Site-Directed Mutagenesis of the Conserved Threonine, Tryptophan, and Lysine Residues in the Starch-Binding Domain of Bacillus sp. Strain TS-23 α-Amylase

Current Microbiology

Volumn 48, Issue 4, 2004, Pages 280-284

  Lo, Huei Fen ^a   Chiang, Wen Ying ^a   Chi, Meng Chun ^b   Hu, Hui Yu ^a   Lin, Long Liu ^b  

^a HUNGKUANG UNIVERSITY   (Taiwan)

^b NATIONAL CHIAYI UNIVERSITY   (Taiwan)

Author keywords

[No Author keywords available]

Indexed keywords

AMYLASE; BACTERIAL ENZYME; ISOLEUCINE; LEUCINE; LYSINE; MUTANT PROTEIN; NICKEL COMPLEX; STARCH; THREONINE; TRYPTOPHAN;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; BACILLUS; BACTERIAL STRAIN; BINDING ASSAY; BINDING SITE; CARBOXY TERMINAL SEQUENCE; CHROMATOGRAPHY; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME BINDING; ENZYME PURIFICATION; ENZYME STRUCTURE; GENE MUTATION; GENETIC CONSERVATION; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN EXPRESSION; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS; SITE DIRECTED MUTAGENESIS; STRUCTURE ACTIVITY RELATION; STRUCTURE ANALYSIS;

ALPHA-AMYLASE; AMINO ACID SEQUENCE; BINDING SITES; CONSERVED SEQUENCE; LYSINE; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; SEQUENCE ALIGNMENT; STARCH; THREONINE; TRYPTOPHAN;

BACILLUS SP.; BACILLUS SP. TS-23; BACTERIA (MICROORGANISMS); POSIBACTERIA;

EID: 1642352838     PISSN: 03438651     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00284-003-4198-y     Document Type: Article

References (25)

1. Belshaw NJ, Williamson G (1990) Production and purification of a granular-starch-binding domain of glucoamylase 1 from Aspergillus niger. FEBS Lett 269:350-353
2. Boel E, Hjort I, Svensson B, Norris F, Norris KE, Fiil NP (1984) Glucoamylases G1 and G2 from Aspergillus niger are synthesized from two different but closely related mRNAs. EMBO J 3:1097-1102
3. Bradford MM (1976) A rapid and sensitive method for quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248-254
4. Clarke AJ, Svensson B (1984) The role of tryptophanyl residues in the function of Aspergillus niger glucoamylase G1 and G2. Carsberg Res Commun 49:111-122
5. Dalmia B, Schütte K, Nikolov Z (1995) Domain E of Bacillus macerans cyclodextrin glucanotransferase: An independent starch-binding domain. Biotechnol Bioeng 47:575-584
6. Din N, Forsythe IJ, Burtnick LD, Gilkes NR, Miller RC Jr, Warren RAJ, Kilburn DG (1994) The cellulose-binding domain of endoglucanase A (CenA) from Cellulomonas fimi: Evidence for the involvement of tryptophan residues in binding. Mol Microbiol 11:747-755
7. Goto M, Semimaru T, Furukawa K, Hayashida S (1994) Analysis of the raw starch-binding domain by mutation of a glucoamylase from Aspergillus awamori var. kawachi expressed in Saccharomyces cerevisiae. Appl Environ Microbiol 60:3926-3930
8. Janecěk Š, Ševčĺk J (1999) The evolution of starch-binding domain. FEBS Lett 456:119-125
9. Kadziola A, Abe J, Svensson B, Haser R (1994) Crystal and molecular structure of barley α-amylase. J Mol Biol 217:737-750
10. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685
11. Lawson CL, van Montfort R, Strokopytov B, Rozeboom HJ, Kalk KH, de Vries G, Penninga D, Dijikhuizen L, Dijkstra BW (1994) Nucleotide sequence and X-ray structure of cyclodextrin glycosyl-transferase from Bacillus circulans strain 251 in a maltose-dependent crystal form. J Mol Biol 236:590-600
12. Lin LL, Hsu WH, Chu WS (1997) A gene encoding for an α-amylase from thermophilic Bacillus sp. strain TS-23 and its expression in Escherichia coli. J Appl Microbiol 82:325-334
13. Lin LL, Lo HF, Chi MC, Ku KL (2003) Functional expression of the raw starch-binding domain of Bacillus sp. strain TS-23 α-amylase in recombinant Escherichia coli. Starch/Stärke 55:197-202
14. Lo HF, Lin LL, Li CC, Hsu WH, Chang CT (2001) The N-terminal signal sequence and the last 98 amino acids are not essential for the secretion of Bacillus sp. TS-23 α-amylase. Curr Microbiol 43:170-175
15. Lo HF, Lin LL, Chiang WY, Chi MC, Hsu WH, Chang CT (2002) Deletion analysis of the C-terminal region of the α-amylase of Bacillus sp. strain TS-23. Arch Microbiol 178:115-123
16. Machius M, Wiegand G, Huber R (1995) Crystal structure of calcium-depleted Bacillus licheniformis α-amylase at 2.2Å resolution. J Mol Biol 267:661-672
17. Matsuura Y, Kusunoki M, Harada W, Kakudo M (1984) Structure and possible catalytic residues for Taka-amylase. J Biochem (Tokyo) 95:697-702
18. Mikami B, Adachi M, Kage T, Sarikaya E, Nanmori T, Shinke R, Utsumi S (1999) Structure of raw starch-digesting Bacillus cereus β-amylase complexed with maltose. Biochemistry 38:7050-7061
19. Nanmori T, Nagai M, Shimizu Y, Shinke R, Mikami B (1993) Cloning of the β-amylase gene from Bacillus cereus and characteristics of the primary structure of the enzyme. Appl Environ Microbiol 59:623-627
20. Ramasubbu N, Paloth V, Luo Y, Brayer GD, Levine M (1996) Structure of human salivary α-amylase at 1.6 Å resolution: Implication for its role in the oral cavity. Acta Crystallogr 52:435-446
21. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: A laboratory manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press, pp. 17.2-17.44
22. Sigurskjold BW, Svensson B, Williamson G, Driguez H (1994) Thermodynamics of ligand binding to the starch-binding domain of glucoamylase from Aspergillus niger. Eur J Biochem 225:133-141
23. Sorimachi K, Jacks AJ, Gal-Coëffet MF, Williamson G, Archer DB, Williamson MP (1996) Solution structure of the granular starch-binding domain of glucoamylase from Aspergillus niger by nuclear magnetic resonance spectroscopy. J Mol Biol 259:970-987
24. Strokopytov B, Knegtel RM, Penninga D, Rozeboom HJ, Kalk KH, Dijkhuizen L, Dijkstra BW (1996) Structure of cyclodextrin glycosyltransferase complexed with a maltononaose inhibitor at 2.6 Å resolution: Implications for product specificity. Biochemistry 35:4241-4249
25. Williamson MP, Le Gal-Coëffet MF, Sorimachi K, Furmiss CSM, Archer DB, Williamson G (1997) Function of conserved tryptophans in the Aspergillus niger glucoamylase 1 starch binding domain. Biochemistry 36:7535-7539