Cascade pathway of filopodia formation downstream of SCAR

Journal of Cell Science

Volumn 117, Issue 6, 2004, Pages 837-848

  Biyasheva, Assel ^a   Svitkina, Tatyana ^a   Kunda, Patricia ^b   Baum, Buzz ^b   Borisy, Gary ^a  

^a NORTHWESTERN UNIVERSITY   (United States)

^b UNIVERSITY COLLEGE LONDON   (United Kingdom)

Author keywords

Actin; Drosophila cell lines; Filopodia; Lamellipodia; LCM; SCAR; WASp

Indexed keywords

ACTIN; DROSOPHILA PROTEIN; PROTEIN SCAR; PROTEIN WASP; RNA; UNCLASSIFIED DRUG;

ARTICLE; CELL CULTURE; CELL KINETICS; CELL ORGANELLE; CELL SPREADING; CELL TYPE; CONTROLLED STUDY; DROSOPHILA; ELECTRON MICROSCOPY; FILOPODIUM; LAMELLIPODIUM; LASER CAPTURE MICRODISSECTION; MORPHOMETRICS; NONHUMAN; NUCLEOTIDE SEQUENCE; PHENOTYPE; PRIORITY JOURNAL; PROTEIN DEPLETION; PROTEIN FAMILY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SIGNAL TRANSDUCTION; WESTERN BLOTTING;

ANIMALS; CELL LINE; DROSOPHILA; DROSOPHILA PROTEINS; GTP-BINDING PROTEINS; MICROFILAMENT PROTEINS; PSEUDOPODIA; RAC GTP-BINDING PROTEINS; RNA INTERFERENCE; SIGNAL TRANSDUCTION; WISKOTT-ALDRICH SYNDROME PROTEIN;

HEXAPODA; VESPIDAE;

EID: 1642365541     PISSN: 00219533     EISSN: None     Source Type: Journal    
DOI: 10.1242/jcs.00921     Document Type: Article

References (46)

