A cutaneous gene therapy approach to human leptin deficiencies: Correction of the murine ob/ob phenotype using leptin-targeted keratinocyte grafts

FASEB Journal

Volumn 15, Issue 9, 2001, Pages 1529-1538

  Larcher, Fernando ^a,b   Del Rio, Marcela ^a,b   Serrano, Fernando ^a,c   Segovia, José Carlos ^a,b   Ramírez, Angel ^a   Meana, Alvaro ^a,d   Page, Angustias ^a   Abad, José Luis ^a,c   González, Manuel A ^a,c   Bueren, Juan ^a,b   Bernad, Antonio ^a,c   Jorcano, José Luis ^a,b  

^a CIEMAT   (Spain)

^b Fundacion Marceline Botín for Gene Therapy   (Spain)

^c CENTRO NACIONAL DE BIOTECNOLOGÍA   (Spain)

^d Servicio de Salud del Principado de Asturias   (Spain)

Author keywords

Diabetes; Leptin replacement; Lipodistrophy; Skin

Indexed keywords

LEPTIN;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; GENE TARGETING; GENE THERAPY; GLUCOSE BLOOD LEVEL; HUMAN; HUMAN CELL; IMMUNE DEFICIENCY; KERATINOCYTE; LIPODYSTROPHY; NONHUMAN; OBESITY; PHENOTYPE; PRIORITY JOURNAL; PROTEIN DEFICIENCY; SKIN GRAFT; TRANSGENIC MOUSE;

ANIMALS; DISEASE MODELS, ANIMAL; FEMALE; GENE THERAPY; HUMANS; KERATINOCYTES; LEPTIN; MALE; MICE; MICE, INBRED BALB C; MICE, INBRED C57BL; MICE, INBRED DBA; MICE, TRANSGENIC; OBESITY; PHENOTYPE; SKIN TRANSPLANTATION; TRANSFECTION;

EID: 17844410918     PISSN: 08926638     EISSN: None     Source Type: Journal    
DOI: 10.1096/fj.01-0082com     Document Type: Article

References (53)

1. Leptin and the regulation of body weight in mammals
2. Leptin
3. OB-Rb gene transfer to leptin-resistant islets reverses diabetogenic phenotype
4. In vivo administration of leptin activates signal transduction directly in insulin-sensitive tissues: Overlapping but distinct pathways from insulin
5. Leptin inhibits bone formation through a hypothalamic relay: A central control of bone mass
6. Leptin, the product of Ob gene, promotes angiogenesis
7. Biological action of leptin as an angiogenic factor
8. Congenital leptin deficiency is associated with severe early-onset obesity in humans
9. A leptin missense mutation associated with hypogonadism and morbid obesity
10. Effects of recombinant leptin therapy in a child with congenital leptin deficiency
11. Generalized lipodystrophy, congenital and acquired (lipoatrophy)
12. Leptin levels, beta-cell function, and insulin sensitivity in families with congenital and acquired generalized lipoatropic diabetes
13. Insulin resistance and diabetes mellitus in transgenic mice expressing nuclear SREBP-1c in adipose tissue: Model for congenital generalized lipodystrophy
14. Life without white fat: A transgenic mouse
15. Leptin reverses insulin resistance and diabetes mellitus in mice with congenital lipodystrophy
16. Increased glucose metabolism and insulin sensitivity in a mouse model of lipoatrophic diabetes by transgenic overexpression of leptin
17. Correction of obesity and diabetes in genetically obese mice by leptin gene therapy
18. Long-term correction of obesity and diabetes in genetically obese mice by a single intramuscular injection of recombinant adeno-associated virus encoding mouse leptin
19. Leptin gene therapy and daily protein administration: A comparative study in the ob/ob mouse
20. Systemic delivery of secreted protein by grafts of epidermal keratinocytes: Prospects for keratinocyte gene therapy
21. Expression of an exogenous growth hormone gene by transplantable human epidermal cells
22. Human growth hormone in the blood of athymic mice grafted with cultures of hormone-secreting human keratinocytes
23. Towards gene therapy for haemophilia B using primary human keratinocytes
24. Clonal analysis of stably transduced human epidermal stem cells in culture
25. Circulating human factor IX produced in keratin-promoter transgenic mice: A feasibility study for gene therapy of haemophilia B
26. Transgenic studies with a keratin promoter-driven growth hormone transgene: Prospects for gene therapy
27. Correction of the coagulation defect in hemophilia A mice through factor VIII expression in skin
28. Keratinocyte gene therapy for systemic diseases. Circulating interleukin 10 released from gene-transferred keratinocytes inhibits contact hypersensitivity at distant areas of the skin
29. Obese and diabetes: Two mutant genes causing diabetes-obesity syndromes in mice
30. Variation in the severity and duration of hyperglycemia in the C57BL/6-ob/ob (obese) mouse
31. Sequences 5′ of the bovine keratin 5 gene direct tissue- and cell type-specific expression of a lacZ gene in the adult and during development
32. Expression of a dominant negative mutant of epidermal growth factor receptor in the epidermis of transgenic mice elicits striking alterations in hair follicle development and skin structure
33. New method for genotyping the mouse Lep(ob) mutation, using a polymerase chain reaction assay
34. Preserved long-term repopulation and differentiation properties of hematopoietic grafts subjected to ex vivo expansion with stem cell factor and interleukin 11
35. Large surface of cultured human epithelium obtained on a dermal matrix based on live fibroblast-containing fibrin gels
36. Non-viral transfer of genes to pig primary keratinocytes. Induction of angiogenesis by composite grafts of modified keratinocytes overexpressing VEGF driven by a keratin promoter
37. New techniques for the grafting of cultured human epidermal cells onto athymic animals
38. The effect of bromocryptine-induced hypoprolactinemia on xenogeneic and allogeneic skin graft survival in a mouse model
39. Episomal vectors rapidly and stably produce high-titer recombinant retrovirus
40. Generation of retroviral vector for clinical studies using transient transfection
41. Expression of cyclin D1 in epithelial tissues of transgenic mice results in epidermal hyperproliferation and severe thymic hyperplasia
42. Constitutive expression of insulin-like growth factor-1 in epidermal basal cells of transgenic mice leads to spontaneous tumor promotion
43. Increased glucose metabolism and insulin sensitivity in transgenic skinny mice overexpressing leptin
44. Epithelial gene therapy
45. High-efficiency gene transfer and pharmacologic selection of genetically engineered human keratinocytes
46. Selection of keratinocytes transduced with the multidrug resistance gene in an in vitro skin model presents a strategy for enhancing gene expression in vivo
47. Sustainable cutaneous gene delivery
48. The importance of epidermal stem cells in keratinocyte-mediated gene therapy
49. Evidence for keratinocyte stem cells in vitro: Long term engraftment and persistence of transgene expression from retrovirus-transduced keratinocytes
50. Abnormal regulation of the leptin gene in the pathogenesis of obesity
51. Permanent coverage of large burn wounds with autologous cultured human epithelium
52. Cultivation of human keratinocyte stem cells: Current and future clinical applications
53. Treatment of 'stable' vitiligo by timed surgery and transplantation of cultured epidermal autografts