Three-dimensional visualization analysis of in vitro cultured bone fabricated by rat marrow mesenchymal stem cells

Biochemical and Biophysical Research Communications

Volumn 316, Issue 3, 2004, Pages 943-948

  Kihara, Takanori ^a   Oshima, Akira ^a   Hirose, Motohiro ^a   Ohgushi, Hajime ^a  

^a NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY AIST   (Japan)

Author keywords

Bone mineralization; Calcein; Confocal laser scanning microscope; Mesenchymal stem cells; Mineralized nodule; Osteogenic differentiation; Three dimensional visualization

Indexed keywords

BIOLOGICAL MARKER; CALCEIN;

ANIMAL CELL; ANIMAL CELL CULTURE; ARTICLE; BONE DEVELOPMENT; BONE MATRIX; BONE MINERALIZATION; CELL SHAPE; COMPUTER ANALYSIS; CONFOCAL LASER MICROSCOPY; CONTROLLED STUDY; HEMATOPOIETIC STEM CELL; IN VITRO STUDY; MESENCHYME CELL; NONHUMAN; OSSIFICATION; OSTEOBLAST; PRIORITY JOURNAL; RAT; TISSUE ENGINEERING; TISSUE FIXATION;

ALKALINE PHOSPHATASE; ANIMALS; BONE AND BONES; BONE MARROW CELLS; CELL DIFFERENTIATION; CELLS, CULTURED; DNA; FLUORESCEINS; IMAGE PROCESSING, COMPUTER-ASSISTED; IMAGING, THREE-DIMENSIONAL; MESODERM; MICROSCOPY, CONFOCAL; RATS; STEM CELLS; TIME FACTORS;

EID: 1642408518     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbrc.2004.02.134     Document Type: Article

References (26)