1. Bear, J. E., Rawls, J. F. and Saxe, C. L., III (1998). SCAR, a WASP-related protein, isolated as a suppressor of receptor defects in late Dictyostelium development. J. Cell Biol. 142, 1325-1335.
2. Bear, J. E., Svitkina, T. M., Krause, M., Schafer, D. A., Loureiro, J. J., Strasser, G. A., Maly, I. V., Chaga, O. Y., Cooper, J. A., Borisy, G. G. et al. (2002). Antagonism between Ena/VASP proteins and actin filament capping regulates fibroblast motility. Cell 109, 509-521.
3. Ben-Yaacov, S., le Borgne, R., Abramson, I., Schweisguth, F. and Schejter, E. D. (2001). Wasp, the Drosophila Wiskott-Aldrich syndrome gene homologue, is required for cell fate decisions mediated by Notch signaling. J. Cell Biol. 152, 1-13.
4. Borisy, G. G. and Svitkina, T. M. (2000). Actin machinery: pushing the envelope. Curr. Opin. Cell Biol. 12, 104-112.
5. Clemens, J. C., Worby, C. A., Simonson-Leff, N., Muda, M., Maehama, T., Hemmings, B. A. and Dixon, J. E. (2000). Use of double-stranded RNA interference in Drosophila cell lines to dissect signal transduction pathways. Proc. Natl. Acad. Sci. USA 97, 6499-6503.
6. Eden, S., Rohatgi, R., Podtelejnikov, A. V., Mann, M. and Kirschner, M. W. (2002). Mechanism of regulation of WAVE1-induced actin nucleation by Rac1 and Nck. Nature 418, 790-793.
7. Falet, H., Hoffmeister, K. M., Neujahr, R. and Hartwig, J. (2002). Normal Arp2/3 complex activation in platelets lacking WASp. Blood 100, 2113-2122.
8. Hahne, P., Sechi, A., Benesch, S. and Small, J. V. (2001). Scar/WAVE is localised at the tips of protruding lamellipodia in living cells. FEBS Lett. 492, 215-220.
9. Hakeda-Suzuki, S., Ng, J., Tzu, J., Dietzl, G., Sun, Y., Harms, M., Nardine, T., Luo, L. and Dickson, B. J. (2002). Rac function and regulation during Drosophila development. Nature 416, 438-442.
10. Hall, A. (1998). Rho GTPases and the actin cytoskeleton. Science 279, 509-514.
11. Higgs, H. N. and Pollard, T. D. (2000). Activation by Cdc42 and PIP(2) of Wiskott-Aldrich syndrome protein (WASp) stimulates actin nucleation by Arp2/3 complex. J. Cell Biol. 150, 1311-1320.
12. Higgs, H. N. and Pollard, T. D. (2001). Regulation of actin filament network formation through ARP2/3 complex: activation by a diverse array of proteins. Annu. Rev. Biochem. 70, 649-676.
13. Hudson, A. M. and Cooley L. (2002). A subset of dynamic actin rearrangements in Drosophila requires the Arp2/3 complex. J. Cell Biol. 156, 677-687.
14. Krugmann, S., Jordens, I., Gevaert, K., Driessens, M., Vandekerckhove, J. and Hall, A. (2001). Cdc42 induces filopodia by promoting the formation of an IRSp53:Mena complex. Curr. Biol. 11, 1645-1655.
15. Kunda, P., Craig, G., Dominguez, V. and Baum, B. (2003). Abi, Sra1, and Kette control the stability and localization of SCAR/WAVE to regulate the formation of actin-based protrusions. Curr. Biol. 13, 1867-1875.
16. Kureishy, N., Sapountzi, V., Prag, S., Anilkumar, N. and Adams, J. C. (2002). Fascins, and their roles in cell structure and function. Bioessays 24, 350-361.
17. Lanier, L. M., Gates, M. A., Witke, W., Menzies, A. S., Wehman, A. M., Macklis, J. D., Kwiatkowski, D., Soriano, P. and Gertler, F. B. (1999). Mena is required for neurulation and commissure formation. Neuron 22, 313-325.
18. Linder, S., Higgs, H., Hufner, K., Schwarz, K., Pannicke, U. and Aepfelbacher, M. (2000). The polarization defect of Wiskott-Aldrich syndrome macrophages is linked to dislocalization of the Arp2/3 complex. J. Immunol. 165, 221-225.
19. Lommel, S., Benesch, S., Rottner, K., Franz, K. T., Wehland, J. and Kuhn, R. (2001). Actin pedestal formation by enteropathogenic Escherichia coli and intracellular motility of Shigella flexneri are abolished in N-WASP-defective cells. EMBO Rep. 2, 850-857.
20. Lyubimova, A., Bershadsky, A. D. and Ben-Ze'ev, A. (1997). Autoregulation of actin synthesis responds to monomeric actin levels. J. Cell Biochem. 65, 469-478.
21. Machesky, L. M. and Insall, R. H. (1998). Scar1 and the related Wiskott-Aldrich syndrome protein, WASP, regulate the actin cytoskeleton through the Arp2/3 complex. Curr. Biol. 8, 1347-1356.
22. Mallavarapu, A. and Mitchison, T. (1999). Regulated actin cytoskeleton assembly at filopodium tips controls their extension and retraction. J. Cell Biol. 146, 1097-1106.
23. Miki, H., Yamaguchi, H., Suetsugu, S. and Takenawa, T. (2000). IRSp53 is an essential intermediate between Rac and WAVE in the regulation of membrane ruffling. Nature 408, 732-735.
