Optimization of conditions for studies of protein unfolding by hydrogen exchange/mass spectrometry

European Journal of Mass Spectrometry

Volumn 10, Issue 2 SPEC. ISS., 2004, Pages 289-294

  Raza, A S ^a   Smith, D L ^a  

^a UNIVERSITY OF NEBRASKA LINCOLN   (United States)

Author keywords

Hydrogen exchange; Mass spectrometry; Protein folding; Transaldolase

Indexed keywords

HYDROGEN; PROTEIN; TRANSALDOLASE;

CONCENTRATION RESPONSE; CONFERENCE PAPER; DENATURATION; INCUBATION TIME; MASS SPECTROMETRY; PROTEIN FOLDING; PUBLICATION; REDUCTION;

DEUTERIUM EXCHANGE MEASUREMENT; FUNGAL PROTEINS; HYDROGEN; MASS SPECTROMETRY; PROTEIN DENATURATION; PROTEIN FOLDING; PROTEIN STRUCTURE, TERTIARY; TRANSALDOLASE; UREA;

EID: 16544384126     PISSN: 14690667     EISSN: None     Source Type: Journal    
DOI: 10.1255/ejms.631     Document Type: Conference Paper

References (18)

1. Y. Bal, T.R. Sosnick, L. Mayne and S.W Englander, "Protein folding intermediates: Native-state hydrogen exchange", Science 269, 192 (1995).
2. H. Pan and D.L. Smith, "Quaternary structure of aldolase leads to differences in its folding and unfolding intermediates", Biochemistry 42, 5713 (2003).
3. Y. Deng and D.L. Smith, "Rate and equilibrium constants for protein unfolding and refolding determined by hydrogen exchange-mass spectrometry", Anal. Biochem. 276, 150 (1999).
4. X.R. Babu, A. Moradian and D.J. Douglas, "The methanolinduced conformational transitions of beta-lactoglobulin, cytochrome c and ubiquitin at low pH: a study by electrospray Ionization mass spectrometry", J. Am. Soc. Mass Spectrom. 12, 317 (2001).
5. D. Canet, A.M. Last, P. Tito, M. Sunde, A. Spencer, D.B. Archer, C. Redfield, C.V. Robinson and C.M. Dobson, "Local cooperativity in the unfolding of an amyloldogenic valiant of human lysozyme", Nat. Struct. Biol. 9, 308 (2002).
6. D.A. Simmons and L. Konermann, "Characterization of transient protein folding intermediates during myoglobin reconstitution by time-resolved electrospray mass spectrometry with on-line isotopic pulse labeling", Biochemistry 41, 1906 (2002).
7. A. Raza, H. Pan and D.L. Smith, "FFD exchange to study protein folding", Encyclopedia of Mass Spectrometry 2, (2003).
8. Y. Deng, Z. Zhang and D.L. Smith, "Comparison of continuous and pulsed labeling amide hydrogen exchange/mass spectrometry for studies of protein dynamics", J. Am. Soc. Mass Spectrom. 10, 675 (1999).
9. J.J. Englander, J.R. Rogero and S.W. Englander, "Protein hydrogen exchange studied by the fragment separation method", Anal. Biochem. 147, 234 (1985).
10. S. Thorell, P. Gergely, Jr, K. Banki, A. Perl and G. Schneider, "The three-dimensional structure of human transaldolase", FEBS Lett. 475, 205 (2000).
11. I. Schaaff, S. Hohmann and F.K. Zimmermann, "Molecular analysis of the structural gene for yeast transaldolase", Eur. J. Biochem. 188, 597 (1990).
12. F. Lachaise, G. Martin, C. Drougard, A. Perl, M. Vuillaume, M. Wegnez, A. Sarasin and L. Daya-Grosjean, "Relationship between posttranslational modification of transaldolase and catalase deficiency in UV-sensitive repair-deficient xeroderma pigmentosum fibroblasts and SV40-transformed human cells", Free Radic. Biol. Med. 30, 1365 (2001).
13. Y. Bai, J.S. Milne, L. Mayne and S.W. Englander, "Primary structure effects on peptide group hydrogen exchange", Proteins: Struct. Funct. Genet. 17, 75 (1993).
14. G. Thévenon-Emeric, J. Kozlowski, Z. Zhang and D.L. Smith, "Determination of amide hydrogen exchange rates in peptides by mass spectrometry", Anal. Chem. 64, 2456 (1992).
15. Y. Deng and D.L. Smith, "Identification of unfolding domains in large proteins by their unfolding rates", Biochemistry 37, 6256 (1998).
16. T. Rojsajjakul, "Unfolding of alpha subunit of tryptophan synthase by hydrogen exchange/mass spectrometry", PhD thesis. University of Nebraska, Nebraska, USA (2002).
17. J. Chen and D.L. Smith, "Unfolding and disassembly of the chaperonin GroEL occurs via a tetradecameric intermediate with a folded equatorial domain", Biochemistry 39, 4250 (2000).
18. L. Wang, H. Pan and D.L. Smith, "Hydrogen exchange-mass spectrometry: optimization of digestion conditions", Mol. Cell Proteomics 1, 132 (2002).