Quantification of ALS1 gene expression in Candida albicans biofilms by RT-PCR using hybridisation probes on the LightCycler™

Molecular and Cellular Probes

Volumn 19, Issue 3, 2005, Pages 153-162

  O'Connor, Louise ^a   Lahiff, Sinéad ^a   Casey, Fiona ^a   Glennon, Maura ^a   Cormican, Martin ^b   Maher, Majella ^a  

^a NATIONAL UNIVERSITY OF IRELAND   (Ireland)

^b UNIVERSITY HOSPITAL GALWAY   (Ireland)

Author keywords

ALS1; Biofilms; Candida albicans; Gene expression; Hybridisation probes; LightCycler

Indexed keywords

AGGLUTININ; SILASTIC;

ANALYTICAL EQUIPMENT; ARTICLE; BIOFILM; CANDIDA ALBICANS; CELL ADHESION; CONTROLLED STUDY; ENDOTHELIUM CELL; FUNGAL GENE; FUNGUS GROWTH; GENE EXPRESSION; GENE EXPRESSION REGULATION; GENE FUNCTION; HYBRIDIZATION; MOLECULAR PROBE; NONHUMAN; NUCLEOTIDE SEQUENCE; PLANKTON; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; UNINDEXED SEQUENCE;

CANDIDA ALBICANS;

EID: 15944374313     PISSN: 08908508     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mcp.2004.10.007     Document Type: Article

References (19)

1. H. Chang, S. Leaw, A. Huang, L. Wu, and T. Chang Rapid identification of yeasts in positive blood cultures by a multiplex PCR method J Clin Micro 39 10 2001 3466 3471
2. L.J. Douglas Candida biofilms and their role in infection Trends Microbiol 11 1 2003 30 36
3. S.P. Hawser, and L.J. Douglas Resistance of Candida albicans biofilms to antifungal agents in vitro Antimicrob Agents Chemother 39 1995 2128 2131
4. G. Ramage, S. Bachman, T.F. Patterson, B.L. Wickes, and J.L. Lopez-Ribot Investigation of multidrug efflux pumps in relation to fluconazole resistance in Candida albicans biofilms J Antimicrob Chemother 49 2002 973 998
5. R.A. Calderone Introduction and historical perspectives R.A. Calderone Candida and candidiasis 2002 ASM Press 3 13
6. E.M. Kojic, and R.O. Darouiche Candida infections of medical devices Clin Micro Rev 17 2 2004 255 267
7. J. Chandra, D. Kuhn, P.K. Mukherjee, L.L. Hoyer, T. McCormick, and M.A. Ghannoum Biofilm formation by the fungal pathogen Candida albicans: development, architecture, and drug resistance J Bacteriol 183 18 2001 5385 5394
8. S.P. Hawser, and L.J. Douglas Biofilm formation by Candida species on the surface of catheter materials in vitro Infect Immun 62 1994 915 921
9. G.S. Baillie, and L.J. Douglas Role of diorphism in the development of Candida albicans biofilms J Med Microbiol 44 1999 1019 1028
10. S. García-Sáchez, S. Aubert, I. Iraqui, G. Janbon, J.-M. Ghigo, and C. d'Enfert Candida albicans biofilms: a developmental state associated with specific and stable gene expression patterns Eukaryot Cell 3 2 2004 536 545
11. D.M. Kuhn, J. Chandra, P.K. Mukherjee, and M.A. Ghannoum Comparison of biofilms formed by Candida albicans and Candida parapsilosis on bioprosthetic surfaces Infect Immun 70 2002 878 888
12. L.L. Hoyer The ALS gene family of Candida albicans Trends Microbiol 9 4 2001 176 180
13. C.B. Green, G. Cheng, J. Chandra, P. Mukherjee, M. Ghannoum, and L.L. Hoyer RT-PCR detection of Candida albicans ALS gene expression in the reconstituted human epithelium [RHE] model of oral candidiasis and in model biofilms Microbiology 150 2004 267 275
14. S. Fronhoffs, G. Totzke, S. Stier, N. Wernert, M. Rothe, and T. Brüning A method for the rapid construction of cRNA standard curves in quantitative real-time reverse transcription polymerase chain reaction Mol Cell Probes 16 2002 99 110
15. J.P. Vanden Heuvel, F.L. Tyson, and D.A. Bell Construction of recombinant RNA templates for use as internal standards in quantitative RT-PCR Biotechniques 14 1993 395 398
16. R.A. Calderone, and P.C. Braun Adherance and receptor relationships of Candida albicans Microbiol Rev 55 1991 1 20
17. X. Li, Z. Yan, and J. Xu Quantitative variation of biofilms among strains in natural populations of Candida albicans Microbiology 149 2003 353 362
18. J.H. Shin, S.J. Kee, M.G. Shin, S.H. Kim, D.H. Shin, and S.K. Lee Biofilm production by isolates of Candida species recovered from nononuetropenic patients: comparison of bloodstream isolates with isolates from other sources J Clin Microbiol 40 2002 1244 1248
19. C. Alberti-Segui, A.J. Morales, H. Xing, M.M. Kessler, D.A. Willins, and K.G. Weinstock Identification of potential cell-surface proteins in Candida albicans and investigation of the role of a putative cell-surface glycosidase in adhesion and virulence Yeast 21 4 2004 285 302