Both lysine-clusters of the NH2-terminal prion-protein fragment PrP23-110 are essential for t-PA mediated plasminogen activation

Thrombosis and Haemostasis

Volumn 91, Issue 3, 2004, Pages 465-472

  Epple, Guido ^a   Langfeld, Kristina ^a   Baier, Michael ^b   Holzhütter, Hermann Georg ^a   Schleuning, Wolf Dieter ^c,d   Köttgen, Eckart ^a   Geßner, Reinhard ^a   Praus, Michael ^a  

^a HUMBOLDT UNIVERSITY   (Germany)

^b ROBERT KOCH INSTITUTE   (Germany)

^c BAYER PHARMA AG   (Germany)

^d Paion GmbH   (Germany)

Author keywords

Heparins glycosaminoglycans; Plasminogen activators; Protein function activity; Proteolysis pericellular

Indexed keywords

ALANINE; GLYCOSAMINOGLYCAN; HEPARIN; LYSINE; MUTANT PROTEIN; PLASMINOGEN; PRION PROTEIN; TISSUE PLASMINOGEN ACTIVATOR; PLASMIN; PLASMINOGEN ACTIVATOR; RECOMBINANT PROTEIN;

AMINO TERMINAL SEQUENCE; ARTICLE; BINDING KINETICS; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; HUMAN; MOLECULAR MECHANICS; OLIGOMERIZATION; PLASMINOGEN ACTIVATION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN FUNCTION; PROTEIN INTERACTION; PROTEIN MODIFICATION; SURFACE PLASMON RESONANCE; CHEMISTRY; DOSE RESPONSE; GENETICS; KINETICS; METABOLISM; MUTATION; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRION; PROTEIN TERTIARY STRUCTURE; TIME; WESTERN BLOTTING;

BLOTTING, WESTERN; DOSE-RESPONSE RELATIONSHIP, DRUG; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GLYCOSAMINOGLYCANS; HEPARIN; HUMANS; KINETICS; LYSINE; MUTATION; PLASMIN; PLASMINOGEN ACTIVATORS; PRIONS; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; SURFACE PLASMON RESONANCE; TIME FACTORS; TISSUE PLASMINOGEN ACTIVATOR;

EID: 1642299772     PISSN: 03406245     EISSN: None     Source Type: Journal    
DOI: 10.1160/th03-06-0382     Document Type: Article

References (27)

1. Ellis V, Daniels M, Misra R, et al.. Plasminogen activation is stimulated by prion protein and regulated in a copper-dependent manner. Biochemistry 2002; 41(22): 6891-6.
2. Praus M, Kettelgerdes G, Baier M, et al.. Stimulation of plasminogen activation by recombinant cellular prion protein is conserved in the NH(2)-terminal fragment PrP23-110. Thromb Haemost 2003; 89(5): 812-9.
3. Chen SG, Teplow DB, Parchi P, et al.. Truncated Forms of the Human Prion Protein in Normal Brain and in Prion Diseases. J Biol Chem 1995; 270(32): 19173-80.
4. Vincent B, Paitel E, Saftig P, et al.. The Disintegrins ADAM10 and TACE Contribute to the Constitutive and Phorbol Ester-regulated Normal Cleavage of the Cellular Prion Protein. J Biol Chem 2001; 276(41): 37743-6.
5. Madani R, Hulo S, Toni N, et al.. Enhanced hippocampal long-term potentiation and learning by increased neuronal expression of tissue-type plasminogen activator in transgenic: Mice. Embo J 1999; 18(11): 3007-12.
6. Hayden SM, Seeds NW. Modulated expression of plasminogen activator system components in cultured cells from dissociated mouse dorsal root ganglia. J Neurosci 1996; 16(7): 2307-17.
7. Chen ZL, Strickland S. Neuronal death in the hippocampus is promoted by plasmin-catalyzed degradation of laminin. Cell 1997; 91(7): 917-25.
8. Seeds NW, Williams BL, Bickford PC. Tissue plasminogen activator induction in Purkinje neurons after cerebellar motor learning. Science 1995; 270(5244): 1992-4.
9. Muller CM, Griesinger CB. Tissue plasminogen activator mediates reverse occlusion plasticity in visual cortex. Nat Neurosci 1998; 1(1): 47-53.
10. Wiman B, Collen D. Molecular mechanism of physiological fibrinolysis. Nature 1978; 272(5653): 549-50.
11. Bringmann P, Gruber D, Liese A, et al.. Structural features mediating fibrin selectivity of vampire bat plasminogen activators. J Biol Chem 1995; 270(43): 25596-603.
12. van Zonneveld AJ, Veerman H, Pannekoek H. On the interaction of the finger and the kringle-2 domain of fissue-type plasminogen activator with fibrin. Inhibition of kringle-2 binding to fibrin by epsilon-amino caproic acid. J Biol Chem 1986; 261(30): 14214-8.
13. Bok RA, Mangel WF. Quantitative characterization of the binding of plasminogen to intact fibrin clots, lysine-sepharose, and fibrin cleaved by plasmin. Biochemistry 1985; 24(13): 3279-86.
14. Ryon C, Prusiner SB, Legname G. Cooperative binding of dominant-negative prion protein to kringle domains. J Mol Biol 2003; 329(2): 323-33.
15. Warner RG, Hundt C, Weiss S, et al.. Identification of the heparan sulfate binding sites in the cellular prion protein. J Biol Chem 2002; 277(21): 18421-30.
16. Kovalchuk O, Tzaban S, Tal Y, et al.. Cellular heparan sulfate participates in the metabolism of prions. J Biol Chem 2003;18: 18.
17. Ben-Zaken O, Tzaban S, Tal Y, et al.. Cellular heparan sulfate participates in the metabolism of prions. J Biol Chem 2003;278(41):40041-9.
18. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227(259): 680-5.
19. Schagger H, von Jagow G. Blue native electrophoresis for isolation of membrane protein complexes in enzymatically active form. Anal Biochem 1991; 199(2): 223-31.
20. Brimacombe DB, Bennett AD, Wusteman FS, et al.. Characterization and polyanion-binding properties of purified recombinant prion protein. Biochem J 1999; 342(Pt 3): 605-13.
21. Andrade-Gordon P, Strickland S. Anticoagulant low molecular weight heparin does not enhance the activation of plasminogen by tissue plasminogen activator. J Biol Chem 1989; 264(26): 15177-81.
22. Medved L, Nieuwenhuizen W. Molecular mechanisms of initiation of fibrinolysis by fibrin. Thromb Haemost 2003; 89(3): 409-19.
23. Fischer MB, Roeckl C, Parizek P, et al.. Binding of disease-associated prion protein to plasminogen. Nature 2000; 408(6811): 479-83.
24. Allen RA. An enhancing effect of poly-lysine on the activation of plasminogen. Thromb Haemost 1982; 47(1): 41-5.
25. Zahn R. The Octapeptide Repeats in Mammalian Prion Protein Constitute a pH-dependent Folding and Aggregation Site. J Mol Biol 2003; 334(3): 477-88.
26. Caughey B, Raymond GJ. Sulfated polyanion inhibition of scrapie-associated PrP accumulation in cultured cells. J Virol 1993; 67(2): 643-50.
27. Mayor S. Small improvement seen in teenager with vCJD. BMJ 2003; 327(7418): 765.