3-D multispectral monitoring of living-cell signaling using confocal-imaging and FPGA processing

2004 2nd IEEE International Symposium on Biomedical Imaging: Macro to Nano

Volumn 1, Issue , 2004, Pages 680-683

  Resat, Marianne Sowa ^a   Solinsky, James C ^a   Wiley, H Steven ^a   Perrine, Ken A ^a   Seim, Thomas A ^a   Budge, Scott E ^b  

^a PACIFIC NORTHWEST NATIONAL LABORATORY   (United States)

^b Utah State University   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACOUSTIC-OPTIC TUNABLE FILTERS (AOTF); FLUORESCENCE RESONANCE ENERGY TRANSFER (FRET); HIGH PERFORMANCE COMPUTING (HPC); ONLINE ALGORITHM PROCESSORS (OLAP);

ALGORITHMS; CELLS; CHARGE COUPLED DEVICES; DATA TRANSFER; FIELD PROGRAMMABLE GATE ARRAYS; MEDICAL IMAGING; MULTISPECTRAL SCANNERS; OPTICAL FILTERS; PROGRAM PROCESSORS; RANDOM ACCESS STORAGE; REAL TIME SYSTEMS; SIGNALING;

IMAGE PROCESSING;

EID: 17144384810     PISSN: None     EISSN: None     Source Type: Conference Proceeding    
DOI: None     Document Type: Conference Paper

References (4)

1. Imaging used a 20X objective at 1μm Z-steps. Cells were stained with Alexa Fluor 488 fluorescent 13A9 antibody for the EGFR. Reconstruction used Velocity software.
2. G. W. Gordon, G. Berry, X. H. Liang, B. Levine and B. Herman, "Quantitative fluorescence resonance energy transfer measurements using fluorescence microscopy," Biophys. J. 74, 2702-2713 (1998).
3. FPGA board from Celoxica (e.g., RC-2000 w/Xilinx XC2V6000 6 MGate FPGA at 40 MHz, with six 512 KB ZBTSRAM) interfaced to a Dell PWS340 Pentium IV (e.g., 2.4 GHz, Windows 2000 5.00.2195 SP4) using Celoxica DK 2.0 SP2 (Handle-C compiler ver. 3.2.2710).
4. B. A. Scalettar, J.R. Swedlow, J. W. Sedat, and D.A. Agard, "Dispersion, aberration and deconvolution in multi-wavelength fluorescence images," Journal of Microscopy 182 (1) 50 (1996).