Cryopreserved and lyophilized cultured epidermal allografts in the treatment of leg ulcers: A pilot study

Journal of the European Academy of Dermatology and Venereology

Volumn 18, Issue 2, 2004, Pages 173-179

  Navratilova Z ^a   Slonkova V ^a   Semradova V ^a   Adler, J ^b  

^a ST ANNE S UNIVERSITY HOSPITAL   (Czech Republic)

^b UNIVERSITY HOSPITAL BRNO   (Czech Republic)

Author keywords

Cryopreservation; Keratinocytes; Leg ulcers; Lyophilization

Indexed keywords

ADULT; AGED; ALLOGRAFT; ANALGESIA; ARTICLE; CELL CULTURE; CLINICAL ARTICLE; CLINICAL TRIAL; CONTROLLED CLINICAL TRIAL; CONTROLLED STUDY; CRYOPRESERVATION; FEMALE; FREEZE DRYING; HUMAN; INTERMETHOD COMPARISON; KERATINOCYTE; LEG ULCER; MALE; PAIN ASSESSMENT; PRIORITY JOURNAL; ROOM TEMPERATURE; SKIN GRAFT; STATISTICAL ANALYSIS; STATISTICAL SIGNIFICANCE; SURGICAL TECHNIQUE; TREATMENT OUTCOME; ULCER HEALING;

ADULT; AGED; AGED, 80 AND OVER; CELL TRANSPLANTATION; CELLS, CULTURED; CHRONIC DISEASE; CRYOPRESERVATION; EPIDERMIS; FEMALE; FREEZE DRYING; HUMANS; MALE; MIDDLE AGED; PILOT PROJECTS; TRANSPLANTATION, HOMOLOGOUS; VARICOSE ULCER; WOUND HEALING;

EID: 1642373971     PISSN: 09269959     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1468-3083.2004.00873.x     Document Type: Article

References (41)

