cDNA cloning, functional expression and antifungal activities of a dimeric plant defensin SPE10 from Pachyrrhizus erosus seeds

Plant Molecular Biology

Volumn 57, Issue 1, 2005, Pages 13-20

  Song, Xiaomin ^a,b   Wang, Jing ^b   Wu, Fang ^b   Li, Xu ^b   Teng, Maikun ^b   Gong, Weimin ^a,b  

^a INSTITUTE OF BIOPHYSICS   (China)

^b UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA   (China)

Author keywords

Antifugal; cDNA; Pachyrrhizus erosus; Pichia pastoris; Plant defensin

Indexed keywords

AMINO ACIDS; CLONING; DNA; FUNGI; POLYPEPTIDES; SEED; SPECTROSCOPIC ANALYSIS; YEAST;

AMINO ACID PEPTIDES; CDNA CLONING; CODONS; GENE SEQUENCES;

PLANTS (BOTANY);

ANTIFUNGAL AGENT; COMPLEMENTARY DNA; DEFENSIN; RECOMBINANT PROTEIN; VEGETABLE PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; CHEMISTRY; CIRCULAR DICHROISM; DIMERIZATION; DNA SEQUENCE; DRUG EFFECT; FUNGUS; GENE EXPRESSION; GENETICS; GROWTH, DEVELOPMENT AND AGING; IC 50; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; MOLECULAR WEIGHT; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; NUCLEOTIDE SEQUENCE; PICHIA; PLANT SEED; PROTEIN CONFORMATION; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY; SPECTROFLUOROMETRY; YAM;

AMINO ACID SEQUENCE; ANTIFUNGAL AGENTS; CIRCULAR DICHROISM; CLONING, MOLECULAR; DEFENSINS; DIMERIZATION; DNA, COMPLEMENTARY; FUNGI; GENE EXPRESSION; INHIBITORY CONCENTRATION 50; MAGNETIC RESONANCE SPECTROSCOPY; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; PACHYRHIZUS; PICHIA; PLANT PROTEINS; PROTEIN CONFORMATION; RECOMBINANT PROTEINS; SEEDS; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, AMINO ACID; SPECTROMETRY, FLUORESCENCE;

AMINO ACIDS; CLONES; FUNGI; NUCLEIC ACIDS; PEPTIDES; PLANTS; SEEDS; YEASTS;

FUNGI; PACHYRHIZUS; PICHIA PASTORIS;

EID: 17844363007     PISSN: 01674412     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11103-004-6637-y     Document Type: Article

References (29)

