Characterization of a noncovalent lipocalin complex by liquid chromatography/electrospray ionization mass spectrometry

Journal of Biomolecular Techniques

Volumn 15, Issue 3, 2004, Pages 208-212

  Doneanu, Catalin E ^a   Strong, Roland K ^b   Howald, William N ^a  

^a UNIVERSITY OF WASHINGTON   (United States)

^b Fred Hutchinson Cancer Research Center   (United States)

Author keywords

Electrospray ionization mass spectrometry; Lipocalin; Liquid chromatography; Noncovalent complexes; Siderocalin

Indexed keywords

LIGAND; LIPOCORTIN 2;

AMINO ACID SEQUENCE; ARTICLE; CHEMISTRY; ELECTROSPRAY MASS SPECTROMETRY; HUMAN; LIQUID CHROMATOGRAPHY; METABOLISM; METHODOLOGY; MOLECULAR GENETICS;

AMINO ACID SEQUENCE; ANNEXIN A2; CHROMATOGRAPHY, LIQUID; HUMANS; LIGANDS; MOLECULAR SEQUENCE DATA; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION;

EID: 16544376429     PISSN: 15240215     EISSN: 19434731     Source Type: Journal    
DOI: None     Document Type: Article

References (15)

1. Robinson CV, Chung EW, Kragelund BB, et al. Probing the nature of noncovalent interactions by mass spectrometry. A study of Protein-CoA ligand binding and assembly. J Am Chem Soc 1996;118:8646-8653.
2. Smith RD, Light-Wahl KJ. Perspectives-The observation of noncovalent interactions in solution by electrospray ionization mass spectrometry-Promise, pitfalls, and prognosis. Biol Mass Spectrom 1993:22:493-501.
3. Loo JA. Studying noncovalent protein complexes by electrospray ionization mass spectrometry. Mass Spectrom Rev 1997;16:1-23.
4. Loo JA. Electrospray ionization mass spectrometry: A technology for studying noncovalent macromolecular complexes. Int J Mass Spectrom 2000;200:176-186.
5. Miranker AD. Protein complexes and analysis of their assembly by mass spectrometry. Curr Opin Struct Biol 2000;10:601-606.
6. Sobott F, Robinson CV. Protein complexes gain momentum. Curr Opin Struct Biol 2002;12:729-734.
7. Robinson CV. Protein complexes take flight. Nat Struct Biol 2002;9:505-506.
8. Loo JA, Holler TP, Foltin SK, et al. Application of electrospray ionization mass spectrometry for studying human immunodeficiency virus protein complexes. Proteins 1998;2:28-37 (Suppl.)
9. Sannes-Lowery KA, Hu P, Mack DP, Mei HY, Loo JA. HIV-1 Tat peptide binding to TAR RNA by electrospray ionization mass spectrometry. Anal Chem 1997;69: 5130-5135.
10. Goetz DH, Willie ST, Armen RS, Bratt T, Borregaard N, Strong RK. Ligand preference inferred from the structure of neutrophil gelatinase associated lipocalin. Biochemistry 2000;39:1935-1941.
11. Coles M, Diercks T, Muehlenweg B, et al. The solution structure and dynamics of human neutrophil gelatinase-associated lipocalin. J Mol Biol 1999;289:139-157.
12. Goetz DH, Holmes MA, Borregaard N, Bluhm ME, Raymond KN, Strong RK. The neutrophil lipocalin NGAL is a bacteriostatic agent that interferes with siderophoremediated iron acquisition. Mol Cell 2002;10:1033-1043.
13. Bundgaard JR, Sengelov H, Borregaard N, Kjeldsen L. Molecular cloning and expression of a cDNA encoding NGAL: A lipocalin expressed in human neutrophils. Biochem Biophys Res Commun 1994;202:1468-1475.
14. Kennedy RT, Jorgenson JW. Preparation and evaluation of packed capillary liquid chromatography columns with inner diameters from 20 to 50 micrometers. Anal Chem 1989;61:1128-1135.
15. O'Brien IG, Gibson F. The structure of enterochelin and related 2,3-dihydroxy-N-benzoylserine conjugates from Escherichia coli. Biochim Biophys Acta 1970; 215:393-402.