A combined microsatellite multiplexing and boiling DNA extraction method for high-throughput parentage analyses in the Pacific oyster (Crassostrea gigas)

Aquaculture Research

Volumn 36, Issue 5, 2005, Pages 516-518

  Taris, Nicolas ^a   Baron, Sophie ^b   Sharbel, Timothy F ^a   Sauvage, Christopher ^a   Boudry, Pierre ^a  

^a Inst Francais Rech Pour l'E   (France)

^b Lab d'Analyses Genetiques GENINDEXE   (France)

Author keywords

Aquaculture; High throughput; Larval DNA extraction; Multiplex; Oysters; Parental assignment

Indexed keywords

OYSTER CULTURE;

CRASSOSTREA GIGAS; OSTREIDAE;

EID: 15944372316     PISSN: 1355557X     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1365-2109.2004.01208.x     Document Type: Article

References (16)

1. Boudry P., Collet B., Cornette F., Hervouet V. & Bonhomme F. (2002) High variance in reproductive success of the Pacific oyster (Crassostrea gigas, Thunberg) revealed by microsatellite-based parentage analysis of multifactorial crosses. Aquaculture 204, 283-296.
2. Estoup A., Largiader C.R., Perrot E. & Chourrout D. (1996) Rapid one-tube DNA extraction for reliable PCR detection of fish polymorphic markers and transgenes. Molecular Marine Biology and Biotechnology 5, 295-298.
3. Hara M. & Sekino M. (2003) Efficient detection of parentage in a cultured Japanese flounder Paralichthys olivaceus using microsatellite DNA marker. Aquaculture 217, 107-114.
4. Henegariu O., Heerema N.A., Dlouhy S.R., Vance G.H. & Vogt P.H. (1997) Multiplex PCR: critical parameters and step-by-step protocol. Research Reports. Biotechniques 23, 504-511.
5. Huvet A., Boudry P., Ohresser M., Delsert C. & Bonhomme F. (2000) Variable microsatellites in the Pacific oyster Crassostrea gigas and the other cupped oyster species. Animal Genetics 31, 71-72.
6. Huvet A., Fabioux C., McCombie H., Lapègue S. & Boudry P. (2004) Natural hybridization in genetically differentiated populations of Crassostrea gigas and C. angulata high-lighted by sequence variation in flanking regions of a microsatellite locus. Marine Ecology Progress Series 272, 141-152.
7. Jarne P. & Lagoda P.J.L. (1996) Microsatellites, from molecules to populations and back. Trends in Ecology and Evolution 11, 424-429.
8. Launey S. & Hedgecock D. (2001) High genetic load in the Pacific oyster Crassostrea gigas. Genetics 159, 255-265.
9. Li G., Hubert S., Bucklin K., Ribes V. & Hedgecock D. (2003) Characterization of 79 microsatellite DNA markers in the Pacific oyster Crassostrea gigas. Molecular Ecology Notes 3, 228-232.
10. Magoulas A., Gjetvaj B., Terzoglou V. & Zouros E. (1998) Three polymorphic microsatellites in the Japanese oyster Crassostrea gigas (Thunberg). Animal Genetics 29, 69-70.
11. Marshall T.C., Slate J., Kruuk L. & Pemberton J.M. (1998) Statistical confidence for likelihood-based paternity inference in natural populations. Molecular Ecology 7. 639-655.
12. Masi P., Spagnoletti Zeuli P.L. & Donini P. (2003) Development and analysis of multiplex microsatellite markers sets in common bean (Phaseolus vulgaris L.). Molecular Breeding 11, 303-313.
13. Mousseau T.A., Ritland K. & Heath D.D. (1998) A novel method for estimating heritability using molecular markers. Heredity 80, 218-224.
14. Neff B.D., Fu P. & Gross M.R. (2000) Microsatellite multiplexing in fish. Transactions of the American Fisheries Society 129, 584-593.
15. Vandeputte M., Dupont N., Chatain B. & Chevassus B. (2001) Setting up a strain-testing design for the seabass, Dicentrarchus labrax: a simulation study. Aquaculture 202, 329-342.
16. Ziegle J.S., Su Y. Corcoran K.P., Mayrand P.E., Hoff L.B., Mcbridge L.J. & Kronich M.N. (1992) Application of automated DNA sizing technology for genotyping microsatellite loci. Genomics 14, 1026-1031.