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Volumn 44, Issue 8, 2005, Pages 2582-2584

Photosensitizer in a molecular bowl: Steric protection enhancing the photonuclease activity of copper(II) scorpionates

Author keywords

[No Author keywords available]

Indexed keywords

COPPER COMPLEX; DNA; LIGAND; OXYGEN; PHENAZINE DERIVATIVE; PHOTOSENSITIZING AGENT; QUINOXALINE DERIVATIVE;

EID: 17444402456     PISSN: 00201669     EISSN: None     Source Type: Journal    
DOI: 10.1021/ic050085a     Document Type: Article
Times cited : (29)

References (33)
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    • note
    • 17 The reactions were carried out under illuminated conditions using a monochromatic UV lamp of 365-nm wavelength (12 W), a CW He-Ne laser of 632.8-nm wavelength (3 mW power, Scientifica-Cook Ltd., U.K.), and a pulsed ruby laser of 694-nm wavelength (Lumonics, 1/6 Hz, 20 ns). Eppendorf and glass vials were used for the UV- and visible-light experiments, respectively, in a dark room at 25°C using SC DNA (0.8 μL, 0.5 μg) in 50 mM tris(hydroxymethyl) methane-HCl (Tris-HCl) buffer (pH 7.2) containing 50 mM NaCl and the complex at varied concentrations. The concentration of each complex or additive corresponded to the quantity of the sample in 2 μL of stock solution that was subsequently diluted to 18 μL with buffer prior to photolysis. For anaerobic experiments, irradiation of the solutions was done under positive pressure of argon following freeze - pump - thaw cycles.
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