Construction and real-time RT-PCR validation of Candida albicans PALS-GFP reporter strains and their use in flow cytometry analysis of ALS gene expression in budding and filamenting cells

Microbiology

Volumn 151, Issue 4, 2005, Pages 1051-1060

  Green, Clayton B ^a   Zhao, Xiaomin ^a   Yeater, Kathleen M ^a   Hoyer, Lois L ^a  

^a UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELL SURFACE PROTEIN; GREEN FLUORESCENT PROTEIN;

ARTICLE; CANDIDA ALBICANS; CELL BUDDING; CELLULAR DISTRIBUTION; CONTROLLED STUDY; CULTURE MEDIUM; FLOW CYTOMETRY; FUNGAL STRAIN; FUNGUS GROWTH; GENETIC TRANSCRIPTION; GROWTH CURVE; INOCULATION; MICROSCOPY; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; REPORTER GENE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; VALIDATION PROCESS; WILD TYPE; YEAST CELL;

BASE SEQUENCE; CANDIDA ALBICANS; DNA PRIMERS; DNA, FUNGAL; FLOW CYTOMETRY; FUNGAL PROTEINS; GENE EXPRESSION; GENES, FUNGAL; GENES, REPORTER; GREEN FLUORESCENT PROTEINS; PROMOTER REGIONS (GENETICS); RECOMBINANT FUSION PROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION;

CANDIDA ALBICANS;

EID: 17644425256     PISSN: 13500872     EISSN: None     Source Type: Journal    
DOI: 10.1099/mic.0.27696-0     Document Type: Article

References (28)

