Red fluorescent protein eqFP611 and its genetically engineered dimeric variants

Journal of Biomedical Optics

Volumn 10, Issue 1, 2005, Pages

  Wiedenmann, Jörg ^a   Vallone, Beatrice ^b   Renzi, Fabiana ^b   Nienhaus, Karin ^a   Ivanchenko, Sergey ^a   Röcker, Carlheinz ^a   Nienhaus, G Ulrich ^a,c  

^a UNIVERSITY OF ULM   (Germany)

^b SAPIENZA UNIVERSITY OF ROME   (Italy)

^c University of Illinois at Urbana Champaign   (United States)

Author keywords

Crystal structure; Dimer; DsRed; EqFP611; Green fluorescent protein; Monomer; Red fluorescent protein; Single molecule

Indexed keywords

AMINO ACIDS; CRYSTAL STRUCTURE; DIMERS; DISSOCIATION; FLUORESCENCE; GENETIC ENGINEERING; INTERFACES (MATERIALS); MOLECULAR WEIGHT; MONOMERS;

AEQUOREA VICTORIA; EQFP611; GREEN FLUORESCENT PROTEIN; RED FLUORESCENT PROTEIN; SINGLE MOLECULE;

PROTEINS;

ACTINIARIA; ANEMONE; ANTHOZOA; ENTACMAEA QUADRICOLOR;

EID: 18144379506     PISSN: 10833668     EISSN: 15602281     Source Type: Journal    
DOI: 10.1117/1.1854680     Document Type: Article

References (33)

