing thymus of the normal and nude mouse embryo. Eur. J. Immunol. 15, 675-681 (1985).
6. van Ewijk, W. Cell surface topography of thymic microenvironments. Lab. Invest. 59, 579-590 (1988).
7. van Ewijk, W. T-cell differentiation is influenced by thymic microenvironments. Annu. Rev. Immunol. 9, 591-615 (1991).
8. Freedman, A.R., Zhu, H., Levine, J.D., Kalams, S. & Scadden, D.T. Generation of human T lymphocytes from bone marrow CD34+ cells in vitro. Nat. Med. 2, 46-51 (1996).
9. Gardner, J.P. et al. T-lymphopoietic capacity of cord blood-derived CD34+ progenitor cells. Exp. Hematol. 26, 991-999 (1998).
10. Pawelec, G., Muller, R., Rehbein, A., Hahnel, K. & Ziegler, B.L. Extrathymic T cell differentiation in vitro from human CD34+ stem cells. J. Leukoc. Biol. 64, 733-739 (1998).
11. Galy, A., Verma, S., Barcena, A. & Spits, H. Precursors of CD3+CD4+CD8+ cells in the human thymus are defined by expression of CD34. Delineation of early events in human thymic development. J. Exp. Med. 178, 391-401 (1993).
12. Anderson, G., Jenkinson, E.J., Moore, N.C. & Owen, J.J. MHC class II-positive epithelium and mesenchyme cells are both required for T-cell development in the thymus. Nature 362, 70-73 (1993).
13. Barcena, A. et al. Phenotypic and functional analysis of T-cell precursors in the human fetal liver and thymus: CD7 expression in the early stages of T- and myeloid-cell development. Blood 82, 3401-3414 (1993).
14. Barcena, A. et al. Lymphoid and myeloid differentiation of fetal liver CD34+ lineage- cells in human thymic organ culture. J. Exp. Med. 180, 123-132 (1994).
15. Fisher, A.G. et al. Human thymocyte development in mouse organ cultures. Int. Immunol. 2, 571-578 (1990).
16. Yeoman, H. et al. Human bone marrow and umbilical cord blood cells generate CD4+ and CD8+ single-positive T cells in murine fetal thymus organ culture. Proc. Natl. Acad. Sci. USA 90, 10778-10782 (1993).
17. Res, P. et al. CD34+CD38dim cells in the human thymus can differentiate into T, natural killer, and dendritic cells but are distinct from pluripotent stem cells. Blood 87, 5196-5206 (1996).
18. Plum, J., De Smedt, M., Defresne, M.P., Leclercq, G. & Vandekerckhove, B. Human CD34+ fetal liver stem cells differentiate to T cells in a mouse thymic microenvironment. Blood 84, 1587-1593 (1994).
19. Shortman, K., Egerton, M., Spangrude, G.J. & Scollay, R. The generation and fate of thymocytes. Semin. Immunol. 2, 3-12 (1990).
20. van Ewijk, W. et al. Thymic microenvironments, 3-D versus 2-D? Semin. Immunol. 11, 57-64 (1999).
21. Fujimoto, S. & Yamagishi, H. Isolation of an excision product of T-cell receptor alpha-chain gene rearrangements . Nature 327, 242-243 (1987).
22. Okazaki, K., Davis, D.D. & Sakano, H. T cell receptor beta gene sequences in the circular DNA of thymocyte nuclei: direct evidence for intramolecular DNA deletion in V-D-J joining. Cell 49, 477-485 (1987).
23. Livak, F. & Schatz, D.G. T-cell receptor alpha locus V(D)J recombination by-products are abundant in thymocytes and mature T cells. Mol. Cell Biol. 16, 609-618 (1996).
24. Takeshita, S., Toda, M. & Yamagishi, H. Excision products of the T cell receptor gene support a progressive rearrangement model of the alpha/delta locus. EMBO J. 8, 3261-3270 (1989).
25. Douek, D.C. et al. Changes in thymic function with age and during the treatment of HIV infection. Nature 396, 690-695 (1998).
26. Genevee, C. et al. An experimentally validated panel of subfamily-specific oligonucleotide primers (V alpha 1-w29/V beta 1-w24) for the study of human T cell receptor variable V gene segment usage by polymerase chain reaction. Eur. J. Immunol. 22, 1261-1269 (1992).
27. Zhang, X.M., Cathala, G., Soua, Z., Lefranc, M.P. & Huck, S. The human T-cell receptor gamma variable pseudogene V10 is a distinctive marker of human speciation. Immunogenetics 43, 196-203 (1996).