A novel chromatographic method for Ep-CAM mRNA detection in peripheral blood and bone marrow of patients with metastatic colorectal cancer

Anticancer Research

Volumn 25, Issue 1 A, 2005, Pages 319-323

  Karanikiotis, Charisios ^a,d   Skiadas, Ioannis ^b   Karina, Maria ^c   Georgakopoulou, Stavroula ^b   Georgakopoulos, Eleftherios ^b   Fountzilas, George ^c  

^a 424 General Army Hospital   (Greece)

^b Acron Genomics Hellas SA   (Greece)

^c ARISTOTLE UNIVERSITY OF THESSALONIKI   (Greece)

^d NONE   (Greece)

Author keywords

Circulating tumor cells; Colorectal cancer; Ep CAM; Micrometastasis

Indexed keywords

EPITHELIAL CELL ADHESION MOLECULE; MESSENGER RNA;

AGAR GEL ELECTROPHORESIS; ARTICLE; BLOOD; BONE MARROW; CHROMATOGRAPHY; CLINICAL ARTICLE; COLORECTAL CANCER; CONTROLLED STUDY; HUMAN; HUMAN CELL; HUMAN TISSUE; METASTASIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROGNOSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

ANTIGENS, NEOPLASM; BONE MARROW; CELL ADHESION MOLECULES; CHROMATOGRAPHY; COLORECTAL NEOPLASMS; HUMANS; NEOPLASM METASTASIS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER;

EID: 16344367922     PISSN: 02507005     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (21)

1. Linnenbach AJ, Wojcierowski J, Wu SA et al: Sequence investigation of the major gastrointestinal tumor-associated antigen gene family, GA733. Proc Natl Acad Sci USA 86: 27-31, 1989.
2. Litvinov SV, Velders MP, Bakker HA et al: Ep-CAM: a human epithelial antigen is a homophilic cell-cell adhesion molecule. J Cell Biol 125: 437-446, 1994.
3. Basak S, Speicher D, Eck S et al: Colorectal carcinoma invasion inhibition by CO17-1A/GA733 antigen and its murine homologue. J Natl Cancer Inst 90: 691-697, 1998.
4. Litvinov SV, Balzar M, Winter MJ et al: Epithelial cell adhesion molecule (Ep-CAM) modulates cell-cell interaction mediated by classic cadherins. J Cell Biol 139: 1337-1348, 1997.
5. Gastl G, Spizzo G, Obrist P et al: Ep-CAM overexpression in breast cancer as a predictor of survival. Lancet 356: 1981-13822, 2000.
6. Spizzo G, Obrist P, Ensinger C et al: Prognostic significance of Ep-CAM AND Her-2/neu overexpression in invasive breast cancer. Int J Cancer 98: 883-888, 2002.
7. Spizzo G, Gastl G, Wolf D et al: Correlation of COX-2 and Ep-CAM overexpression in human invasive breast cancer and its impact on survival. Br J Cancer 88: 574-578, 2003.
8. Chaubal S, Wollenberg B, Kastenbauer E et al: Ep-CAM - a marker for the detection of disseminated tumor cells in patients suffering from SCCHN. Anticancer Res 19: 2237-2242, 1999.
9. Piyathilake CJ, Frost AR, Weiss H et al: The expression of Ep-CAM (17-1A) in squamous cell cancers of the lung. Hum Pathol 31: 482-487, 2000.
10. Poczatek RB, Myers RB, Manne U et al: Ep-Cam levels in prostatic adenocarcinoma and prostatic intraepithelial neoplasia. J Urol 762: 1462-1466, 1999.
11. Thurm H, Ebel S, Kentenich C et al: Rare expression of epithelial cell adhesion molecule on residual micrometastatic breast cancer cells after adjuvant chemotherapy. Clin Cancer Res 9: 2598-2604, 2003.
12. Bazar M, Winter MJ, de Boer CJ et al: The biology of the 17-1A antigen (Ep-CAM). J Mol Med 77: 699-712, 1999.
13. Helfrich W, ten Poele R, Meersma G et al: A quantitative reverse transcriptase polymerase chain reaction- based assay to detect carcinoma cells in peripheral blood. Br J Cancer 76: 29-35, 1997.
14. Schuster R, Max N, Mann B et al. Quantitative real-time RT-PCR for detection of disseminated tumor cells in peripheral blood of patients with colorectal cancer using different mRNA markers. Int J Cancer 108: 219-227, 2004.
15. Kienle P, Koch M, Autschbach F et al: Decreased detection rate of disseminated tumor cells of rectal cancer patients after preoperative chemoradiation: a first step towards a molecular surrogate marker for neoadjuvant treatment in colorectal cancer. Ann Surg 238: 324-330, 2003.
16. Guller U, Zajac P, Schnider A et al: Disseminated single tumor cells as detected by real-time quantitative polymerase chain reaction represent a prognostic factor in patients undergoing surgery for colorectal cancer. Ann Surg 236: 768-75, 2002.
17. Wharton R, Jonas S, Glover C et al: Increased detection of circulating tumor cells in the blood of colorectal carcinoma patients using two reverse transcription-PCR assays and multiple blood samples. Clin Cancer Res 5: 4158-4163, 1999.
18. Guadagni F, Kantor J, Aloe S et al: Detection of blood-borne cells in colorectal cancer patients by nested reverse transcription-polymerase chain reaction for carcinoembryonic antigen messenger RNA: Longitudinal analyses and demonstration of its potential importance as an adjunct to multiple serum markers. Cancer Res 61: 2523-2532, 2001.
19. Jonas S, Windeatt S, O-Boateng A et al: Identification of carcinoembryonic antigen-producing cells circulating in the blood of patients with colorectal carcinoma by reverse transcriptase polymerase chain reaction. Gut 39: 717-721, 1996.
20. Wyld D, Selby P, Perren T et al: Detection of colorectal cancer cells in peripheral blood by reverse-transcriptase polymerase chain reaction for cytoceratin 20. Int J Cancer 79: 288-293, 1998.
21. Riethmuller G, Holz E, Schlimok G et al: Monoclonal antibody therapy for resected Dukes' C colorectal cancer: seven-year outcome of a multicenter randomized trial. J Clin Oncol 16: 1788-1794, 1998.