A method for acetylcholinesterase staining of brain sections previously processed for receptor autoradiography

Biotechnic and Histochemistry

Volumn 79, Issue 1, 2004, Pages 11-16

  Lim, Miranda M ^a   Hammock, E A D ^a   Young, L J ^a  

^a EMORY UNIVERSITY   (United States)

Author keywords

Acetylcholinesterase; AChE; Amygdala; Brain; Neuroanatomy; Receptor autoradiography; Thiocholine method

Indexed keywords

ACETYLCHOLINESTERASE; BRAIN RECEPTOR; THIOCHOLINE;

ANALYTIC METHOD; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; AUTORADIOGRAPHY; BRAIN REGION; CONTROLLED STUDY; FEMALE; IMMUNOHISTOCHEMISTRY; ISOTOPE LABELING; MALE; NEUROANATOMY; NISSL STAINING; NONHUMAN; PROTEIN LOCALIZATION; RECEPTOR BINDING; VOLE;

ACETYLCHOLINESTERASE; ANIMALS; ARVICOLINAE; AUTORADIOGRAPHY; BRAIN; FEMALE; MALE; RECEPTORS, CHOLINERGIC; STAINING AND LABELING;

EID: 1642492885     PISSN: 10520295     EISSN: None     Source Type: Journal    
DOI: 10.1080/10520290410001671344     Document Type: Article

References (26)

1. Emson PC, Paxinos G, Le Gal La Salle G, Ben-Ari Y, Silver A (1979) Choline acetyltransferase and acetylcholinesterase containing projections from the basal forebrain to the amygdaloid complex of the rat. Brain Res 165: 271-282.
2. Garrett B, Geneser FA (1993) Diversity of relative intensities of acetylcholinesterase staining within the laminae of the visual cortex of four mammals. Brain Res. 609: 341-346.
3. Hedreen JC, Bacon SJ, Price DL (1985) A modified histochemical technique to visualize acetylcholinesterase-containing axons. J. Histochem Cytochem. 33: 134-140.
4. Insel TR, Shapiro LE (1992) Oxytocin receptor distribution reflects social organization in monogamous and polygamous voles. Proc. Natl. Acad. Sci USA 89: 5981-5985.
5. Insel TR, Young LJ (2001) The neurobiology of attachment. Nat. Rev Neurosci. 2: 129-136.
6. Insel TR, Wang ZX, Ferris CF (1994) Patterns of brain vasopressin receptor distribution associated with social organization in microtine rodents. J. Neurosci. 14: 5381-5392.
7. Karnovsky MJ, Roots L (1964) A "direct-coloring" thiocholine method for cholinesterases. J. Histochem Cytochem. 12: 219-221.
8. Kiernan JA (1964) Carboxylic esterases of the hypothalamus and neurohypophysis of the hedgehog. J. R. Microsc Soc. 83: 297-306.
9. Koelle GB, Friedenwald JS (1949) A histochemical method for localizing cholinesterase activity. Proc. Soc. Exp Biol. 70: 612-622.
10. Kuhar MJ, Lloyd DG, Appel N, Loats HL (1991) Imaging receptors by autoradiography: computer-assisted approaches. J. Chem Neuroanat. 4: 319-327.
11. Lim MM, Murphy AZ, Young LJ (2004) Ventral striato-pallidal oxytocin and vasopressin V1a receptors in the monogamous prairie vole (Microtus ochrogaster), J. Comp. Neurol. 468: 555-570.
12. Lim MM, Nair HP, Hammock EAD, Young LJ (2003b) Species differences in CRFR1 and CRFR2 in prairie and meadow voles predict individual differences in social behavior. In: Society for Neuroscience. New Orleans, LA: Abstract Viewer/Itinerary Planner CD-ROM; Washington, DC.
13. Moss MB, Rosene DL (1984) A perfusion-fixation procedure for the concurrent demonstration of Timm's, horseradish peroxidase (HRP), and acetylcholinesterase (AChE) histochemistry. J. Histochem Cytochem. 32: 1113-1116.
14. Palacios JM, Niehoff DL, Kuhar MJ (1981) Receptor autoradiography with tritium-sensitive film: potential for computerized densitometry. Neurosci Lett. 25: 101-105.
15. Paxinos G, Watson C (1998) The Rat Brain in Stereotaxic Coordinates. 4th ed. Academic Press, San Diego, CA.
16. Paxinos G, Franklin KBJ (2001) The Mouse Brain in Stereotaxic Coordinates. 2nd ed. Academic Press, San Diego, CA.
17. Paxinos G, Watson CR, Emson PC (1980) AChE-stained horizontal sections of the rat brain in stereotaxic coordinates. J. Neurosci Methods 3: 129-149.
18. Pert CB, Kuhar MJ, Snyder SH (1975) Autoradiographic localization of the opiate receptor in rat brain. Life Sci. 16: 1849-1853.
19. Pert CB, Kuhar MJ, Snyder SH (1976) Opiate receptor: autoradiographic localization in rat brain. Proc. Natl. Acad. Sci. USA 73: 3729-3733.
20. Sanchez MM, Young LJ, Plotsky PM, Insel TR (1999) Autoradiographic and in situ hybridization localization of corticotropin-releasing factor 1 and 2 receptors in nonhuman primate brain. J. Comp Neurol. 408: 365-377.
21. van Ooteghem SA, Shipley MT (1984) Factors affecting the sensitivity and consistency of the Koelle-Friedenwald histochemical method for localization of acetylcholinesterase. Brain Res. Bull. 12: 543-553.
22. Wamsley JK, Palacios JM, Young WS III, Kuhar MJ (1981) Autoradiographic determination of neurotransmitter receptor distributions in the cerebral and cerebellar cortices. J. Histochem. Cytochem. 29: 125-135.
23. Young LJ, Winslow JT, Nilsen R, Insel TR (1997) Species differences in V1a receptor gene expression in monogamous and nonmonogamous voles: behavioral consequences. Behav Neurosci. 111: 599-605.
24. Young LJ, Lim MM, Gingrich B, Insel TR (2001) Cellular mechanisms of social attachment. Horm. Behav. 40: 133-138.
25. Young WS III, Kuhar MJ (1981) Neurotensin receptor localization by light microscopic autoradiography in rat brain. Brain Res. 206: 273-285.
26. Young WS III, Niehoff D, Kuhar MJ, Beer B, Lippa AS (1981) Multiple benzodiazepine receptor localization by light microscopic radiohistochemistry. J. Pharmacol. Exp. Ther. 216: 425-430.