Magnetic cell sorting purification of differentiated embryonic stem cells stably expressing truncated human CD4 as surface marker

Stem Cells

Volumn 23, Issue 4, 2005, Pages 477-482

  David, Robert ^a   Groebner, Michael ^a   Franz, Wolfgang Michael ^a  

^a LUDWIG MAXIMILIANS UNIVERSITY   (Germany)

Author keywords

CD4; Cell transplantation; ES cells; Magnetic cell sorting

Indexed keywords

CD4 ANTIGEN; COMPLEMENTARY DNA; ENHANCED GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; RNA;

ANIMAL CELL; ANTIGEN EXPRESSION; ARTICLE; CELL CULTURE; CELL DIFFERENTIATION; CELL SELECTION; CELL SORTER; CELL TRANSPLANTATION; CELL TYPE; EMBRYO; EMBRYONIC STEM CELL; FLOW CYTOMETRY; FLUORESCENCE ACTIVATED CELL SORTING; MAGNETIC CELL SORTING; NONHUMAN; PLASMID; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA SEQUENCE; STEM CELL; TISSUE ENGINEERING; XENOPUS;

ANIMALS; ANTIGENS, CD4; BIOLOGICAL MARKERS; CELL DIFFERENTIATION; CELL SEPARATION; CELLS, CULTURED; EMBRYO; EMBRYO, NONMAMMALIAN; GREEN FLUORESCENT PROTEINS; HUMANS; MAGNETICS; MICE; RECOMBINANT FUSION PROTEINS; STEM CELLS; XENOPUS;

ANIMALIA;

EID: 16444383653     PISSN: 10665099     EISSN: None     Source Type: Journal    
DOI: 10.1634/stemcells.2004-0177     Document Type: Article

References (14)

1. Lawrenz B, Schiller H, Willbold E et al. Highly sensitive biosafety model for stem-cell-derived grafts. Cytotherapy 2004;6:212-222.
2. Wakitani S, Takaoka K, Hattori T et al. Embryonic stem cells injected into the mouse knee joint form teratomas and subsequently destroy the joint. Rheumatology (Oxford) 2003;42:162-165.
3. Conley BJ, Young JC, Trounson AO et al. Derivation, propagation and differentiation of human embryonic stem cells. Int J Biochem Cell Biol 2004;36:555-567.
4. Rippon HJ, Bishop AE. Embryonic stem cells. Cell Prolif 2004;37:23-34.
5. Muller M, Fleischmann BK, Seibert S et al. Selection of ventricular-like cardiomyocytes from ES cells in vitro. FASEB J 2000;14:2540-2548.
6. Kudriavtsev BN, Anatskaia OV, Nilova VK et al. Interconnection of parameters of the mitochondrial and myofibrillar apparatus of cardiomyocytes and ploidy and hypertrophy in certain mammalian species, differing in body mass [in Russian]. Tsitologiia 1997;39:946-964.
7. Klug MG, Soonpaa MH, Koh GY et al. Genetically selected cardiomyocytes from differentiating embryonic stem cells form stable intracardiac grafts. J Clin Invest 1996;98:216-224.
8. Zandstra PW, Bauwens C, Yin T et al. Scalable production of embryonic stem cell-derived cardiomyocytes. Tissue Eng 2003;9:767-778.
9. Zweigerdt R, Burg M, Willbold E et al. Generation of confluent cardiomyocyte monolayers derived from embryonic stem cells in suspension: a cell source for new therapies and screening strategies. Cytotherapy 2003;5:399-413.
10. Rupp RA, Snider L, Weintraub H. Xenopus embryos regulate the nuclear localization of XMyoD. Genes Dev 1994;8:1311-1323.
11. David R, Joos TO, Dreyer C. Anteroposterior patterning and organogenesis of Xenopus laevis require a correct dose of germ cell nuclear factor (xGCNF). Mech Dev 1998;79:137-152.
12. Miltenyi S, Muller W, Weichel W et al. High gradient magnetic cell separation with MACS. Cytometry 1990;11:231-238.
13. Siebenkotten G, Petry K, Behrens-Jung U et al. Employing surface markers for the selection of transfected cells. In: Recktenwald D, Radbruch A, eds. Cell Separation: Methods and Applications. New York: Marcel Dekker, 1998:271-281.
14. Gaines P, Wojchowski DM. pIRES-CD4t, a dicistronic expression vector for MACS- or FACS-based selection of transfected cells. Biotechniques 1999;26:683-638.