Typing six major hepatitis C virus genotypes by polymerase chain reaction using primers derived from nucleotide sequences of the NS5 region

International Hepatology Communications

Volumn 4, Issue 5, 1996, Pages 263-267

  Hashimoto, Michie ^a,c   Chayama, Kazuaki ^a,b   Tubota, Akihito ^a,b   Kobayashi, Masahiro ^a,b   Saitou, Satosi ^a,b   Arase, Yasuji ^a,b   Ikeda, Kenji ^a,b   Kobayashi, Mariko ^a   Nakano, Akemi ^a   Takagi, Kimiko ^a   Koike, Hiromi ^a   Okamoto, Katsura ^a   Handa, Hiroshi ^c   Kumada, Hiromitsu ^a,b  

^a TORANOMON HOSPITAL   (Japan)

^b Okinaka Memorial Institute for Medical Research   (Japan)

^c TOKYO INSTITUTE OF TECHNOLOGY   (Japan)

Author keywords

HCV; NS5 region; Nucleotide sequences

Indexed keywords

PRIMER DNA;

ADULT; ARTICLE; CONTROLLED STUDY; GENOTYPE; HEPATITIS C; HEPATITIS C VIRUS; HUMAN; HUMAN TISSUE; MAJOR CLINICAL STUDY; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL;

EID: 15844401380     PISSN: 09284346     EISSN: None     Source Type: Journal    
DOI: 10.1016/0928-4346(96)00257-5     Document Type: Article

References (7)

1. Tubota A, Chayama K, Ikeda K, et al. Factors predictive of response to interferon-therapy in hepatitis C virus infection. Hepatology 1994; 19: 1088-1094.
2. Chayama K, Tubota A, Arase Y, et al. Genotypic subtyping of hepatitis C virus. J Gastroenterol Hepatol 1993; 8: 150-156.
3. Okamoto H, Sugiyama Y, Okada S, et al. Typing hepatitis C virus by polymerase chain reaction with type-specific primers: application to clinical surveys and tracing infections sources. J Gen Virol 1992; 73: 673-679.
4. Okamoto H, Tokita H, Sakamoto M, et al. Characterization of the genomic sequence of type V (or 3a) hepatitis C virus isolates and PCR primers for specific detection. J Gen Virol 1993; 74: 2385-2390.
5. Hashimoto M, Chayama K, Tsubota A, et al. Genotyping of hepatitis C virus by the polymerase chain reaction: a comparison of two PCR-based methods using core or NS5 primers. Int Hepatol Commun 1995; 4: 67-71.
6. Simmonds P, Holmes EC, Cha T-A, et al. Classification of hepatitis C virus into six major genotypes and a series of subtypes by phylogenetic analysis of the NS-5 region. J Gen Virol 1993; 74: 2391-2399.
7. Mori S, Kato N, Yagyu A, et al. A new type of hepatitis C virus in patients in Thailand. Biochem Biophys Res Commun 1992; 183: 334-342.