1. Owen M., Friedenstein A.J. Stromal stem cells: marrow-derived osteogenic precursors. Ciba Found. Symp. 136:1988;42-60.
2. Galmiche M.C., Koteliansky V.E., Briere J., Herve P., Charbord P. Stromal cells from human long-term marrow cultures are mesenchymal cells that differentiate following a vascular smooth muscle differentiation pathway. Blood. 82:1993;66-76.
3. Bruder S.P., Fink D.J., Caplan A.I. Mesenchymal stem cells in bone development, bone repair, and skeletal regeneration therapy. J. Cell Biochem. 56:1994;283-294.
4. Pittenger M.F., Mackay A.M., Beck S.C., Jaiswal R.K., Douglas R., Mosca J.D., Moorman M.A., Simonetti D.W., Craig S., Marshak D.R. Multilineage potential of adult human mesenchymal stem cells. Science. 284:1999;143-147.
5. Jiang Y., Jahagirdar B.N., Reinhardt R.L., Schwartz R.E., Keene C.D., Ortiz-Gonzalez X.R., Reyes M., Lenvik T., Lund T., Blackstad M., Du J., Aldrich S., Lisberg A., Low W.C., Largaespada D.A., Verfaillie C.M. Pluripotency of mesenchymal stem cells derived from adult marrow. Nature. 418:2002;41-49.
6. Ohgushi H., Goldberg V.M., Caplan A.I. Heterotopic osteogenesis in porous ceramics induced by marrow cells. J. Orthop. Res. 7:1989;568-578.
7. Dohi Y., Ohgushi H., Tabata S., Yoshikawa T., Dohi K., Moriyama T. Osteogenesis associated with bone gla protein gene expression in diffusion chambers by bone marrow cells with demineralized bone matrix. J. Bone Miner. Res. 7:1992;1173-1180.
8. Ohgushi H., Caplan A.I. Stem cell technology and bioceramics: from cell to gene engineering. J. Biomed. Mater. Res. 48:1999;913-927.
9. Maniatopoulos C., Sodek J., Melcher A.H. Bone formation in vitro by stromal cells obtained from marrow of young adult rats. Cell Tissue Res. 254:1988;317-330.
10. Leboy P.S., Beresford J.N., Devlin C., Owen M.E. Dexamethasone induction of osteoblast mRNAs in rat marrow stromal cell cultures. J. Cell Physiol. 146:1991;370-378.
11. Davies J.E., Chernecky R., Lowenberg B., Shiga A. Deposition and resorption of calcified matrix in vitro by rat marrow cells. Cells Mater. 1:1991;3-15.
12. Ohgushi H., Dohi Y., Katuda T., Tamai S., Tabata S., Suwa Y. In vitro bone formation by rat marrow cell culture. J. Biomed. Mater. Res. 32:1996;333-340.
13. Service R.F. Tissue engineers build new bone. Science. 289:2000;1498-1500.
14. Ringe J., Kaps C., Burmester G.R., Sittinger M. Stem cells for regenerative medicine: advances in the engineering of tissues and organs. Naturwissenschaften. 89:2002;338-351.
15. Davies J.E., Ottensmeyer P., Shen X., Hashimoto M., Peel S.A.F. Early extracellular matrix synthesis by bone cells. Davies J.E. In the Bone-Biomaterial Interface. 1991;214-228 University of Toronto Press, Toronto.
16. Lowenberg B., Chernecky A., Shiga A., Davies J.E. Mineralized matrix production by osteoblasts on solid titanium in vitro by rat marrow cells. Cells Mater. 1:1991;177-187.
17. Simonetti O., Hoogstraate A.J., Bialik W., Kempenaar J.A., Schrijvers A.H., Bodde H.E., Ponec M. Visualization of diffusion pathways across the stratum corneum of native and in-vitro-reconstructed epidermis by confocal laser scanning microscopy. Arch. Dermatol. Res. 287:1995;465-473.
18. Andjelkovic A.V., Zochowski M.R., Morgan F., Pachter J.S. Qualitative and quantitative analysis of monocyte transendothelial migration by confocal microscopy and three-dimensional image reconstruction. In Vitro Cell Dev. Biol. Anim. 37:2001;111-120.
19. Uchimura E., Machida H., Kotobuki N., Kihara T., Kitamura S., Ikeuchi M., Hirose M., Miyake J., Ohgushi H. In-situ visualization and quantification of mineralization of cultured osteogenetic cells. Calcif. Tissue Int. 73:2003;575-583.
20. 2+ binding to the sarcoplasmic reticulum: use of a high-affinity fluorescent calcium indicator. Biophys. J. 18:1977;3-22.
21. Okumura M., Ohgushi H., Tamai S. Bonding osteogenesis in coralline hydroxyapatite combined with bone marrow cells. Biomaterials. 12:1991;411-416.
22. Yoshikawa T., Ohgushi H., Tamai S. Immediate bone forming capability of prefabricated osteogenic hydroxyapatite. J. Biomed. Mater. Res. 32:1996;481-492.
23. Reddi A.H., Sullivan N.E. Matrix-induced endochondral bone differentiation: influence of hypophysectomy, growth hormone, and thyroid-stimulating hormone. Endocrinology. 107:1980;1291-1299.
24. Lee L.L., Lee J.S., Waldman S.D., Casper R.F., Grynpas M.D. Polycyclic aromatic hydrocarbons present in cigarette smoke cause bone loss in an ovariectomized rat model. Bone. 30:2002;917-923.
25. Kamioka H., Honjo T., Takano-Yamamoto T. A three-dimensional distribution of osteocyte processes revealed by the combination of confocal laser scanning microscopy and differential interference contrast microscopy. Bone. 28:2001;145-149.
26. Cane V., Marotti G., Volpi G., Zaffe D., Palazzini S., Remaggi F., Muglia M.A. Size and density of osteocyte lacunae in different regions of long bones. Calcif. Tissue Int. 34:1982;558-563.