Expression, mutagenesis, and characterization of recombinant low-potential cytochrome c550 of photosystem II

Biochemistry

Volumn 44, Issue 16, 2005, Pages 6092-6100

  Andrews, Heather ^a   Li, Zhaoliang ^a   Altuve Blanco, Adriana ^b   Rivera, Mario ^b   Burnap, Robert L ^a  

^a OKLAHOMA STATE UNIVERSITY   (United States)

^b UNIVERSITY OF KANSAS   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; CHARACTERIZATION; ESCHERICHIA COLI; HYDROGEN BONDS; MUTAGENESIS; SPECTROSCOPIC ANALYSIS;

CYTOCHROMES; PROTEIN FOLDS; REDOX POTENTIALS; WILD-TYPE PROTEINS;

PROTEINS;

BETAINE; CYTOCHROME C; CYTOCHROME C550; GALACTOSIDE; HEME DERIVATIVE; ISOPROPYL BETA DETRO THIOGALACTOSIDE; MUTANT PROTEIN; PROPIONIC ACID DERIVATIVE; RECOMBINANT PROTEIN; SUCROSE; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL GROWTH; CONTROLLED STUDY; CYTOPLASM; ESCHERICHIA COLI; HETEROLOGOUS EXPRESSION; HYDROGEN BOND; LINKAGE ANALYSIS; MOLECULAR INTERACTION; MUTAGENESIS; MUTATION; NONHUMAN; OXIDATION REDUCTION POTENTIAL; PHOTOSYSTEM II; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN SECRETION; SITE DIRECTED MUTAGENESIS; SPECTROSCOPY; SPECTRUM; SYNECHOCYSTIS; WILD TYPE;

BACTERIAL PROTEINS; BASE SEQUENCE; CYTOCHROME C GROUP; DNA, BACTERIAL; GENE EXPRESSION; GENES, BACTERIAL; HEME; MEMBRANE POTENTIALS; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; OXIDATION-REDUCTION; PHOTOSYSTEM II PROTEIN COMPLEX; PROTEIN CONFORMATION; RECOMBINANT PROTEINS; SPECTROPHOTOMETRY; SYNECHOCYSTIS;

CYANOBACTERIA; SYNECHOCYSTIS;

EID: 17644423964     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi0500473     Document Type: Article

References (36)

