Expression, purification, crystallization and preliminary X-ray diffraction studies of the cmcl component of Streptomyces clavuligerus 7α-cephem- methoxylase

Acta Crystallographica Section D: Biological Crystallography

Volumn 60, Issue 9, 2004, Pages 1618-1621

  Lester, Diane R ^a   Öster, Linda M ^b   Svenda, Martin ^a   Andersson, Inger ^b  

^a UPPSALA UNIVERSITY   (Sweden)

^b SWEDISH UNIVERSITY OF AGRICULTURAL SCIENCES   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

STREPTOMYCES CLAVULIGERUS;

7 ALPHA CEPHEM METHOXYLASE; 7-ALPHA-CEPHEM-METHOXYLASE; BACTERIAL DNA; METHYLTRANSFERASE; MIXED FUNCTION OXIDASE; MULTIENZYME COMPLEX;

ARTICLE; BIOSYNTHESIS; CHEMISTRY; CRYSTALLIZATION; ELECTROSPRAY MASS SPECTROMETRY; ENZYMOLOGY; ESCHERICHIA COLI; METABOLISM; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN CONFORMATION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; STREPTOMYCES; X RAY DIFFRACTION;

CRYSTALLIZATION; DNA, BACTERIAL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; METHYLTRANSFERASES; MIXED FUNCTION OXYGENASES; MULTIENZYME COMPLEXES; PROTEIN CONFORMATION; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; STREPTOMYCES; X-RAY DIFFRACTION;

EID: 16644382247     PISSN: 09074449     EISSN: None     Source Type: Journal    
DOI: 10.1107/S090744490401666X     Document Type: Article

References (15)

1. Alexander, D. C. & Jensen, S. E. (1998). J. Bacteriol 180, 4068-4079.
2. Budisa, N., Steipe, B., Demange, P., Eckerskorn, C., Kellermann, J. & Huber, R. (1995). Eur. J. Biochem. 230, 788-796.
3. Collaborative Computational Project, Number 4 (1994). Acta Cryst. D50, 760-763.
4. Coque, J. J. R., Enguita, F. J., Martin, J. F. & Liras, P. (1995). J. Bacteriol. 177, 2230-2235.
5. Daoust, D. R., Onishi, H. R., Wallick, H., Hendlin, D. & Stapley, E. O. (1973). Antimicrob. Agents Chemother. 3, 254-261.
6. Enguita, F. J., Liras, P., Leitao, A. L. & Martin, J. F. (1996). J. Biol. Chem. 271, 33225-33230.
7. Hegg, E. L. & Que, L. (1997). Eur. J. Biochem. 250, 625-629.
8. Hood, J. D., Elson, A., Gilpin, M. L. & Brown, A. G. (1983). J. Chem. Soc. Chem. Commun., pp. 1187-1188.
9. Matthews, B. W. (1968). J. Mol. Biol. 33, 491-497.
10. O'Sullivan, J. & Abraham, E. P. (1980). Biochem. J. 186, 613-616.
11. O'Sullivan, J., Aplin, R. T., Stevens, C. M. & Abraham, E. P. (1979). Biochem. J. 179, 47-52.
12. Otwinowski, Z. & Minor, W. (1997). Methods Enzymol. 276, 307-326.
13. Tudzynski, B., Mihlan, M., Rojas, M. C., Linnemannstöns, P., Gaskin, P. & Hedden, P. (2003). J. Biol. Chem. 278, 28635-28643.
14. Vagin, A. & Teplyakov, A. (1997). J. Appl. Cryst. 30, 1022-1025.
15. Xiao, X., Wolfe, S. & Demain, A. L. (1991). Biochem. J. 280, 471-474.