The isopenicillin N acyltransferases of Aspergillus nidulans and Penicillium chrysogenum differ in their ability to maintain the 40-kDa αβ heterodimer in an undissociated form

European Journal of Biochemistry

Volumn 270, Issue 9, 2003, Pages 1958-1968

  Fernández, Francisco J ^a   Cardoza, Rosa E ^b   Montenegro, Eduardo ^a   Velasco, Javier ^a   Gutiérrez, Santiago ^a   Martín, Juan F ^a  

^a UNIVERSITY OF LEÓN   (Spain)

^b NONE

Author keywords

Enzyme processing; Filamentous fungi; Gene expression; Penicillin biosynthesis

Indexed keywords

ACYLTRANSFERASE; COMPLEMENTARY DNA; DIMER; ENZYME ANTIBODY; ENZYME PRECURSOR; FUNGAL ENZYME;

AMINO ACID SEQUENCE; ARTICLE; ASPERGILLUS NIDULANS; CATALYSIS; CONTROLLED STUDY; DISSOCIATION; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME SUBUNIT; FUNGAL GENE; GEL FILTRATION; GENE EXPRESSION; IMMUNOBLOTTING; INTRON; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; PENICILLIUM CHRYSOGENUM; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PROCESSING; PROTEIN PROTEIN INTERACTION;

ACYLTRANSFERASES; ASPERGILLUS NIDULANS; DIMERIZATION; ESCHERICHIA COLI; GENE EXPRESSION PROFILING; INCLUSION BODIES; MOLECULAR WEIGHT; PENICILLIN-BINDING PROTEINS; PENICILLIUM CHRYSOGENUM; PLASMIDS; PROTEIN FOLDING; PROTEIN PROCESSING, POST-TRANSLATIONAL; TEMPERATURE;

ACREMONIUM CHRYSOGENUM; ASPERGILLUS; EMERICELLA NIDULANS; ESCHERICHIA COLI; FUNGI; PENICILLIUM; PENICILLIUM CHRYSOGENUM;

EID: 17144453678     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1033.2003.03561.x     Document Type: Article

References (38)