1. Teepe RGC, Koebrugge EJ, Ponec M, Vermeer BJ. Fresh versus cryopreserved cultured allografts for the treatment of chronic skin ulcers. Br J Dermatol 1990; 122: 81-89.
2. Rheinwald JG, Green H. Serial cultivation of strains of human epidermal keratinocytes: The formation of colonies from single cells. Cell 1975; 6: 331-344.
3. Green H, Kehinde O, Thomas J. Growth of cultured human epidermal cells into multiple epithelia suitable for grafting. Proc Natl Acad Sci USA 1979; 76: 5665-5668.
4. O'Connor NE, Mulliken JB, Banks-Schlegel S et al. Grafting of burns with cultured epithelium prepared from autologous epidermal cells. Lancet 1981; 1: 75-78.
5. Hefton JM, Madden MR, Finkelstein JL, Shires TG. Grafting of burn patients with allografts of cultured epidermal cells. Lancet 1983; 2: 428-430.
6. Donati L, Magliacani G, Bormioli M et al. Clinical experience with keratinocyte grafts. Burns 1992; 18S: 19-26.
7. Brychta P, Suchánek I, Rihová H et al. Cultured epidermal allografts for the treatment of deep dermal burns. Acta Chir Plast 1995; 37: 20-24.
8. Teepe RG, Kreis RW, Koebrugge EJ et al. The use of cultured autologous epidermis in the treatment of extensive burn wounds. J Trauma 1990; 30: 269-275.
9. Hefton JM, Caldwell D, Biozes DG et al. Grafting of skin ulcers with cultured autologous epidermal cells. J Am Acad Dermatol 1986; 14: 399-405.
10. Leigh IM, Purkis PE. Culture grafted leg ulcers. Clin Exp Dermatol 1986; 11: 650-652.
11. Leigh IM, Purkis PE, Navsaria HA, Phillips TJ. Treatment of chronic venous leg ulcers with sheets of cultured allogenic keratinocytes. Br J Dermatol 1987; 117: 591-597.
12. Carter DM, Lin AN, Varghese MC et al. Treatment of junctional epidermolysis bullosa with epidermal autografts. J Am Acad Dermatol 1987; 17: 246-250.
13. Gallico GG, O'Connor NE, Compton CC et al. Cultured epithelial autografts for giant congenital nevi. Plast Reconstr Surg 1989; 84: 1-9.
14. Phillips TJ, Gilchrest BA. Cultured allogeneic keratinocyte grafts in the management of wound healing: Prognostic factors. J Dermatol Surg Oncol 1989; 15: 1169-1176.
15. Slonková V, Kino-oka M, Mazza S et al. Cultured epithelial autografts for the management of a chronic pretibial leg ulcer due to congenital valvular aplasia. Dermatology 1999; 198: 101-103.
16. Eaglstein WH, Falanga V. Tissue engineering and the development of Apligraf, a human skin equivalent. Clin Ther 1997; 19: 894-905.
17. Beele H, Naeyaert JM, Goeteyn M et al. Repeated cultured epidermal allografts in the treatment of chronic leg ulcers of various origin. Dermatologica 1991; 183: 31-35.
18. Phillips TJ, Kehinde O, Green H, Gilchrest BA. Treatment of skin ulcers with cultured epidermal allografts. J Am Acad Dermatol 1989; 21: 191-199.
19. Jones JE, Nelson EA. Skin grafting for venous leg ulcers. Cochrane Database Syst Rev 2000; 2: CD001737.
20. Phillips TJ, Gilchrest BA. Cultured epidermal grafts in the treatment of leg ulcers. Adv Dermatol 1990; 5: 33-50.
21. Terskikh W, Vasiliev AV. Cultivation and transplantation of epidermal keratinocytes. Int Rev Cytol 1999; 188: 41-72.
22. Thivolet J, Faure M, Demidem A. Long term survival and immunological tolerance of human epidermal allografts produced in culture. Transplantation 1986; 4: 274-280.
23. Phillips TJ, Leigh IM, Purkis PE. Expression of keratin polypeptides and histocompatibility antigens in wounds treated with allografts of cultured keratinocytes. J Invest Dermatol 1986; 87: 101.
24. Eisenger M. Regeneration of epidermis by cells grown in tissue culture. J Am Acad Dermatol 1985; 12: 402-408.
25. Aubock J, Irschick E, Romani A. Rejection, after slightly prolonged survival time, of Langerhans cell-free allogeneic cultured epidermis used for wound coverage in humans. Transplantation 1988; 45: 730-737.
26. Brain A, Purkis P, Coates P et al. Survival of cultured allogeneic keratinocytes transplanted to dermal bed assessed with probe specific for Y chromosome. Br Med J 1989; 208: 917-919.
27. Burt AM, Pallett CD, Sloane JP. Survival of cultured allografts in patients with burns assessed with probe specific for Y chromosome. Br Med J 1989; 298: 915-917.
28. De Luca M, Albanese E, Cancedda R et al. Treatment of leg ulcers with cryopreserved allogeneic cultured epithelium. Arch Dermatol 1992; 128: 633-638.
29. Duinslaeger L, Verbeken G, Reper P et al. Lyophilized keratinocyte cell lysates contain multiple mitogenic activities and stimulate closure of meshed skin autograft-covered burn wounds with efficiency similar to that of fresh allogeneic keratinocyte cultures. Plast Reconstr Surg 1996; 98: 110.
30. McKay IA, Leigh IM. Epidermal cytokines and their roles in cutaneous wound healing. Br J Dermatol 1991; 124: 513-518.
31. Resl V. Hojení chronických ran. Grada Publishing, 1997; 201: 177-179, 326-328.
32. Adler J, Komárková J, Brychta P. Transplantation von Keratinocyten und Wundheilung. Wundheilung und Wundauflagen. Wissenschaftliche Verlagsgesellschaft, Stuttgart, 1996.
33. Límoví M, Grekin RC. Synthetic membranes and cultured keratinocyte grafts. J Am Acad Dermatol 1990; 23: 713-719.
34. Marcusson JA, Lindgren C, Berghard A, Toftgard R. Allogeneic cultured keratinocytes in the treatment of leg ulcers (a pilot study). Acta Derm Venereol 1992; 72: 61-64.
35. Teepe RG, Koebrugge EJ, Zeeman RJ et al. The treatment of chronic skin ulcers with cryo-preserved cultured allogeneic epidermis. Ned Tijdschr Geneeskd 1990; 134: 750-754.
36. Somers T, Duinslaeger L, Delaey B et al. Stimulation of epithelial healing in chronic postoperative otorrhea using lyophilized cultured keratinocyte lysates. Am J Otol 1997; 18: 702-706.
37. Bolivar-Flores YJ, Kuri-Harcuch W. Frozen allogeneic human epidermal cultured sheets for the cure of complicated leg ulcers. Dermatol Surg 1999; 25: 610-617.
38. Lindgren C, Marcusson JA, Toftgard R. Treatment of venous leg ulcers with cryopreserved cultured allogeneic keratinocytes: A prospective open controlled study. Br J Dermatol 1998; 139: 271-275.
39. Harvima IT, Virnes S, Kauppinen L et al. Cultured allogeneic skin cells are effective in the treatment of chronic diabetic leg and foot ulcers. Acta Derm Venereol 1999; 79: 217-220.
40. Pospíšilová A. Lécba ulcerací dolních koncetin kultivovanými epidermálními štepy. Prakt Flebol 1995; 1: 8-11.
41. Hunziker T, Limat A. Cultured keratinocyte grafts. Curr Probl Dermatol 1999; 27: 57-64.