Failure to detect hepatitis B virus in vitreous by polymerase chain reaction

Ophthalmologica

Volumn 219, Issue 2, 2005, Pages 93-96

  Aras, Cengiz ^a,b   Ozdamar, Akif ^a   Ergin, Sevgi ^a   Ozturk, Recep ^a   Midilli, Kenan ^a   Karacorlu, Murat ^a   Ozkan, Sehirbay ^a  

^a ISTANBUL UNIVERSITY   (Turkey)

^b NONE   (Turkey)

Author keywords

Hepatitis B virus; Polymerase chain reaction; Vitreous

Indexed keywords

HEPATITIS B SURFACE ANTIGEN; VIRUS DNA; BIOLOGICAL MARKER;

ADULT; AGED; ARTICLE; CLINICAL ARTICLE; ENZYME IMMUNOASSAY; FEMALE; HEPATITIS B VIRUS; HUMAN; MALE; POLYMERASE CHAIN REACTION; VIRUS CARRIER; VIRUS DETECTION; VITREOUS BODY; BLOOD; COMPARATIVE STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; GENETICS; IMMUNOLOGY; IN VITRO STUDY; MIDDLE AGED; VIROLOGY;

ADULT; AGED; BIOLOGICAL MARKERS; BLOOD; DNA, VIRAL; ENZYME-LINKED IMMUNOSORBENT ASSAY; FEMALE; HEPATITIS B SURFACE ANTIGENS; HEPATITIS B VIRUS; HUMANS; MALE; MIDDLE AGED; POLYMERASE CHAIN REACTION; VITREOUS BODY;

EID: 17244374803     PISSN: 00303755     EISSN: None     Source Type: Journal    
DOI: 10.1159/000083267     Document Type: Article

References (18)

1. Yoffe B, Noonan CA: Progress and perspectives in human hepatitis B virus research; in Melnick JL (ed): Progress in Medical Virology. New York, Karger, 1993, vol 40, pp 107-140.
2. Fox GM, Crouse CA, Chuang EL, Pflugfelder SC, Cleary TJ, Nelson Atherton SS: Detection of herpes virus DNA in vitreous and aqueous specimens by the polymerase chain reaction. Arch Ophthalmol 1991;109:266-271.
3. Ohishi W, Shirikawa H, Kawakami Y, Kimura S, Kamiyasu M, Tazu S, Nakanishi T, Chayama K: Identification of rare polymerase variants of hepatitis B virus using a two-stage PCR with peptide nucleic acid clamping. J Med Viral 2004;72:558-565.
4. Knox MC, Chandler D, Graham AS, Margolis TP: Polymerase chain reaction-based assays of vitreous samples for the diagnosis of viral retinitis: Use in diagnostic dilemmas. Ophthalmology 1998;105:37-45.
5. Nogueira ML, Sigueira RL, Freitas N, Amorin JB, Bonjardum CA, Ferreira PC, Orefice F, Kroon EG: Detection of herpesvirus DNA by the polymerase chain reaction (PCR) in vitreous samples from patients with necrotising retinitis. J Clin Pathol 2001;54:103-106.
6. Su CS, Bowden S, Fong LP, Taylor HR: Detection of hepatitis B virus in tears by polymerase chain reaction. Arch Ophthalmol 1994;112:621-625.
7. Mack DH, Sninsky JJ: A sensitive method for the identification of uncharacterized viruses related to known virus groups: Hepadnavirus model system. Proc Natl Acad Sci 1988;85:6977-6981.
8. de Boer JH, Verhagen J, Bruinenberg M, Rothova A, de Jong PT, Baarsma GS, Van der Lelij A, Ooyman FM, Bollemeijer JG, Derhaa Kijlstra A: Serologic and polymerase chain reaction analysis of intraocular fluids in the diagnosis of infectious uveitis. Am J Ophthalmol 1996;121:650-658.
9. Boom R, Sol CJ, Salimans MM, Jansen CL, Wertheim-van Dillen PM, van der Noordaa J: Rapid and simple method for purification of nucleic acids. J Clin Microbiol 1990;28:495-503.
10. Gastaud P, Baudouin C, Ouzan D: Detection of HBs antigen, DNA polymerase activity, and hepatitis B virus DNA in tears: Relevance to hepatitis B transmission by tears. Br J Ophthalmol 1989;73:333-336.
11. Darrell RW, Jacob GB: Hepatitis B surface antigen in human tears. Arch Ophthalmol 1978;96:674-676.
12. Elsana S, Shemer-Avni Y, Sikuler E, Sarov B, Naggan L, Bar-Shany Gilon E, Yaari A, Orgel M, Frazer GM, Margalith M: Prevalence of specific IgA and IgM anti-HBc antibodies compared with HBV DNA in the sera of HBsAg carriers. J Hum Virol 1997;1:52-57.
13. Koksal I, Cetinkaya K, Aker F: Hepatitis B surface antigen in tears and aqueous humor: A comparative study of serum hepatitis B surface antigen levels. Ophthalmologica 1992;204:19-22.
14. Friberg TR, Williamson D: Hepatitis B surface antigen in human subretinal fluid. Am J Ophthalmol 1983;95:712-713.
15. Limpens J, Kijlstra A: Human vitreous fluid contains a potent inhibitor of the polymerase chain reaction. Invest Ophthalmol Vis Sci 1993;34(suppl):1056.
16. Cunha-Vaz JG, Maurice DM: The active transport of fluorescein by the retinal vessels and the retina. J Physiol 1967;191:467-486.
17. Balazs EA: Physiology of the vitreous body; in Schepens CL (ed): Importance of the Vitreous Body in Retina Surgery with Special Emphasis on Reoperations. St Louis, Mosby, 1960, pp 29-48.
18. Balazs EA: The molecular biology of the vitreous; in McPherson A (ed): New and Controversial Aspects of Retinal Detachment. New York, Harper & Row, 1968, p 3.