1. Demain, A.L. (1983) Biosynthesis of β-lactam antibiotics. In Antibiotics Containing the β-Lactam Structure, 1 (Demain, A.L. & Solomon, N.A., eds), pp. 189-228. Springer-Verlag, Berlin, Germany.
2. Martín, J.F., Ingolia, T.D. & Queener, S.W. (1990) Molecular genetics of penicillin and cephalosporin antibiotic biosynthesis. In Molecular Industrial Mycology (Leong, S.A. & Berka, R., eds), pp. 149-195. Marcel Dekker, New York, USA.
3. Queener, S.W. & Neuss, N. (1982) The biosynthesis of β-lactam antibiotics. In β-Lactam Antibiotics (Morin, E.B. & Morgan, M., eds), Vol. 3, pp. 1-81. Academic Press, London, UK.
4. Gutiérrez, S., Diez, B., Alvarez, A., Barredo, J.L. & Martín, J.F. (1991) Expression of the penDE gene of Penicillium chrysogenum encoding isopenicillin N acyltransferase in Cephalosporium acremonium: production of benzylpenicillin by the transformants. Mol. Gen. Genet. 225, 56-64.
5. Barredo, J.L., Cantoral, J.M., Alvarez, E., Díez, B. & Martín, J.F. (1989) Cloning, sequence analysis and transcriptional study of the isopenicillin N synthase of Penicillium chrysogenurn AS-P-78. Mol. Gen. Genet. 216, 91-98.
6. Barredo, J.L., van Solingen, P., Díez, B., Alvarez, E., Cantoral, J.M., Kattevilder, A., Smaal, E.B., Groenen, M.A.M., Veenstra, A.E. & Martín, J.F. (1989) Cloning and characterization of the acyl-coenzyme A: 6-aminopenicillanic-acid-acyltransferase gene of Penicillium chrysogenum. Gene 83, 291-300.
7. Carr, L.G., Skatrud, P.L., Scheetz, M.E., Queener, S.W. & Ingolia, T.D. (1986) Cloning and expression of the isopenicillin N synthetase gene from Penicillium chrysogenum. Gene 48, 257-266.
8. Díez, B., Gutiérrez, S., Barredo, J.L., van Solingen, P., van der Voort, L.H.M. & Martín, J.F. (1990) The cluster of penicillin biosynthetic genes. J. Biol. Chem. 265, 16358-16365.
9. Smith, D.J., Burnham, M.K.R., Edwards, J., Earl, A.J. & Turner, G. (1990) Cloning and heterologous expression of the penicillin biosynthetic gene cluster from Penicillium chrysogenum. Biotechnology (NY) 8, 39-41.
10. Veenstra, A,E., van Solingen, P., Huininga-Muurling, H., Koekman, B.P., Groenne, M.A.M., Smaal, E.B., Kattevilder, A., Alvarez, E., Barredo, J.L. & Martín, J.F. (1989) Cloning of penicillin biosynthetic genes. In Genetics and Molecular Biology of Industrial Microorganisms (Hershberger, C.L., Queener, S.W. & Hegeman, G., eds), pp. 262-269. American Society for Microbiology, Washington, DC, USA.
11. MacCabe, A.P., van Liempt, H., Palissa, H., Unkles, S.E., Riach, M.B.R., Pfeifer, E., von Döhren, H. & Kinghorn, J.R. (1991) δ-(1-α-aminoadipyl)-L-cysteinyl-D-valine synthetase from Aspergillus nidulans. J. Biol. Chem. 266, 12646-12654.
12. Montenegro, E., Barredo, J.L., Gutiérrez, S., Díez, B., Alvarez, E. & Martín, J.F. (1990) Cloning, characterization of the acyl-CoA: 6-amino penicillanic acid acyltransferase gene of Aspergillus nidulans and linkage to the isopenicilhn N synthase gene. Mol. Gen. Genet. 221, 322-330.
13. Ramón, D., Carramolino, L., Patiño, C., Sánchez, F. & Peñalva, M.A. (1987) Cloning and characterization of the isopenicillin N synthetase gene mediating the formation of the β-lactam ring in Aspergillus nidulans. Gene 57, 171-181.
14. Martín, J.F., Gutiérrez, S. & Demain, A.L. (1997) β-Lactams. In Fungal Biotechnology (Anke, T., ed.), pp. 91-127. Chapman & Hall GmbH, Weinheim, Germany.
15. Montenegro, E., Fierro, F., Fernández, F.J., Gutiérrez, S. & Martín, J.F. (1992) Resolution of chromosomes III and VI of Aspergillus nidulans by pulsed-field gel electrophoresis shows that the penicillin biosynthetic pathway genes pcbAB, pcbC, and penDE are clustered on chromosome VI (3.0 megabases). J. Bacteriol. 174, 7063-7067.
16. Alvarez, E., Meesschaert, B., Montenegro, E., Gutiérrez, S., Díez, B., Barredo, J.L. & Martín, J.F. (1993) The isopenicillin N acyltransferase of Penicillium chrysogenum has isopenicillin N aminohydrolase, 6-aminopenicillanic acid acyltransferase and penicillin amidase activities, all of which are encoded by the single penDE gene. Eur. J. Biochem. 215, 323-332.
17. Tobin, M.B, Baldwin, J.E., Cole, S.C.J., Miller, J.R., Skatrud, P.L. & Sutherland, J.D. (1993) The requirement for subunit interaction in the production of Penicillium chrysogenum acyl-coenzyme A: isopenicillin N acyltransferase in Escherichia coli. Gene 132, 199-206.
