Loop-mediated isothermal amplification method targeting the lytA gene for detection of Streptococcus pneumoniae

Journal of Clinical Microbiology

Volumn 43, Issue 4, 2005, Pages 1581-1586

  Seki, Mitsuko ^a   Yamashita, Yoshihisa ^b   Torigoe, Hirotaka ^a   Tsuda, Hiromasa ^a   Sato, Setsuko ^a   Maeno, Masao ^a  

^a NIHON UNIVERSITY   (Japan)

^b KYUSHU UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

PRIMER DNA;

ACCURACY; ARTICLE; BACTERIAL GENE; BACTERIAL GENETICS; BACTERIAL STRAIN; BACTERIAL VIRULENCE; BACTERIUM DETECTION; BACTERIUM ISOLATION; CONTROLLED STUDY; DNA PURIFICATION; GENE AMPLIFICATION; GENE TARGETING; GENETIC CODE; INTERMETHOD COMPARISON; ISOTHERM; LYTA GENE; NONHUMAN; NUCLEIC ACID AMPLIFICATION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RELIABILITY; SENSITIVITY ANALYSIS; STREPTOCOCCUS PNEUMONIAE; TECHNIQUE; TEMPERATURE DEPENDENCE; VALIDATION PROCESS; VELOCITY;

BACTERIAL TYPING TECHNIQUES; BASE SEQUENCE; CHILD; CHILD, PRESCHOOL; DNA PRIMERS; HUMANS; MOLECULAR SEQUENCE DATA; N-ACETYLMURAMOYL-L-ALANINE AMIDASE; NUCLEIC ACID AMPLIFICATION TECHNIQUES; PNEUMOCOCCAL INFECTIONS; SENSITIVITY AND SPECIFICITY; SPECIES SPECIFICITY; STREPTOCOCCUS PNEUMONIAE;

EID: 16844368288     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.43.4.1581-1586.2005     Document Type: Article

References (23)

1. Berry, A. M., R. A. Lock, D. Hansman, and J. C. Paton. 1989. Contribution of autolysin to virulence of Streptococcus pneumonias. Infect. Immun. 57:2324-2330.
2. Brown, P. D., and S. A. Lerner. 1998. Community-acquired pneumonia. Lancet 352:1295-1302.
3. Gillespie, S. H. 1999. The role of the molecular laboratory in the investigation of Streptococcus pneumoniae infections. Semin. Respir. Infect. 14:269-275.
4. Gillespie, S. H., T. D. McHugh, H. Ayres, A. Dickens, A. Efstratiou, and G. C. Whiting. 1997. Allelic variation in Streptococcus pneumoniae autolysin (N-acetyl muramoyl-L-alanine amidase). Infect. Immun. 65:3936-3938.
5. Gillespie, S. H., C. Ullman, M. D. Smith, and V. Emery. 1994. Detection of Streptococcus pneumoniae in sputum samples by PCR. J. Clin. Microbiol. 32:1308-1311.
6. Hall, L. M. C. 1998. Application of molecular typing to the epidemiology of Streptococcus pneumoniae. J. Clin. Pathol. 51:270-274.
7. Hall, L. M., B. Duke, and G. Urwin. 1995. An approach to the identification of the pathogens of bacterial meningitis by the polymerase chain reaction. Eur. J. Clin. Microbiol. Infect. Dis. 14:1090-1094.
8. Hawn, C. V. Z., and E. Beebe. 1965. Rapid method for demonstrating bile solubility of Diplococcus pneumoniae. J. Bacteriol. 90:549.
9. Hendolin, P. H., A. Markkanen, J. Ylikoski, and J. J. Wahlfors. 1997. Use of multiplex PCR for simultaneous detection of four bacterial species in middle ear effusions. J. Clin. Microbiol. 35:2854-2858.
10. Jacoby, G. A. 1996. Antimicrobial-resistant pathogens in the 1990s. Annu. Rev. Med. 47:169-179.
11. Kawamura, Y., R. A. Whiley, S. E. Shu, T. Ezaki, and J. M. Hardie. 1999. Genetic approaches to the identification of the mitis group within the genus Streptococcus. Microbiology 145:2605-2613.
12. Lu, J.-J., C.-L. Perng, S.-Y. Lee, and C.-C. Wan. 2000. Use of PCR with universal primers and restriction endonuclease digestions for detection and identification of common bacterial pathogens in cerebrospinal fluid. J. Clin. Microbiol. 38:2076-2080.
13. Mori, Y., M. Kitao, N. Tomita, and T. Notomi. 2004. Real-time turbidimetry of LAMP reaction for quantifying template DNA. J. Biochem. Biophys. Methods 59:145-157.
14. Nagai, K., Y. Shibasaki, K. Hasegawa, T. A. Davies, M. R. Jacobs, K. Ubukata, and P. C. Appelbaum. 2001. Evaluation of PCR primers to screen for Streptococcus pneumoniae isolates and β-lactam resistance, and to detect common macrolide resistance determinants. J. Antimicrob. Chemother. 48:915-918.
15. Neeleman, C., C. H. W. Klaassen, D. M. Klomberg, H. A. de Valk, and J. W. Mouton. 2004. Pneumolysin is a key factor in misidentification of macrolide-resistant Streptococcus pneumoniae and is a putative virulence factor of S. mitis and other streptococci. J. Clin. Microbiol. 42:4355-4357.
16. Notomi, T., H. Okayama, H. Masubuchi, T. Yonekawa, K. Watanabe, N. Amino, and T. Hase. 2000. Loop-mediated isothermal amplification of DNA. Nucleic Acids Res. 28:e63.
17. Olive, D. M., and P. Bean. 1999. Principles and applications of methods for DNA-based typing of microbial organisms. J. Clin. Microbiol. 37:1661-1669.
18. Rudolph, K. M., A. J. Parkinson, C. M. Black, and L. W. Mayer. 1993. Evaluation of polymerase chain reaction for diagnosis of pneumococcal pneumonia. J. Clin. Microbiol. 31:2661-2666.
19. Salo, P., Å. Örtqvist, and M. Leinonen. 1995. Diagnosis of bacteremic pneumococcal pneumonia by amplification of pneumolysin gene fragment in serum. J. Infect. Dis. 171:479-482.
20. Wester, C. W., D. Ariga, C. Nathan, T. W. Rice, J. Pulvirenti, R. Patel, F. Kocka, J. Ortiz, and R. A. Weinstein. 2002. Possible overestimation of penicillin resistant Streptococcus pneumoniae colonization rates due to misidentification of oropharyngeal streptococci. Diagn. Microbiol. Infect. Dis. 42:263-268.
21. Whatmore, A. M., and C. G. Dowson. 1999. The autolysin-encoding gene (lytA) of Streptococcus pneumoniae displays restricted allelic variation despite localized recombination events with genes of pneumococcal bacteriophage encoding cell wall lytic enzymes. Infect. Immun. 67:4551-4556.
22. Whatmore, A. M., A. Efstratiou, A. P. Pickerill, K. Broughton, G. Woodard, D. Sturgeon, R. George, and C. G. Dowson. 2000. Genetic relationships between clinical isolates of Streptococcus pneumonias, Streptococcus oralis. and Streptococcus mitis: characterization of "atypical" pneumococci and organisms allied to S. mitis harboring S. pneumoniae virulence factor-encoding genes. Infect. Immun. 68:1374-1382.
23. Wheeler, J., R. Freeman, M. Steward, K. Henderson, M. J. Lee, N. H. Piggott, G. J. Eltringham, and A. Galloway. 1999. Detection of pneumolysin in sputum. J. Med. Microbiol. 48:863-866.