1. Dolla, A., Blanchard, L., Guerlesquin, F., and Bruschi, M. (1994) The protein moiety modulates the redox potential in cytochromes c, Biochimie 76, 471-479.
2. Barker, P. D., and Ferguson, S. J. (1999) Still a puzzle: why is haem covalently attached in c-type cytochromes?, Struct. Fold Des. 7, R281-R290.
3. Holten, R. W., and Meyers, J. (1963) Cytochromes of a blue-green algae: extraction of a c-type with a strongly negative redox potential, Science 142, 234-235.
4. Vrettos, J. S., Reifler, M. J., Kievit, O., Lakshmi, K. V., de Paula, J. C., and Brudvig, G. W. (2001) Factors that determine the unusually low reduction potential of cytochrome c550 in cyano-bacterial photosystem II, J. Biol. Inorg. Chem. 6, 708-716.
5. Roncel, M., Boussac, A., Zurita, J. L., Bottin, H., Sugiura, M., Kirilovsky, D., and Ortega, J. M. (2003) Redox properties of the photosystem II cytochromes b559 and c550 in the cyanobacterium Thermosynechococcus elongatus, J. Biol. Inorg. Chem. 8, 206-216.
6. Navarro, J. A., Hervas, M., De la Cerda, B., and De la Rosa, M. A. (1995) Purification and physicochemical properties of the low potential cytochrome C549 from the cyanobacterium Synechocystis sp. PCC 6803, Arch. Biochem. Biophys. 3186, 46-52.
7. Cho, Y. S., Wang, Q. J., Krogmann, D., and Whitmarsh, J. (1999) Extinction coefficients and midpoint potentials of cytochrome c(6) from the cyanobacteria Arthrospira maxima, Microcystis aeruginosa, and Synechocystis 6803, Biochim. Biophys. Acta 1413, 92-97.
8. Sawaya, M. R., Krogmann, D. W., Serag, A., Ho, K. K., Yeates, T. O., and Kerfeld, C. A. (2001) Structures of cytochrome c-549 and cytochrome c6 from the cyanobacterium Arthrospira maxima, Biochemistry 40, 9215-9225.
9. Mao, J., Hauser, K., and Gunner, M. R. (2003) How cytochromes with different folds control heme redox potentials, Biochemistry 42, 9829-9840.
10. 5, Faraday Discuss. 116, 221-234.
11. Thony-Meyer, L. (2002) Cytochrome c maturation; a complex pathway for a simple task?, Biochem. Soc. Trans. 30, 633-638.
12. Schulz, H., Fabianek, R. A., Pellicioli, E. C., Hennecke, H., and Thony-Meyer, L. (1999) Heme transfer to the heme chaperone CcmE during cytochrome c maturation requires the CcmC protein, which may function independently of the ABC-transporter Cc-mAB, Proc. Natl. Acad. Sci. U.S.A. 96, 6462-6467.
13. Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd ed., Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
14. Cho, Y. S., Pakrasi, H. B., and Whitmarsh, J. (2000) Cytochrome cM from synechocystis 6803. Detection in cells, expression in Escherichia coli, purification and physical characterization, Eur. J. Biochem. 267, 1068-1074.
15. Ho, K. K., Ulrich, E. L., Krogmann, D. W., and Gomez-Lojero, C. (1979) Isolation of photosynthetic catalysts from cyanobacteria, Biochim. Biophys. Acta 545, 236-248.
16. Stankovich, M. T. (1980) An anaerobic spectroelectrochemical cell for studying the spectral and redox properties of flavoproteins, Anal. Biochem. 109, 295-308.
17. Bourot, S., Sire, O., Trautwetter, A., Touze, T., Wu, L. F., Blanco, C., and Bernard, T. (2000) Glycine betaine-assisted protein folding in a lysA mutant of Escherichia coli, J. Biol. Chem. 275, 1050-1056.
18. Sosa-Peinado, A., Mustafi, D., and Makinen, M. W. (2000) Overexpression and biosynthetic deuterium enrichment of TEM-1 beta-lactamase for structural characterization by magnetic resonance methods, Protein Expression Purif. 19, 235-245.
19. Rosell, F. I., and Mauk, A. G. (2002) Spectroscopic properties of a mitochondrial cytochrome C with a single thioether bond to the heme prosthetic group, Biochemistry 41, 7811-7818.
20. Kirilovsky, D., Roncel, M., Boussac, A., Wilson, A., Zurita, J. L., Ducruet, J. M., Bottin, H., Sugiura, M., Ortega, J. M., and Rutherford, A. W. (2004) Cytochrome c550 in the Cyanobacterium Thermosynechococcus elongatus: study of redox mutants, J. Biol. Chem. 279, 52869-52880.
21. Shen, J. R., Ikeuchi, M., and Inoue, Y. (1992) Stoichiometric association of extrinsic cytochrome c550 and 12 kDa protein with a highly purified oxygen evolving photosystem II core complex from Synechococcus vulcanus, FEBS Lett. 301, 145-149.
22. Frazao, C., Enguita, F. J., Coelho, R., Sheldrick, G. M., Navarro, J. A., Hervas, M., De la Rosa, M. A., and Carrondo, M. A. (2001) Crystal structure of low-potential cytochrome c549 from Synechocystis sp. PCC 6803 at 1.21 Å resolution, J. Biol. Inorg. Chem. 6, 324-332.
23. Thony-Meyer, L., Kunzler, P., and Hennecke, H. (1996) Requirements for maturation of Bradyrhizobium japonicum cytochrome c550 in Escherichia coli, Eur. J. Biochem. 235, 754-761.
24. Thony-Meyer, L. (2000) Haem-polypeptide interactions during cytochrome c maturation, Biochim. Biophys. Acta 1459, 316-324.
25. Tomlinson, E. J., and Ferguson, S. J. (2000) Loss of either of the two heme-binding cysteines from a class I c-type cytochrome has a surprisingly small effect on physicochemical properties, J. Biol. Chem. 275, 32530-32534.
26. Mauk, M. R., Mauk, A. G., Chen, Y. L., and Douglas, D. J. (2002) Tandem mass spectrometry of protein-protein complexes: cytochrome c-cytochrome b5, J. Am. Soc. Mass Spectrum. 13, 59-71.
27. 1H NMR spectroscopic studies of the rat outer mitochondrial membrane cytochrome b5, Biochemistry 31, 12233-12240.
28. Moore, G. R., and Pettigrew, G. W. (1990) Cytochromes c. Evolutionary, Structural, and Physiochemical Aspects, Springer, Berlin, Heidelberg, and New York.
29. Reid, L. S., Mauk, M. R., and Mauk, A. G. (1984) Role of heme propionate groups in cytochrome bs electron transfer, J. Am. Chem. Soc. 106, 2182-2185.
30. Rivera, M., Seetharaman, R., Girdhar, D., Wirtz, M., Zhang, X., Wang, X., and White, S. (1998) The reduction potential of cytochrome b5 is modulated by its exposed heme edge, Biochemistry 37, 1485-1494.
31. Kerfeld, C. A., and Krogmann, D. (1998) Photosynthetic cytochromes c in cyanobacteria, algae, and plants, Annu. Rev. Plant Physiol. Plant Mol. Biol. 49, 397-425.
32. Cutler, R. L., Davies, A. M., Creighton, S., Warshel, A., Moore, G. R., Smith, M., and Mauk, A. G. (1989) Role of arginine-38 in regulation of the cytochrome c oxidation-reduction equilibrium, Biochemistry 28, 3188-3197.
33. Kassner, R. J. (1972) Effects of nonpolar environments on the redox potentials of heme complexes, Proc. Natl. Acad. Sci. U.S.A. 69, 2263-2267.
34. Raphael, A. L., and Gray, H. B. (1989) Axial ligand replacement in horse heart cytochrome c by semisynthesis, Proteins 6, 338-340.
35. Miller, G. T., Zhang, B., Hardman, J. K., and Timkovich, R. (2000) Converting a c-type to a b-type cytochrome: Met61 to His61 mutant of Pseudomonas cytochrome c-551, Biochemistry 39, 9010-9017.
36. 550 of photosystem II in Synechocystis sp. PCC6803, Biochemistry 43, 14161-14170.