Adaption of neutral protease activity for islet isolation from the long-term two-layer method-stored pig pancreas

Transplantation Proceedings

Volumn 37, Issue 1, 2005, Pages 458-459

  Brandhorst, D ^a   Iken, M ^a   Brendel, M D ^a   Bretzel, R G ^a   Brandhorst, H ^a  

^a UNIVERSITY HOSPITAL GIESSEN   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

TRYPAN BLUE;

ADAPTATION; ANIMAL CELL; CELL FUNCTION; CELL ISOLATION; CELL VIABILITY; CONFERENCE PAPER; CONTROLLED STUDY; DISSOLUTION; ENZYME ACTIVITY; GLUCOSE BLOOD LEVEL; NONHUMAN; PANCREAS; PANCREAS ISLET TRANSPLANTATION; PRIORITY JOURNAL; QUALITY CONTROL; REDUCTION; SWINE;

EID: 16244385925     PISSN: 00411345     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.transproceed.2004.12.035     Document Type: Conference Paper

References (6)

1. T. Tsujimura, Y. Kuroda, T. Kin Human islet transplantation from pancreases with prolonged cold ischemia using additional preservation by the two-layer (UW solution/perfluorochemical) cold-storage method Transplantation 74 2002 1687
2. J. Mellert, B.J. Hering, U.T. Hopt Exchange of pancreata and islets between centers for experimental islet transplantation in the pig Transplant Proc 23 1991 2435
3. J.H. van Deijnen, C.E. Hulstaert, G.H. Wolters Significance of the peri-insular extracellular matrix for islet isolation from the pancreas of rat, dog, pig, and man Cell Tissue Res 267 1992 139
4. G.H.J. Wolter, G.H. Vos-Schepenkeuter, J.H.M. van Deijnen An analysis of the role of collagenase and protease in the enzymatic dissociation of the rat pancreas for islet isolation Diabetologia 35 1992 735
5. 2) method of pancreas preservation on human islet isolation, as assessed by the Edmonton Isolation Protocol Transplantation 74 2002 1414
6. H. Brandhorst, D. Brandhorst, B.J. Hering Significant progress in porcine islet mass isolation utilizing Liberase HI for enzymatic low temperature pancreas digestion Transplantation 68 1999 355