1. D. C. Prasher, V. K. Eckenrode, W. W. Ward, F. G. Prendergast, and M. J. Cormier, "Primary structure of the Aequorea victoria greenfluorescent protein," Gene 111, 229-233 (1992).
2. M. Chalfie, Y. Tu, G. Euskirchen, W. W. Ward, and D. C. Prasher, "Green fluorescent protein as a marker for gene expression," Science 263, 802-805 (1994). (Pubitemid 24093206)
3. R. Y. Tsien, "The green fluorescent protein," Annu. Rev. Biochem. 67, 509-544 (1998). (Pubitemid 28411137)
4. J. Wiedenmann, "Die Anwendung eines orange fluoreszierenden Proteins und weiterer farbiger Proteine und der zugehörenden gene aus der Artengruppe Anemonia sp. (sulcata) Pennant, (Cnidaria, Anthozoa, Actinaria) in Gentechnologie und Molekularbiologie," Offenlegungsschrift DE 197 18 640 A1, Deutsches Patent-und Markenamt, pp. 1-18 (1997).
5. J. Wiedenmann, C. Elke, K. D. Spindler, and W. Funke, "Cracks in the b-can: Fluorescent proteins from Anemonia sulcata (Anthozoa, Actinaria)," Proc. Natl. Acad. Sci. U.S.A. 97, 14091-14096 (2000). (Pubitemid 32016535)
6. M. V. Matz, A. F. Fradkov, Y. A. Labas, A. P. Savitsky, A. G. Zaraisky, M. L. Markelov, and S. A. Lukyanov, "Fluorescent proteins from nonbioluminescent Anthozoa species," Nat. Biotechnol. 17, 969-973 (1999). (Pubitemid 29474851)
7. A. F. Fradkov, Y. Chen, L. Ding, E. V. Barsova, M. V. Matz, and S. A. Lukyanov, "Novel fluorescent protein from Discosoma coral and its mutants possesses a unique far-red fluorescence," FEBS Lett. 479, 127-130 (2000). (Pubitemid 30616419)
8. J.Wiedenmann, C. Röcker, andW. Funke, "The morphs of Anemonia aff. sulcata (cnidaria, anthozoa) in particular consideration of the ectodermal pigments," in Verhandlungen der Gesellschaft für Okologie Band 29, J. Pfadenhauer, Ed., pp. 497-503, Spektrum Akademischer Verlag, Berlin (1999). (Pubitemid 30037456)
9. A. Salih, A. Larkum, G. Cox, M. Kühl, and O. Hoegh-Guldberg, "Fluorescent pigments in corals are photoprotective," Nature (London) 408, 850-853 (2000). (Pubitemid 32016093)
10. S. G. Dove, O. Hoegh-Guldberg, and S. Ranganathan, "Major colour patterns of reef-building are due to a family of GFP-like proteins," Coral Reefs 19, 197-204 (2001). (Pubitemid 32143287)
11. J. Wiedenmann, S. Ivanchenko, F. Oswald, and G. U. Nienhaus, "Identification of GFP-like proteins in non-bioluminescent, azooxanthellate Anthozoa opens new perspectives for bioprospecting," Mar. Biotechnol. 6, 6270-6277 (2004).
12. G. S. Baird, D. A. Zacharias, and R. Y. Tsien, "Biochemistry, mutagenesis, and oligomerization of DsRed, a red fluorescent protein from coral," Proc. Natl. Acad. Sci. U.S.A. 97, 11984-11989 (2000).
13. R. E. Campbell, O. Tour, A. E. Palmer, P. A. Steinbach, G. S. Baird, D. A. Zacharias, and R. Y. Tsien, "A monomeric red fluorescent protein," Proc. Natl. Acad. Sci. U.S.A. 99, 7877-7882 (2002). (Pubitemid 34650973)
14. J. Wiedenmann, A. Schenk, C. Röcker, K. D. Spindler, and G. U. Nienhaus, "A far-red fluorescent protein with fast maturation and reduced oligomerization tendency from Entacmaea quadricolor (Cnidaria, Anthozoa, Actinaria)," Proc. Natl. Acad. Sci. U.S.A. 99, 11646-11651 (2002). (Pubitemid 34994453)
15. H. v. Gill, "Calculation of protein extinction coefficients from amino acid sequence data," Anal. Biochem. 182, 319-326 (1989).
16. J. Olmsted, "Calorimetric determinations of absolute fluorescence quantum yields," J. Phys. Chem. 83(20), 2581-2584 (1979).
17. K. Nienhaus, B. Vallone, F. Renzi, J. Wiedenmann, and G. U. Nienhaus, "Crystallization and preliminary x-ray diffraction analysis of the red fluorescent protein eqFP611," Acta Cryst. D59, 1253-1255 (2003). (Pubitemid 36872369)
18. M. A. Wall, M. Socolich, and R. Ranganathan, "The structural basis for red fluorescence in the tetrameric GFP homolog DsRed," Nat. Struct. Biol. 12, 1133-1138 (2000).
19. Collaborative Computational Project Nr 4, The CCP4 suite: programs for protein crystallographyy, Acta Cryst. D50, 760-763 (1994).
20. J. Petersen, P. G. Wilmann, T. Beddoe, A. J. Oakley, R. J. Devenish, M. Prescott, and J. Rossjohn, "The 2.0-A crystal structure of eqFP611, a far red fluorescent protein from the sea anemone Entacmaea quadricolor," J. Biol. Chem. 278, 44626-44631 (2003). (Pubitemid 37377217)
21. B. Lounis, J. Deich, F. I. Rosell, S. G. Boxer, and W. E. Moerner, "Photophysics of DsRed, a red fluorescent protein, from the ensemble to the single-molecule level," J. Phys. Chem. B 105, 5048-5054 (2001). (Pubitemid 35372670)
22. A. Schenk, S. Ivanchenko, C. Röcker, J. Wiedenmann, and G. U. Nienhaus, "Photodynamics of Red Fluorescent Proteins Studied by Fluorescence Correlation Spectroscopy," Biophys. J. 86, 384-394 (2004). (Pubitemid 38067466)
23. D. Yarbrough, R. M. Wachter, K. Kallio, M. V. Matz, and S. J. Remington, "Refined crystal structure of DsRed, a red fluorescent protein from coral, at 2.0-A resolution," Proc. Natl. Acad. Sci. U.S.A. 98, 462-467 (2001). (Pubitemid 32105064)
24. M. Prescott, M. Ling, T. Beddoe, A. J. Oakley, S. Dove, O. Hoegh-Guldberg, R. J. Devenish, and J. Rossjohn, "The 2.2 A crystal structure of a pocilloporin pigment reveals a nonplanar chromophore conformation," Structure (Camb) 11, 275-284 (2003). (Pubitemid 36287692)
25. V. V. Verkhusha and K. A. Lukyanov, "The molecular properties and applications of Anthozoa fluorescent proteins and chromoproteins," Nat. Biotechnol. 22, 289-296 (2004). (Pubitemid 38295761)
26. Y. G. Yanushevich, D. B. Staroverov, A. P. Savitsky, A. F. Fradkov, N. G. Gurskaya, M. E. Bulina, K. A. Lukyanov, and S. A. Lukyanov, "A strategy for the generation of non-aggregating mutants of Anthozoa fluorescent proteins," FEBS Lett. 511, 11-14 (2002). (Pubitemid 34127814)
27. N. G. Gurskaya, A. F. Fradkov, A. Terskikh, M. V. Matz, Y. A. Labas, V. I. Martynov, Y. G. Yanushevich, K. A. Lukyanov, and S. A. Lukyanov, "GFP-like chromoproteins as a source of far-red fluorescent proteins," FEBS Lett. 507, 16-20 (2001). (Pubitemid 33000290)
28. G. S. Baird, PhD Thesis, Univ. California, San Diego (2001).
29. D. G. Fautin, "Hexacorallians of the world. Sea anemones, corals and their allies," see http://hercules.kgs.ku.edu/hexacoral/anemone2/index. cfm.
30. D. A. Zacharias, J. D. Violin, A. C. Newton, and R. Y. Tsien, "Partitioning of lipid-modified monomeric GFPs into membrane microdomains of live cells," Science 296, 913-916 (2002). (Pubitemid 34464897)
31. D. A. Shagin, E. V. Barsova, Y. G. Yanushevich, A. F. Fradkov, K. A. Lukyanov, Y. A. Labas, T. N. Semenova, J. A. Ugalde, A. Meyers, J. M. Nunez, E. A. Widder, S. A. Lukyanov, and M. V. Matz, "GFP-like proteins as ubiquitous metazoan superfamily: evolution of functional features and structural complexity," Mol. Biol. Evol. 21, 841-850 (2004). (Pubitemid 38529726)
32. P. Wilkens, "Ordnung Corallimorpharia Korallenanemonen," in Niedere Tiere-Steinkorallen, Scheiben-und Krustenanemonen, pp. 67-100, Engelbert Pfriem Verlag, Wuppertal (1987).
33. S. Sprung and J. C. Delbeek, "The identification and care of Corallimorpharians," in The Reef Aquarium, Vol. 2, pp. 313-351, Ricordea Publishing, Coconut Grove, FL (1997).