Pseudospecificity of the Acidic Patch of Plastocyanin for the Interaction with Cytochrome f

Journal of the American Chemical Society

Volumn 126, Issue 10, 2004, Pages 3028-3029

  Sato, Katsuko ^a   Kohzuma, Takamitsu ^b   Dennison, Christopher ^a  

^a NEWCASTLE UNIVERSITY   (United Kingdom)

^b IBARAKI UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOCHROME F; PLASTOCYANIN;

ACIDITY; ALGA; ARTICLE; DRYOPTERIDACEAE; ELECTRON TRANSPORT; IONIC STRENGTH; MOLECULAR INTERACTION; PROTEIN PROTEIN INTERACTION;

ALGAE, GREEN; CYTOCHROMES F; DRYOPTERIS; HYDROGEN-ION CONCENTRATION; KINETICS; MODELS, MOLECULAR; OSMOLAR CONCENTRATION; PLANT PROTEINS; PLASTOCYANIN; STRUCTURE-ACTIVITY RELATIONSHIP; SUBSTRATE SPECIFICITY;

EID: 1642390054     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja038188k     Document Type: Article

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19. -1 (Synechococcus).14
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22. Oxidation of cyt f(II) by the various PCu(II)s was monitored at 421 nm on an Applied Photophysics SX.18MV stopped-flow reaction analyzer at 25 °C with the proteins in 20 mM Tris pH 7.5. All rate constants were obtained under pseudo-first-order conditions [with PCu(II) in greater than 10-fold excess using cyt f(II) concentrations of typically 0.1 μM] and are an average of at least five determinations using the same solutions. In all cases, linear plots of first-order rate constants against [PCu(II)] were obtained. For the ionic strength dependence studies the appropriate amount of NaCl (BDH, AnalaR) was added to the buffer to give total ionic strengths in the range 1 = 0.016-2.576 M.
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