Synthesis of cotelomers derived from tris(hydroxymethyl)acrylamidomethane (THAM) bearing cytosine arabinoside moieties. Preliminary investigation of their biological activity

Tetrahedron Letters

Volumn 37, Issue 50, 1996, Pages 9049-9052

  Contino, Christiane ^a   Ollier, Monique ^b   Maurizis, Jean Claude ^b   Lacombe, Jean Michel ^a   Pucci, Bernard ^a  

^a UNIVERSITY OF AVIGNON   (France)

^b INSERM   (France)

Author keywords

[No Author keywords available]

Indexed keywords

CYTARABINE DERIVATIVE; DRUG CARRIER; TROMETAMOL;

ANIMAL CELL; ARTICLE; CHEMICAL REACTION; CYTOTOXICITY; DRUG ACTIVITY; DRUG SYNTHESIS; HYDROLYSIS; MELANOMA CELL; MOUSE; NONHUMAN; TELOMERE;

ARA;

EID: 16144363111     PISSN: 00404039     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0040-4039(96)02122-3     Document Type: Article

References (15)

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14. 14/ For example, x is determined by comparing peak areas assigned to the H1' proton of the ARA-C ribose (δ = 6.05 ppm ; integral = n H) with the THAM hydroxyle protons (δ = 5, ppm, integral = 3 nx H).For the telomers bearing a hydrocarbon tail, n is evaluated by comparing the signal area due to the three protons of the terminal methyle (δ = 0.85 ppm ; Integral = 3H) to the area of the ribose proton H1'. In the case of fluorocarbon compounds, the elemental analysis allow us to calculate the ratio %N/%F. Keeping in mind the x previously evaluated, n is determined following the equation : (formula presented)
15. 2 incubator. After this time, all dishes were rinsed with phosphate buffer saline 0.05M pH 7,4, cells were fixed with methanol and stained with 0,2% crystal violet solution and colonies (>50 cells) were counted.