1. M.S. Almeida K.S. Cabral L. Nevesde Medeiros A.P. Valente F.C.L. Almeida E. Kurtenbach 2001 cDNA Cloning and heterologous expression of functional cysteine-rich antifungal protein Psd1 in the yeast Pichia pastoris Arch Biochem Biophys 395 199 207
2. J.L. Bennetzen B.D. Hall 1981 Codon selection in yeast J Biol Chem 257 3026 3031
3. C.Jr. Bloch M. Richardson 1991 A new family of small (5 kDa) protein inhibitors of insect alpha-amylases from seeds or sorghum (Sorghum bicolar (L) Moench) have sequence homologies with wheat gamma-purothionins FEBS Lett 279 101 104
4. B.P. Cammue M.F. De Bolle F.R. Terras P. Proost J. Van Damme S.B. Rees J. Vanderleyden W.F. Broekaert 1992 Isolation and characterization of a novel class of plant antimicrobial peptides form Mirabilis jalapa L. seeds J. Biol. Chem. 267 2228 2233
5. S. Cociancich A. Ghazio C. Hetru J.A. Hoffmann L. Letelliers 1993 Insect defensin, an inducible antibacterial peptide, forms voltage-dependent channels in Micrococcus luteus J. Biol. Chem. 268 19239 19245
6. G.W. De Samblanx I.J. Goderis K. Thevissen R. Raemaekers F. Fant F. Borremans D.P. Acland R.W. Osborn S. Patel W.F. Broekaert 1997 Mutational analysis of a plant defensin from radish (Raphanus sativusL.) reveals two adjacent sites important for antifungal activity J. Biol. Chem. 272 1171 1179
7. F. Fant W. Vranken W. Broekaert F. Borremans 1998 Determination of the three-dimensional solution structure of Raphanus sativus antifungal protein 1 by 1H NMR J. Mol. Biol. 279 257 270
8. C.P. Hill J. Yee M.E. Selsted D. Eisenberg 1991 The crystal structure of defensin HNP-3, an amphophilic simer, suggests mechanisms of membrane permeabilization Science 251 1481 1485
9. D.M. Hoover K.R. Rajashankar R. Blumenthal A. Puri J.J. Oppenheim O. Chertov J. Lubkowski 2000 The structure of human β-Defensin-2 shows evidence of higher order oligomerization J. Biol. Chem. 275 4232911 4232918
10. B.L. Kagan M.E. Selsted T. Ganz R.I. Lehre 1990 Antimicrobial defensin peptides from voltage-dependent ion-permeable channels in planar lipid bilayer membranes Proc. Natl. Acad. Sci. U.S.A. 87 210 214
11. P. Kraulis 1991 MolScript, a program to produce both detailed and schematic plots of protein structures J. Appl. Crystallog. 24 946 950
12. R.I. Lehrer T. Ganz D. Szklarek M.E. Selsted 1988 Modulation of the in vitro candidacidal activity of human neutrophil defensins by target cell metabolism and divalent cations J. Clin. Invest. 81 1829 1835
13. E. Mendez A. Moreno F.J. Colilla F. Pelaez G.G. Limas R. Mendez F. Soriano M. Salinas C. de Haro 1990 Primary structure and inhibition of protein synthesis in eukaryotic cell-free system of a novel thionin, gamma-horodthionin, from barley endosperm Eur. J. Biochem. 194 533 539
14. E.A. Merritt D.J. Bacon 1997 Raster3D-Photorealistic molecular graphics Methods Enzymol. 277 505 524
15. M. Moreno A. Segura F. Garcia-Olmedo 1994 Pseudothionin-St1, a potato peptide active against potato pathogens Eur. J. Biochem. 223 135 139
16. R.W. Osborn G.W. De Samblanx K. Thevissen I. Goderis S. Torrekens F. Van Leuven S. Attenborough S.B. Rees W.F. Broekaert 1995 Isolation and characterisation of plant defensins from seeds of Asteraceae, Fabaceae, Hippocastanaceae and Saxifragaceae FEBS Lett 368 257 262
17. M. Osusky G. Zhou L. Osuska R.E. Hancock W.W. Kay S. Misra 2000 Transgenic plants expressing cationic peptide chimeras exhibit broad-spectrum resistance to phytopathogens Nat. Biotechnol. 18 1162 1166
18. Z.K. Punja 2001 Genetic engineering of plants to enhance resistance to fungal pathogens - a review of progress and future prospects Can. J. Plant Pathol. 23 216 235
19. P.A. Raj A.R. Dentino 2002 Current status of defensins and their role in innate and adaptive immunity FEMS Microbiol. Lett. 206 9 18
20. D.J. Schibli H.N. Hunter V. Aseyev T.D. Starner J.M. Wiencek P.B. McCray B.F. Tack H.J. Vogel 2002 The solution structures of the human ß-Defensins lead to a better understanding of the potent bactericidal activity of HBD3 against Staphylococcus aureus J. Biol. Chem. 277 8279 8289
21. A. Segura M. Moreno A. Molina F. Garcia-Olmedo 1998 Novel defensin subfamily from spinach (Spinacia oleracea) FEBS Lett. 435 159 162
22. X. Song Z. Zhou J. Wang F. Wu W. Gong 2004 Purification, characterization and preliminary crystallographic studies of a novel plant defensin from Pachyrrhizus erosus seeds Acta Cryst. D 60 1121 1124
23. F.R. Terras H.M. Schoofs M.F. De Bolle F. Van Leuven S.B. Rees J. Vanderleyden B.P. Cammue W.F. Broekaert 1992 Analysis of two novel classes of plant antifungal proteins from radish (Raphanus sativus L.) seeds J. Biol. Chem. 267 15301 15309
24. F.R. Terras K. Eggermont V. Kovaleva N.V. Raikhel R.W. Osborn A. Kester S.B. Rees S. Torrekens F. Van Leuven J. Vanderleyden B.P. Cammue W.F. Broekaert 1995 Small cysteine-rich antifungal proteins from radish: their role in host defense Plant Cell 7 568 573
25. K. Thevissen R.W. Osborn D.P. Acland W.F. Broekaert 1997 Specific, high affinity binding sites for an antifungal plant defensin onNeurospora crassa hyphaeand microsomal membranes J. Biol. Chem. 272 32176 32181
26. K. Thevissen A. Ghazi G.W. De Samblanx C. Brownleei R.W. Osborn W.F. Broekaert 1996 Fungal Membrane Responses Induced by Plant Defensins and Thionins J Biol Chem 271 15018 15025
27. J.D. Thompson D.G. Higgins T.J. Gibson 1994 CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice Nucleic Acids Res. 22 4673 4680
28. W.C. Wimley M.E. Selsted S.H. White 1994 Interactions between human defensins and lipid bilayers: evidence for formation of multimeric pores Protein Sci. 3 1362 1373
29. D. Yang A. Biragyn L.W. Kwak J.J. Oppenheim 2002 Mammalian defensins in immunity: more than just microbicidal Trends Immunol., 23 291 296