Characterization of Recombinant CEL-I, a GalNAc-Specific C-Type Lectin, Expressed in Escherichia coli Using an Artificial Synthetic Gene

Journal of Biochemistry

Volumn 135, Issue 1, 2004, Pages 101-107

  Hatakeyama, Tomomitsu ^a   Shiba, Kouhei ^a   Matsuo, Noriaki ^a   Fujimoto, Tokiko ^a   Oda, Tatsuya ^a   Sugawara, Hajime ^b   Aoyagi, Haruhiko ^a  

^a NAGASAKI UNIVERSITY   (Japan)

^b RIKEN   (Japan)

Author keywords

Artificial gene; C type lectin; Crystal; Cucumaria echinata; Cytotoxicity

Indexed keywords

CALCIUM; CARBOHYDRATE; CEL 1 PROTEIN; LECTIN; MERCAPTOETHANOL; METHIONINE; OLIGONUCLEOTIDE; PROTEIN; RECOMBINANT CEL 1 PROTEIN; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG; UREA;

ARTICLE; BINDING AFFINITY; BINDING SITE; CELL INCLUSION; CIRCULAR DICHROISM; CONFORMATIONAL TRANSITION; CRYSTAL; CUCUMARIA ECHINATA; CYTOTOXICITY; ESCHERICHIA COLI; HEMAGGLUTINATION; HEMAGGLUTINATION INHIBITION TEST; HOLOTHUROIDEA; HUMAN; HUMAN CELL; INVERTEBRATE; NONHUMAN; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN STRUCTURE; ULTRAVIOLET SPECTROSCOPY;

ACETYLGALACTOSAMINE; AMINO ACID SEQUENCE; BASE SEQUENCE; CRYSTALLIZATION; DOSE-RESPONSE RELATIONSHIP, DRUG; ESCHERICHIA COLI; GENES, SYNTHETIC; HELA CELLS; HEMAGGLUTININS; HUMANS; LECTINS, C-TYPE; MOLECULAR SEQUENCE DATA; RECOMBINANT PROTEINS; X-RAY DIFFRACTION;

CUCUMARIA; CUCUMARIA ECHINATA; ESCHERICHIA COLI; HOLOTHUROIDEA; INVERTEBRATA; SYNTHETIC CONSTRUCT;

EID: 1642463060     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/jb/mvh012     Document Type: Article

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