18. Tobin, M.B., Fleming, M.D., Skatrud, P.L. & Miller, J.R. (1990) Molecular characterization of the acyl-coenzyme A: isopenicillin N acyltransferase gene (penDE) from Penicilliurn chrysogenurn and Aspergillus nidulans and activity of recombinant enzyme in E. coli. J. Bacteriol. 172, 5908-5914.
19. Alonso, M.J., Bermejo, F., Reglero, A., Fernández-Cañón, J.M., González de Buitrago, G. & Luengo, J.M. (1988) Enzymatic synthesis of penicillins. J. Antibiot. 41, 1074-1084.
20. Alvarez, E., Cantoral, J.M., Barredo, J.L., Díez; B. & Martín, J.F. (1987) Purification to homogeneity and characterization of the acyl-CoA: 6-APA acyltransferase of Penicillium chrysogenum. Antimicrob. Agents Chemother. 31, 1675-1682.
21. Revilla, G., Ramos, R.F., López-Nieto, M.J., Alvarez, E. & Martín, J.F. (1986) Glucose represses formation of δ-(1-α-aminoadipyl)-L-cysteinyl-D-valine and isopenicillin N synthase but not penicillin acyltransferase in Penicillium chrysogenum. J. Bacteriol. 168, 947-952.
22. Bullock, W.O., Fernández, J.M. & Short, J.M. (1987) XL-1-Blue: a high efficiency plasmid transforming recA Escherichia coli strain with beta-galactosidase selection. Biotechniques 5, 376-379.
23. Yanisch-Perron, C., Vieira, J. & Messing, J. (1985) Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors. Gene 33, 103-119.
24. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage. Nature 227, 680-685.
25. Cleland, J.L., Builder, S.E., Swartz, J.R., Winkler, M., Chang, J.Y. & Wang, D.I.C. (1992) Polyethylene glycol enhanced protein refolding. Biotechnology (NY) 10, 1013-1019.
26. Tabor, S. (1990) Expression using the RNA polymerase/promoter system. In Current Protocols in Molecular Biology (Ausubel, F.A., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A. & Struhl, K., eds), pp. 16.2.1-16.2.11. Greene Publishing and Wiley-Interscience, New York, USA.
27. Dunbar, B.S. & Schwoebel, E.D. (1990) Preparation of polyclonal antibodies. Methods Enzymol. 182, 663-670.
28. Harlow, E. & Lane, D. (1988) Antibodies, a Laboratory Handbook. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA.
29. Cantoral, J.M., Gutiérrez, S., Fierro, F., Gil-Espinosa, S., van Liempt, H. & Martín, J.F. (1993) Biochemical and characterization and molecular genetics of nine mutants of Penicillium chrysogenum impaired in penicillin biosynthesis. J. Biol. Chem. 268, 737-744.
30. Fernández, F.J., Gutiérrez, S., Velasco, J., Montenegro, E., Marcos, A.T. & Martín, J.F. (1994) Molecular characterization of three loss-of-function mutations in the isopenicillin N acyltransferase gene (penDE) of Penicillium chrysogenum. J. Bacteriol. 176, 4941-4948.
31. Baker, D., Shiau, A.K. & Agard, D.A. (1993) The role of pro regions in protein folding repression in Aspergillus nidulans. Curr. Opin. Cell Biol. 5, 966-970.
32. Aplin, R.T., Baldwin, J.E., Cole, S.C., Sutherland, J.D. & Tobin, M.B. (1993) On the production of alpha, beta-heterodimeric acyl-coenzyme A: isopenicillin N-acyltransferase of Penicillium chrysogenum. Studies using a recombinant source. FEBS Lett. 19, 166-170.
33. Whiteman, P.A., Abraham, E.P., Baldwin, J.E., Fleming, M.D., Schofield, C.J., Sutherland, J.D. & Willis, A.C. (1990) Acyl coenzyme A: 6-aminopenicillanic acid acyltransferase from Penicillium chrysogenum and Aspergillus nidulans. FEBS Lett. 262, 342-244.
34. 309 on proenzyme cleavage and activity. FEMS Microbiol. Lett. 121, 39-46.
35. Kasche, V., Haufler, U., Markowsky, D., Melnyk, S., Zeich, A. & Galunsky, B. (1987) Penicillin amidase from E. coli. Enzyme heterogeneity and stability. Ann. NY Acad. Sci. 501, 97-102.
36. Vandamme, E.J. (1980) Penicillin acylases and beta-lactamases. In Economic Microbiology (Rose, H., ed.), pp. 467-522. Academic Press, New York, USA.
37. Böck, A., Wirth, R., Schmid, G., Schumacher, G., Lang, G. & Buckel, P. (1983) The penicillin acylase from Escherichia coli ATCC11105 consists of two dissimilar subunits. FEMS Microbiol. Lett. 20, 135-139.
38. Böck, A., Wirth, R., Schmid, G., Schumacher, G., Lang, G. & Buckel, P. (1983) The two subunits of penicillin acylase are processed from a common precursor. FEMS Microbiol. Lett. 20, 141-144.