Housekeeping gene expression during fetal brain development in the rat - Validation by semi-quantitative RT-PCR

Developmental Brain Research

Volumn 156, Issue 1, 2005, Pages 38-45

  Al Bader, Maie Dawoud ^a   Al Sarraf, Hameed Ali ^a  

^a KUWAIT UNIVERSITY   (Kuwait)

Author keywords

18S rRNA; Cyclophilin; Glyceraldehydes 3 phosphate dehydrogenase; Histone H4; Housekeeping genes; Ribosomal protein L19; Glucuronidase

Indexed keywords

BETA GLUCURONIDASE; CYCLOPHILIN; GENE PRODUCT; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; HISTONE H4; MESSENGER RNA; RIBOSOMAL PROTEIN L19; RIBOSOME PROTEIN; RNA 18S; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BRAIN DEVELOPMENT; CONTROLLED STUDY; FEMALE; FETUS; FETUS DEVELOPMENT; GENE EXPRESSION; GENE IDENTIFICATION; GENETIC ANALYSIS; GESTATION PERIOD; HOUSEKEEPING GENE; MALE; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; RAT; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; VALIDATION PROCESS;

MAMMALIA;

EID: 18044374087     PISSN: 01653806     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.devbrainres.2005.01.010     Document Type: Article

References (23)

1. J.L. Aerts, M.I. Gonzales, S.L. Topalian, Selection of appropriate control genes to assess expression of tumor antigens using real-time RT-PCR. Biotechniques. 36 (2004) 84-86, 88, 90-91.
2. R. Aloni, D. Peleg, and O. Meyuhas Selective translational control and nonspecific posttranscriptional regulation of ribosomal protein gene expression during development and regeneration of rat liver Mol. Cell. Biol. 12 1992 2203 2212
3. M.C. Botte, A.M. Chamagne, M.C. Carre, R. Counis, and M.L. Kottler Fetal expression of GnRH and GnRH receptor genes in rat testis and ovary J. Endocrinol. 159 1998 179 189
4. E.L. Calvo, C. Boucher, Z. Coulombe, and J. Morisset Pancreatic GAPDH expression during ontogeny and acute pancreatitis induced by caerulein Biochem. Biophys. Res. Commun. 235 1997 636 640
5. Y.L. Chan, A. Lin, J. McNally, D. Peleg, O. Meyuhas, and I.G. Wool The primary structure of rat ribosomal protein L19. A determination from the sequence of nucleotides in a cDNA and from the sequence of amino acids in the protein J. Biol. Chem. 262 1987 1111 1115
6. P. Chomczynski, and N. Sacchi Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction Anal. Biochem. 162 1987 156 159
7. P. Fort, L. Marty, M. Piechaczyk, S. el Sabrouty, C. Dani, P. Jeanteur, and J.M. Blanchard Various rat adult tissues express only one major mRNA species from the glyceraldehyde-3-phosphate-dehydrogenase multigenic family Nucleic Acids Res. 13 1985 1431 1442
8. S. Grimes, P. Weisz-Carrington, H. Daum III, J. Smith, L. Green, K. Wright, G. Stein, and J. Stein A rat histone H4 gene closely associated with the testis-specific H1t gene Exp. Cell Res. 173 1987 534 545
9. M.W. Harding, R.E. Handschumacher, and D.W. Speicher Isolation and amino acid sequence of cyclophilin J. Biol. Chem. 261 1986 8547 8555
10. M. Jakubowski, M. Blum, and J. Roberts Postnatal development of gonadotropin-releasing hormone and cyclophilin gene expression in the female and male rat brain Endocrinology 128 1991 2702 2708
11. R.D. Kornberg Structure of chromatin Annu. Rev. Biochem. 46 1977 931 954
12. R. Kumar, and R.W. Joyner Expression of protein phosphatases during postnatal development of rabbit heart Mol. Cell. Biochem. 245 2003 91 98
13. V.A. McKusick, and G.F. Neufeld J.B. Wyngaarden D.D. Fredrickson J.L. Goldstein M.S. Brown The metabolic basis of inherited disease 1983 McGraw-Hill New York 751 777
14. R.K. Murray Biochemical properties of cancer cells I.F. Tannock R.P. Hill The basic science of oncology 1987 Pergamon Press New York 176 191
15. C. Robert, S. McGraw, L. Massicotte, M. Pravetoni, F. Gandolfi, and M.A. Sirard Quantification of housekeeping transcript levels during the development of bovine preimplantation embryos Biol. Reprod. 67 2002 1465 1472
16. T.D. Schmittgen, and B.A. Zakrajsek Effect of experimental treatment on housekeeping gene expression: validation by real-time, quantitative RT-PCR J. Biochem. Biophys. Methods 46 2000 69 81
17. E. Spanakis Problems related to the interpretation of autoradiographic data on gene expression using common constitutive transcripts as controls Nucleic Acids Res. 21 1993 3809 3819
18. B.K. Steele, C. Meyers, and M.A. Ozbun Variable expression of some "housekeeping" genes during human keratinocyte differentiation Anal. Biochem. 307 2002 341 347
19. T. Suzuki, P.J. Higgins, and D.R. Crawford Control selection for RNA quantitation BioTechniques 29 2000 332 337
20. A. Szabo, C.M. Perou, M. Karaca, L. Perreard, J.F. Quackenbush, and P.S. Bernard Statistical modeling for selecting housekeeper genes Genome Biol. 5 2004 R59
21. O. Thellin, W. Zorzi, B. Lakaye, B. De Borman, B. Coumans, G. Hennen, T. Grisar, A. Igout, and E. Heinen Housekeeping genes as internal standards: use and limits J. Biotechnol. 75 1999 291 295
22. V.M. Vehaskari, J.M. Hempe, J. Manning, D.H. Aviles, and M.C. Carmichael Developmental regulation of ENaC subunit mRNA levels in rat kidney Am. J. Physiol. 274 1998 C1661 C1666
23. H. Zhong, and J.W. Simons Direct comparison of GAPDH, beta-actin, cyclophilin, and 28S rRNA as internal standards for quantifying RNA levels under hypoxia Biochem. Biophys. Res. Commun. 259 1999 523 526