24. Mullins, R. D., Heuser, J. A. and Pollard, T. D. (1998). The interaction of Arp2/3 complex with actin: nucleation, high affinity pointed end capping, and formation of branching networks of filaments. Proc. Natl. Acad. Sci. USA 95, 6181-6816.
25. Nozumi, M., Nakagawa, H., Miki, H., Takenawa, T. and Miyamoto, S. (2003). Differential localization of WAVE isoforms in filopodia and lamellipodia of the neuronal growth cone. J. Cell Sci. 116, 239-246.
26. Peng. J., Wallar, B. J., Flanders, A., Swiatek, P. J. and Alberts, A. S. (2003). Disruption of the Diaphanous-related formin Drf1 gene encoding mDia1 reveals a role for Drf3 as an effector for Cdc42. Curr. Biol. 13, 534-545.
27. Qualmann, B., Kessels, M. M., Roos, J., DiGregorio, P. J. and Kelly, R. B. (2002). Endocytosis and the cytoskeleton. Int. Rev. Cytol. 220, 93-144.
28. Rogers, S. L., Wiedemann, U., Stuurman, N. and Vale, R. D. (2003). Molecular requirements for actin-based lamella formation in Drosophila S2 cells. J. Cell Biol. 162, 1079-1088.
29. Rohatgi, R., Ho, H. Y. and Kirschner, M. W. (2000). Mechanism of N-WASP activation by CDC42 and phosphatidylinositol 4,5-bisphosphate. J. Cell Biol. 150, 1299-1310.
30. Rottner, K., Behrendt, B., Small, J. V. and Wehland, J. (1999). VASP dynamics during lamellipodia protrusion. Nat. Cell Biol. 1, 321-322.
31. Schafer, D. A. (2002). Coupling actin dynamics and membrane dynamics during endocytosis. Curr. Opin. Cell Biol. 14, 76-81.
32. Small, J. V., Stradal, T., Vignal, E. and Rottner, K. (2002). The lamellipodium: where motility begins. Trends. Cell Biol. 12, 112-120.
33. Snapper, S. B., Takeshima, F., Anton, I., Liu, C. H., Thomas, S. M., Nguyen, D., Dudley, D., Fraser, H., Purich, D., Lopez-Ilasaca, M. et al. (2001). N-WASP deficiency reveals distinct pathways for cell surface projections and microbial actin-based motility. Nat. Cell Biol. 3, 897-904.
34. Svitkina, T. M. and Borisy, G. G. (1998). Correlative light and electron microscopy of the cytoskeleton of cultured cells. Methods Enzymol. 298, 570-592.
35. Svitkina, T. M. and Borisy, G. G. (1999a). Progress in protrusion: the tell-tale scar. Trends Biochem. Sci. 24, 432-436.
36. Svitkina, T. M. and Borisy, G. G. (1999b). Arp2/3 complex and actin depolymerizing factor/cofilin in dendritic organization and treadmilling of actin filament array in lamellipodia. J. Cell Biol. 145, 1009-1026.
37. Svitkina, T. M., Verkhovsky, A. B., McQuade, K. M. and Borisy, G. G. (1997). Analysis of the actin-myosin II system in fish epidermal keratocytes: mechanism of cell body translocation. J. Cell Biol. 139, 397-415.
38. Svitkina, T. M., Bulanova, E. A., Chaga, O. Y., Vignjevic, D. M., Kojima, S., Vasiliev, J. M. and Borisy, G. G. (2003). Mechanism of filopodia initiation by reorganization of a dendritic network. J Cell Biol. 160, 409-421.
39. Takenawa, T. and Miki, H. (2001). WASP and WAVE family proteins: key molecules for rapid rearrangement of cortical actin filaments and cell movement. J. Cell Sci. 114, 1801-1809.
40. Tal, T., Vaizel-Ohayon, D. and Schejter, E. D. (2002). Conserved interactions with cytoskeletal but not signaling elements are an essential aspect of Drosophila WASp function. Dev. Biol. 243, 260-271.
41. Thrasher, A. J. (2002). Wasp in immune-system organization and function. Nat. Rev. Immunol. 2, 635-646.
42. Ui, K., Nishihara, S., Sakuma, M., Togashi, S., Ueda, R., Miyata, Y. and Miyake,T. (1994). Newly established cell lines from Drosophila larval CNS express neural specific characteristics. In Vitro Cell Dev. Biol. Anim. 30A, 209-216.
43. Vignjevic, D., Yarar, D., Welch, M. D., Peloquin, J., Svitkina, T. and Borisy, G. G. (2003). Formation of filopodia-like bundles in vitro from a dendritic network. J. Cell Biol. 160, 951-962.
44. Wood, W. and Martin, P. (2002). Structures in focus-filopodia. Int. J. Biochem. Cell Biol. 34, 726-730.
45. Yanagawa, S., Lee, J. S. and Ishimoto, A. (1998). Identification and characterization of a novel line of Drosophila Schneider S2 cells that respond to wingless signaling. J. Biol. Chem. 273, 32353-32359.
46. Zallen, J. A., Cohen, Y., Hudson, A. M., Cooley, L., Wieschaus, E. and Schejter, E. D. (2002). SCAR is a primary regulator of Arp2/3-dependent morphological events in Drosophila. J. Cell Biol. 156, 689-701.