A simple method for cloning the complete begomovirus genome using the bacteriophage φ29 DNA polymerase

Journal of Virological Methods

Volumn 116, Issue 2, 2004, Pages 209-211

  Inoue Nagata, Alice K ^a   Albuquerque, Leonardo C ^a,b   Rocha, Wesley B ^a,b   Nagata, Tatsuya ^b  

^a EMBRAPA CERRADOS   (Brazil)

^b UNIVERSIDADE CATÓLICA DE BRASÍLIA   (Brazil)

Author keywords

Circular DNA; Geminiviridae; Rolling circle

Indexed keywords

CIRCULAR DNA; DNA POLYMERASE; ENDONUCLEASE;

ARTICLE; BACTERIOPHAGE; DNA EXTRACTION; DNA SEQUENCE; DNA VIRUS; GENE AMPLIFICATION; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; VIRUS GENOME; VIRUS INFECTION;

BEGOMOVIRUS; DNA VIRUSES; GEMINIVIRIDAE;

EID: 1642523843     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2003.11.015     Document Type: Article

References (10)

1. Dellaporta S.L., Hicks J.B. A plant DNA minipreparations. Version II. Plant Mol. Biol. Rep. 1:1983;19-21.
2. Gilbertson R.L., Faria J.C., Hanson S.F., Morales F.J., Ahlquist P., Maxwell D.P., Russel D.R. Cloning of the complete DNA genomes of four bean-infecting geminiviruses and determining their infectivity by electric discharge particle acceleration. Phytopathology. 81:1991;980-985.
3. Inoue-Nagata A.K., Nagata T., Miranda J.D.R., Bezerra I.C., Ribeiro S.G., de Ávila A.C. Expression of the coat protein of tomato infecting geminivirus in Escherichia coli for serological studies. Virus Rev. Res. 4:1999;151.
4. Inoue-Nagata A.K., Nagata T., Ribeiro S.G., Bezerra I.C., Giordano L.B., de Ávila A.C. Comparison of the coat protein cistron of geminiviruses from distinct hosts. Braz. Phytopathol. 26:2001;527.
5. Nagata, T., Inoue-Nagata, A.K., de Ávila A.C., Giordano, L.B. Print-capture PCR for detection of tomato begomovirus from plants and whiteflies, Braz. Phytopathol., in press.
6. Patel V.P., Rojas M.R., Paplomatas E.J., Gilbertson R.L. Cloning biologically active geminivirus DNA using PCR and overlapping primers. Nucleic Acids Res. 11:1993;1325-1326.
7. Rojas M.R., Gilbertson R.L., Russel D.R., Maxwell D.P. Use of degenerate primers in the polymerase chain reaction to detect whitefly-transmited geminiviruses. Plant Dis. 77:1993;340-347.
8. Sambrook, J., Fritsch, E.F., Maniatis, T., 1989. Molecular Cloning. A Laboratory Manual, second ed. Cold Spring Harbor Laboratory Press, New York.
9. Srivastava K.M., Hallan V., Raizada R.K., Chandra G., Singh B.P., Sane P.V. Molecular cloning of Indian tomato leaf curl virus genome following a simple method of concentrating the supercoiled replicative from of viral DNA. J. Virol. Methods. 51:1995;297-304.
10. Van Regenmortel, M.H.V., Fauquet, C.M., Bishop, D.H.L., Castens, E., Estes, M.K., Lemon, S., Maniloff, J., Mayo, J.A., McGeoch, D.J., Pring, C.R., Wickner, R. (Eds.), 2000. Virus Taxonomy. Seventh Report of the International Committee on the Taxonomy of Viruses. Academic Press, New York, 1121 pp.