Role of Cys-295 on subunit interactions and allosteric regulation of phosphofructokinase-2 from Escherichia coli

FEBS Letters

Volumn 579, Issue 11, 2005, Pages 2313-2318

  Caniuguir, Andrés ^a   Cabrera, Ricardo ^a   Báez, Mauricio ^a   Vásquez, Claudio C ^b   Babul, Jorge ^a   Guixé, Victoria ^a  

^a UNIVERSITY OF CHILE   (Chile)

^b UNIVERSIDAD DE SANTIAGO DE CHILE   (Chile)

Author keywords

Allosteric regulation; Escherichia coli; Phosphofructokinase; SH group; Subunit interaction

Indexed keywords

6 PHOSPHOFRUCTO 2 KINASE; ADENOSINE TRIPHOSPHATE MAGNESIUM; ALANINE; CYSTEINE; OLIGOMER; PHENYLALANINE; TETRAMER;

ALLOSTERISM; ARTICLE; CONTROLLED STUDY; DIMERIZATION; ENZYME ACTIVITY; ESCHERICHIA COLI; GENE MUTATION; KINETICS; NONHUMAN; PARAMETER; PRIORITY JOURNAL; PROTEIN FUNCTION; PROTEIN INTERACTION; PROTEIN STABILITY; PROTEIN STRUCTURE; WILD TYPE;

EID: 17644363680     PISSN: 00145793     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.febslet.2005.02.078     Document Type: Article

References (18)

1. Y. Shirakihara, and P. Evans Crystal structure of the complex of phosphofructokinase from Escherichia coli with its reaction products J. Mol. Biol. 204 1988 973 994
2. D. Blangy, H. Buc, and J. Monod Kinetics of the allosteric interactions of phosphofructokinase from Escherichia coli J. Mol. Biol. 31 1968 13 35
3. W.R. Rypniewski, and P.R. Evans Crystal structure of unliganded phosphofructokinase from Escherichia coli J. Mol. Biol. 207 1989 805 821
4. J. Babul Phosphofructokinases from Escherichia coli. Purification and characterization of the nonallosteric isozyme J. Biol. Chem. 253 1978 4350 4355
5. T. Schirmer, and P.R. Evans Structural basis of the allosteric behaviour of phosphofructokinase Nature 343 1990 140 145
6. G. Le Bras, I. Auzat, and J.R. Garel Tetramer-dimer equilibrium of phosphofructokinase and formation of hybrid tetramers Biochemistry 34 1995 13203 13210
7. I. Auzat, G. Le Bras, and J.R. Garel Hypercooperativity induced by interface mutations in the phosphofructokinase from Escherichia coli J. Mol. Biol. 246 1995 248 253
8. V. Guixé, and J. Babul Effect of ATP on phosphofructokinase-2 from Escherichia coli. A mutant enzyme altered in the allosteric site for MgATP J. Biol. Chem. 260 1985 11001 11005
9. -2 Biochemistry 37 1998 13269 13275
10. V. Guixé, and J. Babul Influence of ligands on the aggregation of the normal and mutant forms of phosphofructokinase 2 of Escherichia coli Arch. Biochem. Biophys. 264 1988 519 524
11. V. Guixé Chemical modification of SH groups of E. coli phosphofructokinase-2 induces subunit dissociation: monomers are inactive but preserve ligand binding properties Arch. Biochem. Biophys. 376 2000 313 319
12. M. Báez, P.H. Rodríguez, J. Babul, and V. Guixé Structural and functional roles of cysteines 238 and 295 in Escherichia coli phosphofructokinase-2 Biochem. J. 376 2003 277 283
13. F. Daldal Molecular cloning of the gene for phosphofructokinase-2 of Escherichia coli and the nature of a mutation, pfkB1, causing a high level of the enzyme J. Mol. Biol. 168 1983 285 305
14. S. Tabor, and C.C. Richardson A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes Proc. Nat. Acad. Sci. USA 82 1985 1074 1078
15. R. Cabrera, H. Fischer, S. Trapani, A.F. Craievich, R.C. Garrat, V. Guixé, and J. Babul Domain motions and quaternary packing of phosphofructokinase-2 from Escherichia coli studied by Small Angle X-ray Scattering and homology modelling J. Biol. Chem. 278 2003 12913 12919
16. J.K. Inlow, and T.O. Baldwin Mutational analysis of the subunit interface of Vibrio harveyi bacterial luciferase Biochemistry 41 2002 3906 3915
17. J.C. Torres, V. Guixé, and J. Babul A mutant phosphofructokinase produces a futile cycle during gluconeogenesis in Escherichia coli Biochem. J. 327 1997 675 684
18. M.A. Vargo, L. Nguyen, and R. Colman Subunit interface residues of glutathione S-transferase A1-1 that are important in the monomer-dimer equilibrium Biochemistry 43 2004 3327 3335