1. Barelle, C. J., Manson, C. L., MacCallum, D. M., Odds, F. C., Gow, N. A. R. & Brown, A. J. P. (2004). GFP as a quantitative reporter of gene regulation in Candida albicans. Yeast 21, 333-340.
2. Calderone, R. A. & Braun, P. C. (1991). Adherence and receptor relationships of Candida albicans. Microbiol Rev 55, 1-20.
3. Chandra, J., Kuhn, D. M. Mukherjee P. K., Hoyer, L. L., McCormick, T. & Ghannoum, M. A. (2001). Biofilm formation by the fungal pathogen Candida albicans: development, architecture and drug resistance. J Bacteriol 183, 5385-5394.
4. Collart, M. A. & Oliviero, S. (1993). Preparation of yeast RNA. In Current Protocols in Molecular Biology, vol. 2, pp. 13.12.1-13.12.5. Edited by F. M. Ausubel. New York: Wiley.
5. Cormack, B. P., Bertram, G., Egerton, M., Gow, N. A., Falkow, S. & Brown, A. J. (1997). Yeast-enhanced green fluorescent protein (yEGFP) a reporter of gene expression in Candida albicans. Microbiology 143, 303-311.
6. Fonzi, W. A. & Irwin, M. Y. (1993). Isogenic strain construction and gene mapping in Candida albicans. Genetics 134 717-728.
7. Fu, Y., Ibrahim, A. S., Sheppard, D. C., Chen, Y. C., French, S. W., Cutler, J. E., Filler, S. G. & Edwards, J. E., Jr (2002). Candida albicans Als1p: an adhesin that is a downstream effector of the EFG1 filamentation pathway. Mol Microbiol 44, 61-72.
8. Gaur, N. K. & Klotz, S. A. (1997). Expression, cloning, and characterization of a Candida albicans gene, ALA1, that confers adherence properties upon Saccharomyces cerevisiae for extracellular matrix proteins. Infect Immun 65, 5289-5294.
9. Gerami-Nejad, M., Berman, J. & Gale, C. G. (2001). Cassettes for PCR-mediated construction of green, yellow, and cyan fluorescent protein fusions in Candida albicans. Yeast 18, 859-864.
10. Green, C. B., Cheng, G., Chandra, J., Mukherjee, P., Ghannoum, M. A. & Hoyer, L. L. (2004). RT-PCR detection of Candida albicans ALS gene expression in the reconstituted human epithelium (RHE) model of oral candidiasis and in model biofilms. Microbiology 150, 267-275.
11. Heid, C. A., Stevens, J., Livak, K. J. & Williams, P. M. (1996). Real-time quantitative PCR. Genome Res 6, 986-994.
12. Hicks, J. B. & Herskowitz, I. (1976). Interconversion of yeast mating types. I. Direct observations of the action of the homothallism (HO) gene. Genetics 83, 245-258.
13. Hoyer, L. L. (2001). The ALS gene family of Candida albicans. Trends Microbiol 9, 176-180.
14. Hoyer, L. L., Scherer, S., Shatzman, A. R. & Livi, G. P. (1995). Candida albicans ALS1: domains related to a Saccharomyces cerevisiae sexual agglutinin separated by a repeating motif. Mol Microbiol 15, 39-54.
15. Hoyer, L. L., Payne, T. L., Bell, M., Myers, A. M. & Scherer, S. (1998a). Candida albicans ALS3 and insights into the nature of the ALS gene family. Curr Genet 33, 451-459.
16. Hoyer, L. L., Payne, T. L. & Hecht, J. E. (1998b). Identification of Candida albicans ALS2 and ALS4 and localization of Als proteins to the fungal cell surface. J Bacteriol 180, 5334-5343.
17. Jones, T., Federspiel, N. A., Chibana, H. & 9 other authors (2004). The diploid genome sequence of Candida albicans. Proc Natl Acad Sci U S A 101, 7329-7334.
18. Kuhn, D. M., Chandra, J., Mukherjee, P. K. & Ghannoum, M. A. (2002). Comparison of biofilms formed by Candida albicans and Candida parapsilosis on bioprosthetic surfaces. Infect Immun 70, 878-888.
19. Mann, J. L. (1983). Autofluorescence of fungi: an aid to detection in tissue sections. Am J Clin Pathol 79, 587-590.
20. Murad, A. M., Lee, P. R., Broadbent, I. D., Barelle, C. J. & Brown, A. J. (2000). CIp10, an efficient and convenient integrating vector for Candida albicans. Yeast 16, 325-327.
21. Oh, S.-H., Cheng, G., Nuessen, J. A., Jajko, R., Yeater, K. M., Zhao, X., Pujol, C., Soll, D. R. & Hoyer, L. L. (2005). Functional specificity of Candida albicans Als3p proteins and clade specificity of ALS3 alleles discriminated by the number of copies of the tandem repeat sequence in the central domain. Microbiology 151, 673-681.
22. Porta, A., Ramon, A. M. & Fonzi, W. A. (1999). PRR1 a homolog of Aspergillus nidulans palF, control pH-dependent gene expression and filamentation in Candida albicans. J Bacteriol 181, 7516-7523.
23. Sheppard, D. C., Yeaman, M. R., Welch, W. H. & 8 other authors (2004). Functional and structural diversity in the Als protein family of Candida albicans. J Biol Chem 279, 30480-30489.
24. Wojciechowicz, D., Lu, C. F., Kurjan, J. & Lipke, P. (1993). Cell surface anchorage and ligand-binding domains of the Saccharomyces cerevisiae cell adhesion protein α-agglutinin, a member of the immunoglobulin superfamily. Mol Cell Biol 13, 2554-2563.
25. Zhang, N. Harrex A. L., Holland, B. R., Fenton, L. E., Cannon, R. D. & Schmid, J. (2003). Sixty alleles of the ALS7 open reading frame in Candida albicans: ALS7 is a hypermutable contingency locus. Genome Res 13, 2005-2017.
26. Zhao, X., Pujol, C., Soll, D. R. & Hoyer, L. L. (2003). Allelic variation in the contiguous loci encoding Candida albicans ALS5, ALS1 and ALS9. Microbiology 149, 2947-2960.
27. Zhao, X., Oh, S.-H., Cheng, G., Green, C. B., Nuessen, J. A., Yeater, K., Leng, R. P., Brown, A. J. P. & Hoyer, L. L. (2004). ALS3 and ALS8 represent a single locus that encodes a Candida albicans adhesin; functional comparison between Als3p and Als1p. Microbiology 150, 2415-2428.
28. Zhao, X., Oh, S.-H., Yeater, K. M. & Hoyer, L. L. (2005). Analysis of the Candida albicans Als2p and Als4p adhesins suggests the potential for compensatory function within the Als family. Microbiology 151 (in press).