Design and Synthesis of Visible Isotope-Coded Affinity Tags for the Absolute Quantification of Specific Proteins in Complex Mixtures

Bioconjugate Chemistry

Volumn 15, Issue 2, 2004, Pages 380-388

  Bottari, Patricia ^a   Aebersold, Ruedi ^c   Turecek, Frantisek ^a   Gelb, Michael H ^a,b  

^a UNIVERSITY OF WASHINGTON   (United States)

^b UNIVERSITY OF WASHINGTON SCHOOL OF MEDICINE   (United States)

^c INSTITUTE FOR SYSTEMS BIOLOGY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; BIOSYNTHESIS; CARBON; ISOTOPES; MASS SPECTROMETRY; MIXTURES;

ABSOLUTE QUANTIFICATION; BIOLOGICAL SAMPLES; CARBON-13; COMPLEX MIXTURE; IDENTIFICATION OF PROTEINS; ISOTOPE-CODED AFFINITY TAG; MASS SPECTROMETRIC ANALYSIS; NITROGEN-15; QUANTITATIVE DATA; RELATIVE QUANTIFICATION;

PEPTIDES;

CARBON 13; CYSTEINE; DEUTERIUM; IODOACETAMIDE; ISOTOPE; NITROGEN 15; REAGENT;

ARTICLE; ATOM; CELL LYSATE; CHEMICAL REACTION; COMPLEX FORMATION; ELECTROPHORESIS; MASS SPECTROMETRY; PROTEIN ANALYSIS; PROTEIN MODIFICATION; PROTEIN SYNTHESIS; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY;

EID: 1642281957     PISSN: 10431802     EISSN: None     Source Type: Journal    
DOI: 10.1021/bc034174s     Document Type: Article

References (13)

1. Gygi, S. P., and Aebersold, R. (2000) Mass spectrometry and proteomics. Curr. Opin. Chem. Biol. 4, 489-494.
2. Mo, W., and Karger, B. L. (2002) Analytical aspects of mass spectrometry and proteomics. Curr. Opin. Chem. Biol. 6, 666-675.
3. Turecek, F. (2002) Mass spectrometry in coupling with affinity capture-release and isotope-coded affinity tags for quantitative protein analysis. J. Mass Spectrom. 37, 1-14.
4. Gygi, S. P., Rist, B., Gerber, S. A., Turecek, F., Gelb, M. H., and Aebersold, R. (1999) Quantitative analysis of complex protein mixtures using isotope-coded affinity tags. Nat. Biotechnol. 17, 994-999.
5. Aebersold, R., and Mann, M. (2003) Mass spectrometry-based proteomics. Nature 422, 198-207.
6. Liu, H., Lin, D., and Yates, J. R. r. (2002) Multidimensional separations for protein/peptide analysis in the post-genomic era. Biotechniques 32, 898-902.
7. Shen, M., Guo, L., Wallace, A., Fitzner, J., Eisenman, J., Jacobson, E., and Johnson, R. S. (2003) Isolation and Isotope Labeling of Cysteine- and Methionine-containing Tryptic Peptides: Application to the Study of Cell Surface Proteolysis. Mol. Cell Proteomics 2, 315-324.
8. Lu, Y., Bottari, P., Turecek, F., Aebersold, R., and Gelb, M. H. (2003) Visible Isotope-Coded Affinity Tags for the Absolute Quantification of Specific Proteins in Complex Mixtures. Anal. Chem., submitted.
9. Gerber, S. A., Rush, J., Stemman, O., Kirschner, M. W., and Gygi, S. P. (2003) Absolute quantification of proteins and phosphoproteins from cell lysates by tandem MS. Proc. Natl. Acad. Sci. U.S.A. 100, 6940-6945.
10. Gamper, H. B., Reed, M. W., Cox, T., Virosco, J. S., Adams, A. D., Gall, A. A., Scholler, J. K., and Meyer, R. B. (1993) Facile preparation of nuclease resistant 3′ modified oligodeoxynucleotides. Nucleic Acids Res. 21, 145-150.
11. Doppler, T., Schmid, H., and Jurgen, H. (1979) On the Photochemistry of 2,1-Benzisoxazoles (Anthraniles) and on the Thermal and Photochemical Decomposition of 2-Azidoacylbenzenes in Strong Acidic Solution. Helv. Chim. Acta 62, 271.
12. Senter, P. D., Tansey, M. J., Lambert, J. M., and Blattler, W. A. (1988) Novel photocleavable protein cross-linking reagents and their use in the preparation of antibody-toxin conjugates. Photochem. Photobiol. 42, 231-237.
13. Duncan, R. J. S., and Weston, P. D. (1983) A new reagent which may be used to introduce sulfhydryl groups into proteins, and its use in the preparation of conjugates for immunoassay. Anal. Biochem. 132, 68-73.